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However, the relationship between their morphology and behavior under varying conditions remains poorly understood. This study provides novel insights by linking ZnO NPs shape to generation of reactive oxygen species (ROS), and to antimicrobial efficacy under varying temperatures. ROS generation was confirmed via electron paramagnetic resonance, although no antioxidant activity was observed. Antibacterial tests against Escherichia coli and Staphylococcus aureus at different temperatures (4\u201322 \u00b0C) revealed that sheet-shaped NPs achieved complete bacterial reduction (7.5 log CFU mL\u22121 for E. coli at 4 and 22 \u00b0C; 6.8 log CFU mL\u22121 for S. aureus at 22 \u00b0C). Flower-shaped NPs were less effective due to larger size and reduced surface area. Zeta potential ranged from \u221244 to \u221258 mV, indicating high stability, with sheet-shaped particles being the most dispersed. Scanning electron microscopy confirmed closer interaction between sheet-shaped NPs and E. coli in agreement with the higher activity. Antibacterial efficacy decreased at 4 \u00b0C, highlighting implications for cold storage. The Weibull model successfully described E. coli reduction. These aspects were not previously addressed in the published work. The effect of temperature on the activity and its modeling are new insights into the morphology-dependent antimicrobial activity of ZnO NPs, supporting their integration into packaging materials for food applications.<\/jats:p>","DOI":"10.3390\/nano15120892","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T05:11:49Z","timestamp":1749532309000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Functional Properties and Safety Considerations of Zinc Oxide Nanoparticles Under Varying Conditions"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8555-8518","authenticated-orcid":false,"given":"Ana Rita","family":"Mendes","sequence":"first","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0986-6607","authenticated-orcid":false,"given":"Carlos M.","family":"Granadeiro","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV & Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1967-8853","authenticated-orcid":false,"given":"Andreia","family":"Leite","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV & Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5371-3798","authenticated-orcid":false,"given":"Otmar","family":"Geiss","sequence":"additional","affiliation":[{"name":"European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7954-7532","authenticated-orcid":false,"given":"Ivana","family":"Bianchi","sequence":"additional","affiliation":[{"name":"European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4961-8405","authenticated-orcid":false,"given":"Jessica","family":"Ponti","sequence":"additional","affiliation":[{"name":"European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3482-2996","authenticated-orcid":false,"given":"Dora","family":"Mehn","sequence":"additional","affiliation":[{"name":"European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-5696","authenticated-orcid":false,"given":"Eul\u00e1lia","family":"Pereira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV & Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-5137","authenticated-orcid":false,"given":"Paula","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6598-6515","authenticated-orcid":false,"given":"F\u00e1tima","family":"Po\u00e7as","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica Portuguesa, CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"CINATE\u2014Laborat\u00f3rio de An\u00e1lises e Ensaios a Alimentos e Embalagens, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"ref_1","unstructured":"European Commission (2022). Commission Recommendation of 10 June 2022 on the Definition of Nanomaterial (Text with EEA Relevance) 2022\/C 229\/01. Off. J. Eur. Union, 229, 1\u20135."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ayeni, O., Oladokun, O., and Orodu, O.D. (2022). Nanotechnology: Applications, Challenges, and Prospects. Advanced Manufacturing in Biological, Petroleum, and Nanotechnology Processing, Springer.","DOI":"10.1007\/978-3-030-95820-6"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1002\/nano.202300038","article-title":"Nanomaterials: An Overview of Synthesis, Classification, Characterization, and Applications","volume":"4","author":"Mekuye","year":"2023","journal-title":"Nano Sel."