{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T15:28:17Z","timestamp":1776180497421,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T00:00:00Z","timestamp":1637280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n Seneca","doi-asserted-by":"publisher","award":["N# 20855\/PI\/18"],"award-info":[{"award-number":["N# 20855\/PI\/18"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nutrients"],"abstract":"<jats:p>Cruciferous sprouts are rising in popularity as a hallmark of healthy diets, partially because of their phytochemical composition, characterized by the presence of flavonols and cinnamates. However, to shed light on their biological activity, the ability to assimilate (poly)phenols from sprouts (bioaccessible fraction) during gastrointestinal digestion needs to be studied. In this frame, the present work studies the effect of the physicochemical and enzymatic characteristics of gastrointestinal digestion on flavonols and cinnamoyl derivatives, by a simulated static in vitro model, on different cruciferous (red radish, red cabbage, broccoli, and white mustard) sprouts. The results indicate that, although the initial concentrations of phenolic acids in red radish (64.25 mg\/g fresh weight (fw)) are lower than in the other sprouts studied, their bioaccessibility after digestion is higher (90.40 mg\/g fw), followed by red cabbage (72.52 mg\/g fw), white mustard (58.72 mg\/g fw), and broccoli (35.59 mg\/g fw). These results indicate that the bioaccessibility of (poly)phenols is not exclusively associated with the initial concentration in the raw material, but that the physico-chemical properties of the food matrix, the presence of other additional molecules, and the specific characteristics of digestion are relevant factors in their assimilation.<\/jats:p>","DOI":"10.3390\/nu13114140","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T08:29:17Z","timestamp":1637310557000},"page":"4140","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts"],"prefix":"10.3390","volume":"13","author":[{"given":"\u00c1ngel","family":"Abell\u00e1n","sequence":"first","affiliation":[{"name":"Phytochemistry and Healthy Foods Lab (LabFAS), Food Science and Technology Department (CEBAS-CSIC), University Campus of Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-712X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Dom\u00ednguez-Perles","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Foods Lab (LabFAS), Food Science and Technology Department (CEBAS-CSIC), University Campus of Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-3917","authenticated-orcid":false,"given":"Cristina","family":"Garc\u00eda-Viguera","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Foods Lab (LabFAS), Food Science and Technology Department (CEBAS-CSIC), University Campus of Espinardo, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-8764","authenticated-orcid":false,"given":"Diego A.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Foods Lab (LabFAS), Food Science and Technology Department (CEBAS-CSIC), University Campus of Espinardo, 30100 Murcia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Neugart, S., Majer, P., Schreiner, M., and Hideg, \u00c9. (2021). Blue Light Treatment but Not Green Light Treatment After Pre-exposure to UV-B Stabilizes Flavonoid Glycoside Changes and Corresponding Biological Effects in Three Different Brassicaceae Sprouts. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.611247"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rad\u00fcnz, M., Hackbart, H.C.D.S., Bona, N.P., Pedra, N.S., Hoffmann, J.F., Stefanello, F.M., and Da Rosa Zavareze, E. (2020). Glucosinolates and phenolic compounds rich broccoli extract: Encapsulation by electrospraying and antitumor activity against glial tumor cells. Colloids Surf. B Biointerfaces, 192.","DOI":"10.1016\/j.colsurfb.2020.111020"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Abell\u00e1n, \u00c1., Dom\u00ednguez-Perles, R., Moreno, D.A., and Garc\u00eda-Viguera, C. (2019). Sorting out the Value of Cruciferous Sprouts as Sources of Bioactive Compounds for Nutrition and Health. Nutrients, 11.","DOI":"10.3390\/nu11020429"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ramirez, D., Abell\u00e1n-Victorio, A., Beretta, V., Camargo, A., and Moreno, D.A. (2020). Functional Ingredients from Brassicaceae Species: Overview and Perspectives. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21061998"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"350","DOI":"10.3389\/fchem.2021.664602","article-title":"Sinapic Acid and Sinapate Esters in Brassica: Innate Accumulation, Biosynthesis, Accessibility via Chemical Synthesis or Recovery from Biomass, and Biological Activities","volume":"9","author":"Nguyen","year":"2021","journal-title":"Front. Chem."