{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T07:11:23Z","timestamp":1766733083595,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,3,9]],"date-time":"2019-03-09T00:00:00Z","timestamp":1552089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["OE\/SAU\/UI0038\/2014; UID\/BIM\/04293\/2013, UID\/IC\/00051\/2013, SFRH\/BPD\/90010\/2012"],"award-info":[{"award-number":["OE\/SAU\/UI0038\/2014; UID\/BIM\/04293\/2013, UID\/IC\/00051\/2013, SFRH\/BPD\/90010\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006135","name":"ERAB: The European Foundation for Alcohol Research","doi-asserted-by":"publisher","award":["EA 14 23"],"award-info":[{"award-number":["EA 14 23"]}],"id":[{"id":"10.13039\/501100006135","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Polyphenols present in some alcoholic beverages have been linked to beneficial effects in preventing cardiovascular diseases. Polyphenols found in beer with anti-proliferative and anti-cancer properties are appealing in the context of the quasi-malignant phenotype of pulmonary arterial hypertension (PAH). Our purpose was to evaluate if the chronic ingestion of a xanthohumol-fortified beer (FB) would be able to modulate the pathophysiology of experimental PAH. Male Wistar rats with monocrotaline (MCT)-induced PAH (60 mg\/kg) were allowed to drink either xanthohumol-fortified beer (MCT + FB) or 5.2% ethanol (MCT + SHAM) for a period 4 weeks. At the end of the protocol, cardiopulmonary exercise testing and hemodynamic recordings were performed, followed by sample collection for further analysis. FB intake resulted in a significant attenuation of the pulmonary vascular remodeling in MCT + FB animals. This improvement was paralleled with the downregulation in expression of proteins responsible for proliferation (ERK1\/2), cell viability (AKT), and apoptosis (BCL-XL). Moreover, MCT + FB animals presented improved right ventricle (RV) function and remodeling accompanied by VEGFR-2 pathway downregulation. The present study demonstrates that a regular consumption of xanthohumol through FB modulates major remodeling pathways activated in experimental PAH.<\/jats:p>","DOI":"10.3390\/nu11030583","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T03:49:31Z","timestamp":1552362571000},"page":"583","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Anti-Remodeling Effects of Xanthohumol-Fortified Beer in Pulmonary Arterial Hypertension Mediated by ERK and AKT Inhibition"],"prefix":"10.3390","volume":"11","author":[{"given":"Ana Filipa","family":"Silva","sequence":"first","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Gabriel","family":"Faria-Costa","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"F\u00e1bio","family":"Sousa-Nunes","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Manuel Filipe","family":"Santos","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2418-5890","authenticated-orcid":false,"given":"Manuel Jo\u00e3o","family":"Ferreira-Pinto","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Delfim","family":"Duarte","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina\u2014Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-7670","authenticated-orcid":false,"given":"Ilda","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina\u2014Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4836-6311","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Tiago Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina\u2014Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Patologia Cl\u00ednica, Centro Hospitalar Universit\u00e1rio S\u00e3o Jo\u00e3o, 4200-319 Porto, Portugal"},{"name":"Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, 4050-600 Porto, Portugal"}]},{"given":"Adelino","family":"Leite-Moreira","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Daniel","family":"Moreira-Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Centro de Actividade F\u00edsica Sa\u00fade e Lazer, Faculdade de Desporto, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Tiago","family":"Henriques-Coelho","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"Departamento de Ginecologia e Obstetr\u00edcia e Pediatria, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Rita","family":"Negr\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina\u2014Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"I3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.ijcard.2011.05.066","article-title":"Current pathophysiological concepts and management of pulmonary hypertension","volume":"155","author":"Lourenco","year":"2012","journal-title":"Int. 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