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.tifs.2021.08.019","article-title":"Metal Oxide Nanoparticles for Safe Active and Intelligent Food Packaging","volume":"116","author":"Nikolic","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_5","unstructured":"Rodriguez, R. (2022). Active Packaging Systems Based on Metal and Metal Oxide Nanoparticles. Nanotechnology\u2014Enhanced Food Packaging, Wiley."},{"key":"ref_6","first-page":"100270","article-title":"Application of Nanotechnology in Food Packaging: Pros and Cons","volume":"7","author":"Ashfaq","year":"2022","journal-title":"J. Agric. Food Res."},{"key":"ref_7","unstructured":"L\u00f3pez-Rubio, M.\u00c1., Fabra, M.J., and Lagar\u00f3n, J.M. (2018). Overview on European Regulatory Issues, Legislation, and EFSA Evaluations of Nanomaterials. Nanomaterials for Food Packaging, Elsevier."},{"key":"ref_8","unstructured":"Nayak, S.K., and Mohanty, S. (2023). Polymer Composites as Packaging Materials. Industrial Applications of Polymer Composites, Bentham Science Publishers."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Mishra, B., Panda, J., Mishra, A.K., Nath, P.C., Nayak, P.K., Mahapatra, U., Sharma, M., Chopra, H., Mohanta, Y.K., and Sridhar, K. (2024). Recent Advances in Sustainable Biopolymer\u2014Based Nanocomposites for Smart Food Packaging: A Review. Int. J. Biol. Macromol., 279.","DOI":"10.1016\/j.ijbiomac.2024.135583"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.ijantimicag.2016.11.011","article-title":"Metal Oxide Nanoparticles as Antimicrobial Agents: A Promise for the Future","volume":"49","author":"Raghunath","year":"2017","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Anvar, A.A., Ahari, H., and Ataee, M. (2021). Antimicrobial Properties of Food Nanopackaging: A New Focus on Foodborne Pathogens. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.690706"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1080\/87559129.2020.1737709","article-title":"ZnO Nanostructures in Active Antibacterial Food Packaging: Preparation Methods, Antimicrobial Mechanisms, Safety Issues, Future Prospects, and Challenges","volume":"38","author":"Kim","year":"2022","journal-title":"Food Rev. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101201","DOI":"10.1016\/j.fpsl.2023.101201","article-title":"Antimicrobial Activity of In-Situ Bacterial Nanocellulose\u2014Zinc Oxide Composites for Food Packaging","volume":"40","author":"Carvalho","year":"2023","journal-title":"Food Packag. Shelf Life"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s40820-015-0040-x","article-title":"Review on Zinc Oxide Nanoparticles: Antibacterial Activity and Toxicity Mechanism","volume":"7","author":"Sirelkhatim","year":"2015","journal-title":"Nano-Micro Lett."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mendes, A.R., Granadeiro, C.M., Leite, A., Pereira, E., Teixeira, P., and Po\u00e7as, F. (2024). Optimizing Antimicrobial Efficacy: Investigating the Impact of Zinc Oxide Nanoparticle Shape and Size. Nanomaterials, 14.","DOI":"10.3390\/nano14070638"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1021\/jf051960d","article-title":"Modified ABTS Method to Measure Antioxidant Capacity of Selected Small Fruits and Comparison to FRAP and DPPH Methods","volume":"54","author":"Ozgen","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.foodres.2018.08.044","article-title":"Antimicrobial Activity of Pomegranate Peel Extracts Performed by High Pressure and Enzymatic Assisted Extraction","volume":"115","author":"Alexandre","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Huertas, J.P., Ros-Chumillas, M., Garre, A., Fern\u00e1ndez, P.S., Aznar, A., Iguaz, A., Esnoz, A., and Palop, A. (2021). Impact of Heating Rates on Alicyclobacillus acidoterrestris Heat Resistance Under Non-Isothermal Treatments and Use of Mathematical Modelling to Optimize Orange Juice Processing. Foods, 10.","DOI":"10.3390\/foods10071496"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100928","DOI":"10.1016\/j.cofs.2022.100928","article-title":"Application of Mathematical Models to Validate Emerging Processing Technologies in Food","volume":"48","author":"Alvarenga","year":"2022","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s12393-021-09291-y","article-title":"The Weibull Model for Microbial Inactivation","volume":"14","author":"Buzrul","year":"2022","journal-title":"Food Eng. