{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:49:07Z","timestamp":1771030147193,"version":"3.50.1"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T00:00:00Z","timestamp":1626134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Soremartec Italia S.r.l.","award":["xxx"],"award-info":[{"award-number":["xxx"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>The effect of coffee and cocoa on oxidative damage to macromolecules has been investigated in several studies, often with controversial results. This study aimed to investigate the effect of one-month consumption of different doses of coffee or cocoa-based products containing coffee on markers of DNA damage and lipid peroxidation in young healthy volunteers. Twenty-one volunteers were randomly assigned into a three-arm, crossover, randomized trial. Subjects were assigned to consume one of the three following treatments: one cup of espresso coffee\/day (1C), three cups of espresso coffee\/day (3C), and one cup of espresso coffee plus two cocoa-based products containing coffee (PC) twice per day for 1 month. At the end of each treatment, blood samples were collected for the analysis of endogenous and H2O2-induced DNA damage and DNA oxidation catabolites, while urines were used for the analysis of oxylipins. On the whole, four DNA catabolites (cyclic guanosine monophosphate (cGMP), 8-OH-2\u2032-deoxy-guanosine, 8-OH-guanine, and 8-NO2-cGMP) were detected in plasma samples following the one-month intervention. No significant modulation of DNA and lipid damage markers was documented among groups, apart from an effect of time for DNA strand breaks and some markers of lipid peroxidation. In conclusion, the consumption of coffee and cocoa-based confectionery containing coffee was apparently not able to affect oxidative stress markers. More studies are encouraged to better explain the findings obtained and to understand the impact of different dosages of these products on specific target groups.<\/jats:p>","DOI":"10.3390\/nu13072399","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T22:25:31Z","timestamp":1626215131000},"page":"2399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8298-926X","authenticated-orcid":false,"given":"Daniela","family":"Martini","sequence":"first","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-712X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Dom\u00ednguez-Perles","sequence":"additional","affiliation":[{"name":"Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8533-797X","authenticated-orcid":false,"given":"Alice","family":"Rosi","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]},{"given":"Michele","family":"Tassotti","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5436-7428","authenticated-orcid":false,"given":"Donato","family":"Angelino","sequence":"additional","affiliation":[{"name":"Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, 64100 Teramo, Italy"}]},{"given":"Sonia","family":"Medina","sequence":"additional","affiliation":[{"name":"Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4113-8682","authenticated-orcid":false,"given":"Cristian","family":"Ricci","sequence":"additional","affiliation":[{"name":"Pediatric Epidemiology, Department of Pediatrics, University Medicine Leipzig, 04103 Leipzig, Germany"}]},{"given":"Alexandre","family":"Guy","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, University of Montpellier, CNRS, ENSCM, 34093 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5177-5792","authenticated-orcid":false,"given":"Camille","family":"Oger","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, University of Montpellier, CNRS, ENSCM, 34093 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7739-2378","authenticated-orcid":false,"given":"Letizia","family":"Gigliotti","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"given":"Thierry","family":"Durand","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, University of Montpellier, CNRS, ENSCM, 34093 Montpellier, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9913-4380","authenticated-orcid":false,"given":"Mirko","family":"Marino","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"given":"Hans","family":"Gottfried-Genieser","sequence":"additional","affiliation":[{"name":"BIOLOG Life Science Institute, 28199 Bremen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2693-3311","authenticated-orcid":false,"given":"Marisa","family":"Porrini","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"given":"Monica","family":"Antonini","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8666-4849","authenticated-orcid":false,"given":"Alessandra","family":"Dei Cas","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy"}]},{"given":"Riccardo C.","family":"Bonadonna","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy"}]},{"given":"Federico","family":"Ferreres","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition, Molecular Recognition and Encapsulation (REM) Group, Universidad Cat\u00f3lica de Murcia, UCAM, 30107 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5372-8658","authenticated-orcid":false,"given":"Francesca","family":"Scazzina","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8441-4611","authenticated-orcid":false,"given":"Furio","family":"Brighenti","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9204-7257","authenticated-orcid":false,"given":"Patrizia","family":"Riso","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7562-377X","authenticated-orcid":false,"given":"Cristian","family":"Del Bo\u2019","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences (DeFENS), Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2150-2977","authenticated-orcid":false,"given":"Pedro","family":"Mena","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7646-0386","authenticated-orcid":false,"given":"Angel","family":"Gil-Izquierdo","sequence":"additional","affiliation":[{"name":"Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5394-1259","authenticated-orcid":false,"given":"Daniele","family":"Del Rio","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"De Melo Pereira, G.V., de Carvalho Neto, D.P., J\u00fanior, A.I.M., do Prado, F.G., Pagnoncelli, M.G.B., Karp, S.G., and Soccol, C.R. 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