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.ultramic.2017.07.001","article-title":"A Direct Comparison of Experimental Methods to Measure Dimensions of Synthetic Nanoparticles","volume":"182","author":"Eaton","year":"2017","journal-title":"Ultramicroscopy"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.jcis.2021.04.081","article-title":"Nanoparticle Size Distribution Quantification from Transmission Electron Microscopy (TEM) of Ruthenium Tetroxide Stained Polymeric Nanoparticles","volume":"604","author":"Wilson","year":"2021","journal-title":"J. Colloid Interface Sci."},{"key":"ref_23","unstructured":"Diez-Pascual, A.M. (2019). Chapter 11\u2014Characterization of Nanomaterials: Tools and Challenges. Nanomaterials for Food Applications, Elsevier."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.2147\/IJN.S19151","article-title":"Particle Size Reduction to the Nanometer Range: A Promising Approach to Improve Buccal Absorption of Poorly Water-Soluble Drugs","volume":"6","author":"Evans","year":"2011","journal-title":"Int. J. Nanomed."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Vinardell, M., Llanas, H., Marics, L., and Mitjans, M. (2017). In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide. Nanomaterials, 7.","DOI":"10.3390\/nano7030056"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.bbagen.2013.03.034","article-title":"Detection and Characterisation of Radicals in Biological Materials Using EPR Methodology","volume":"1840","author":"Hawkins","year":"2014","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0891-5849(01)00619-0","article-title":"Synthesis and Biochemical Applications of a Solid Cyclic Nitrone Spin Trap: A Relatively Superior Trap for Detecting Superoxide Anions and Glutathiyl Radicals","volume":"31","author":"Zhao","year":"2001","journal-title":"Free Radic. Biol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13551","DOI":"10.1007\/s10854-018-9483-4","article-title":"The Correlation Among Morphology, Oxygen Vacancies and Properties of ZnO Nanoflowers","volume":"29","author":"Hezam","year":"2018","journal-title":"J. Mater. Sci. Mater. Electron."},{"key":"ref_29","first-page":"90","article-title":"Bio-Synthesis, Characterization of ZnO Nanoparticles from Scoparia dulcis L. Plant Extract and Its In Vitro Antioxidant, Acetylcholinesterase Activity","volume":"10","author":"Mini","year":"2023","journal-title":"Plant Sci. Today"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1515\/gps-2022-0075","article-title":"Antioxidant and Photocatalytic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated Using the Aqueous Leaf Extract of Sida acuta","volume":"11","author":"Ramesh","year":"2022","journal-title":"Green Process. Synth."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.onano.2018.08.001","article-title":"Synthesis and Characterization of Zinc Oxide Nanoparticles Using Tuber Extract of Anchote (Coccinia abyssinica (Lam.) Cong.) for Antimicrobial and Antioxidant Activity Assessment","volume":"3","author":"Safawo","year":"2018","journal-title":"OpenNano"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102","DOI":"10.34172\/PS.2020.56","article-title":"Polyphenol Enriched Extract of Pomegranate Peel; A Novel Precursor for the Biosynthesis of Zinc Oxide Nanoparticles and Application in Sunscreens","volume":"27","author":"Kokabi","year":"2020","journal-title":"Pharm. Sci."},{"key":"ref_33","first-page":"117","article-title":"Review of the Environmentally Friendly Production of Zinc Oxide Nanoparticles and Its Antioxidant, Anti-Hyperlipidemic, and Anti-Diabetic Properties","volume":"10","author":"Ramana","year":"2023","journal-title":"J. Surv. Fish. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lee, J., Choi, K.-H., Min, J., Kim, H.-J., Jee, J.-P., and Park, B.J. (2017). Functionalized ZnO Nanoparticles with Gallic Acid for Antioxidant and Antibacterial Activity against Methicillin-Resistant S. aureus. Nanomaterials, 7.","DOI":"10.3390\/nano7110365"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3750","DOI":"10.1007\/s10904-023-02744-3","article-title":"Novel Green Synthesis of UV-Sunscreen ZnO Nanoparticles Using Solanum lycopersicum Fruit Extract and Evaluation of Their Antibacterial and Anticancer Activity","volume":"33","author":"Elbrolesy","year":"2023","journal-title":"J. Inorg. Organomet. Polym. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"20992","DOI":"10.1039\/D4RA02452D","article-title":"Cutting-Edge Developments in Zinc Oxide Nanoparticles: Synthesis and Applications for Enhanced Antimicrobial and UV Protection in Healthcare Solutions","volume":"14","author":"Irede","year":"2024","journal-title":"RSC Adv."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kumar, A., and Dixit, C.K. (2017). Methods for Characterization of Nanoparticles. Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids, Elsevier.","DOI":"10.1016\/B978-0-08-100557-6.00003-1"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/s11671-018-2532-3","article-title":"Properties of Zinc Oxide Nanoparticles and Their Activity Against Microbes","volume":"13","author":"Siddiqi","year":"2018","journal-title":"Nanoscale Res. Lett."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Babayevska, N., Przysiecka, \u0141., Iatsunskyi, I., Nowaczyk, G., Jarek, M., Janiszewska, E., and Jurga, S. (2022). ZnO Size and Shape Effect on Antibacterial Activity and Cytotoxicity Profile. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-12134-3"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.colsurfb.2012.07.033","article-title":"Influence of Size Scale and Morphology on Antibacterial Properties of ZnO Powders Hydrothermally Synthesized Using Different Surface Stabilizing Agents","volume":"102","year":"2013","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"10109","DOI":"10.1021\/acsami.5b11573","article-title":"Insight into Biological Effects of Zinc Oxide Nanoflowers on Bacteria: Why Morphology Matters","volume":"8","author":"Cai","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s12034-019-1986-y","article-title":"The Effect of Shape and Size of ZnO Nanoparticles on Their Antimicrobial and Photocatalytic Activities: A Green Approach","volume":"43","author":"Sharma","year":"2020","journal-title":"Bull. Mater. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ashwini, J., Aswathy, T.R., Rahul, A.B., Thara, G.M., and Nair, A.S. (2021). Synthesis and Characterization of Zinc Oxide Nanoparticles Using Acacia caesia Bark Extract and Its Photocatalytic and Antimicrobial Activities. Catalysts, 11.","DOI":"10.3390\/catal11121507"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Silhavy, T.J., Kahne, D., and Walker, S. (2010). The Bacterial Cell Envelope. Cold Spring Harb. Perspect. Biol., 2.","DOI":"10.1101\/cshperspect.a000414"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jphotobiol.2013.01.004","article-title":"Controllable Synthesis of ZnO Nanoparticles and Their Morphology-Dependent Antibacterial and Optical Properties","volume":"120","author":"Talebian","year":"2013","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Saliani, M., Jalal, R., and Kafshadre Goharshadi, E. (2015). Effects of pH and Temperature on Antibacterial Activity of Zinc Oxide Nanofluid Against E. coli O157:H7 and Staphylococcus aureus. Jundishapur J. Microbiol., 8.","DOI":"10.5812\/jjm.17115"},{"key":"ref_47","first-page":"296","article-title":"Effect of Dosage and Particle Size of Natural Zeolite on the Survival of Escherichia coli in Soil","volume":"4","author":"Sinegani","year":"2019","journal-title":"J. Water Environ. Nanotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1007\/s11051-009-9711-1","article-title":"Mechanistic Investigation into Antibacterial Behaviour of Suspensions of ZnO Nanoparticles Against E. coli","volume":"12","author":"Zhang","year":"2010","journal-title":"J. Nanopartic. Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Jiang, S., Lin, K., and Cai, M. (2020). ZnO Nanomaterials: Current Advancements in Antibacterial Mechanisms and Applications. Front. Chem., 8.","DOI":"10.3389\/fchem.2020.00580"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Ding, Z., Jiang, Y., and Liu, X. (2018). Nanoemulsions-Based Drug Delivery for Brain Tumors. Nanotechnology-Based Targeted Drug Delivery Systems for Brain Tumors, Elsevier.","DOI":"10.1016\/B978-0-12-812218-1.00012-9"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.apcbee.2014.01.003","article-title":"Particle Size and Zeta Potential of ZnO","volume":"9","author":"Marsalek","year":"2014","journal-title":"APCBEE Procedia"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"8221","DOI":"10.2147\/IJN.S339404","article-title":"Antiviral Activity of Zinc Oxide Nanoparticles Mediated by Plumbago indica L. Extract Against Herpes Simplex Virus Type 1 (HSV-1)","volume":"16","author":"Melk","year":"2021","journal-title":"Int. J. Nanomed."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Yassin, M.T., Mostafa, A.A.F., Al-Askar, A.A., and Al-Otibi, F.O. (2022). Facile Green Synthesis of Zinc Oxide Nanoparticles with Potential Synergistic Activity with Common Antifungal Agents Against Multidrug-Resistant Candidal Strains. Crystals, 12.","DOI":"10.3390\/cryst12060774"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101239","DOI":"10.1016\/j.mtchem.2022.101239","article-title":"Investigation of the Parameters Affecting the Morphology of Zinc Oxide (ZnO) Nanoparticles Synthesized by Precipitation Method","volume":"26","author":"Rezaei","year":"2022","journal-title":"Mater. Today Chem."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Arakha, M., Saleem, M., Mallick, B.C., and Jha, S. (2015). The Effects of Interfacial Potential on Antimicrobial Propensity of ZnO Nanoparticles. Sci. Rep., 5.","DOI":"10.1038\/srep09578"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1021\/acscentsci.9b01108","article-title":"The Relationship Between Static Charge and Shape","volume":"6","author":"Pandey","year":"2020","journal-title":"ACS Cent. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1016\/j.msec.2012.07.011","article-title":"From Zinc Oxide Nanoparticles to Microflowers: A Study of Growth Kinetics and Biocidal Activity","volume":"32","author":"Ramani","year":"2012","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"100125","DOI":"10.1016\/j.hazl.2024.100125","article-title":"Investigating the Toxicological Effects of Nanomaterials in Food Packaging Associated with Human Health and the Environment","volume":"5","author":"Gupta","year":"2024","journal-title":"J. Hazard. Mater. Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.2147\/IJN.S246484","article-title":"Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis","volume":"15","author":"Mazur","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Motelica, L., Vasile, B.-S., Ficai, A., Surdu, A.-V., Ficai, D., Oprea, O.-C., Andronescu, E., Jinga, D.C., and Holban, A.M. (2022). Influence of the Alcohols on the ZnO Synthesis and Its Properties: The Photocatalytic and Antimicrobial Activities. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14122842"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"77","DOI":"10.32598\/PBR.9.2.920.2","article-title":"The Exposure and Hazards of Zinc Oxide Nanoparticles: In Vitro and In Vivo Studies","volume":"9","author":"Malekshah","year":"2023","journal-title":"Pharm. Biomed. Res."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids) (2016). Scientific Opinion on the Safety Assessment of the Substance Zinc Oxide, Nanoparticles, for Use in Food Contact Materials. EFSA J., 14, 4408.","DOI":"10.2903\/j.efsa.2016.4408"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids) (2015). Scientific Opinion on the Safety Evaluation of the Substance Zinc Oxide, Nanoparticles, Uncoated and Coated with [3-(Methacryloxy)propyl] Trimethoxysilane, for Use in Food Contact Materials. EFSA J., 13, 4063.","DOI":"10.2903\/j.efsa.2015.4063"},{"key":"ref_64","unstructured":"European Commission (2016). Commission Regulation (EU) 2016\/1416 of 24 August 2016 Amending and Correcting Regulation (EU) No 10\/2011 on Plastic Materials and Articles Intended to Come into Contact with Food. Off. J. Eur. Union, 22\u201342."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Motelica, L., Ficai, D., Oprea, O., Ficai, A., Trusca, R.-D., Andronescu, E., and Holban, A.M. (2021). Biodegradable Alginate Films with ZnO Nanoparticles and Citronella Essential Oil\u2014A Novel Antimicrobial Structure. 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