},{"key":"ref_6","first-page":"45","article-title":"Effect of hydrothermal processing on phenolic acids and flavonols contents in selected brassica vegetables","volume":"11","author":"Sikora","year":"2012","journal-title":"Acta Sci. Pol. Technol. Aliment."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Dom\u00ednguez-Perles, R., Baenas, N., and Garc\u00eda-Viguera, C. (2020). New Insights in (Poly)phenolic Compounds: From Dietary Sources to Health Evidence. Foods, 9.","DOI":"10.3390\/foods9050543"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1039\/C7FO00574A","article-title":"Bioaccessibility and bioavailability of phenolic compounds in bread: A review","volume":"8","author":"Angelino","year":"2017","journal-title":"Food Funct."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Corona-Leo, L.S., Meza-M\u00e1rquez, O.G., and Hern\u00e1ndez-Mart\u00ednez, D.M. (2021). Effect of in vitro digestion on phenolic compounds and antioxidant capacity of different apple (Malus domestica) varieties harvested in Mexico. Food Biosci., 43.","DOI":"10.1016\/j.fbio.2021.101311"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Majdoub, Y.O.E., Ginestra, G., Mandalari, G., Dugo, P., Mondello, L., and Cacciola, F. (2021). The Digestibility of Hibiscus sabdariffa L. Polyphenols Using an In Vitro Human Digestion Model and Evaluation of Their Antimicrobial Activity. Nutrients, 13.","DOI":"10.3390\/nu13072360"},{"key":"ref_11","first-page":"433","article-title":"Effect of in vitro gastrointestinal digestion on phenolic compounds and the antioxidant activity of Aloe vera","volume":"7","author":"Laib","year":"2020","journal-title":"Acta Sci. Nat."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"S\u0119czyk, \u0141., Sugier, D., \u015awieca, M., and Gawlik-Dziki, U. (2021). The effect of in vitro digestion, food matrix, and hydrothermal treatment on the potential bioaccessibility of selected phenolic compounds. Food Chem., 344.","DOI":"10.1016\/j.foodchem.2020.128581"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.tifs.2017.07.006","article-title":"Bioaccessibility of bioactive compounds from fruits and vegetables after thermal and nonthermal processing","volume":"67","author":"Barba","year":"2017","journal-title":"Trends Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/1541-4337.12041","article-title":"Sinapic Acid and Its Derivatives: Natural Sources and Bioactivity","volume":"13","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Abell\u00e1n, A., Dom\u00ednguez-Perles, R., Garc\u00eda-Viguera, C., and Moreno, D.A. (2021). Evidence on the Bioaccessibility of Glucosinolates and BreakDown Products of Cruciferous Sprouts by Simulated In Vitro Gastrointestinal Digestion. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222011046"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food\u2014An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s41596-018-0119-1","article-title":"INFOGEST static in vitro simulation of gastrointestinal food digestion","volume":"14","author":"Brodkorb","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"D\u0119bski, H., Wiczkowski, W., Szabli\u0144ska-Piernik, J., and Horbowicz, M. (2021). The Application of Fe-EDTA and Sodium Silicate Affects the Polyphenols Content in Broccoli and Radish Sprouts. Biomolecules, 11.","DOI":"10.3390\/biom11081190"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1002\/jsfa.8037","article-title":"Effects of seed priming, salinity and methyl jasmonate treatment on bioactive composition of Brassica oleracea var. capitata (white and red varieties) sprouts","volume":"97","author":"Hassini","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11409","DOI":"10.1021\/jf302863c","article-title":"Selecting sprouts of brassicaceae for optimum phytochemical composition","volume":"60","author":"Baenas","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, Z., Lee, H., Liang, X., Dong, L., Wang, Q., Huang, D., and Ong, C. (2018). Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables. Molecules, 23.","DOI":"10.3390\/molecules23051139"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Imran, M., Salehi, B., Sharifi-Rad, J., Aslam Gondal, T., Saeed, F., Imran, A., Shahbaz, M., Tsouh Fokou, P.V., Umair Arshad, M., and Khan, H. (2019). Kaempferol: A Key Emphasis to Its Anticancer Potential. Molecules, 24.","DOI":"10.3390\/molecules24122277"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/ejlt.200500292","article-title":"Antioxidative effect of main sinapic acid derivatives from rapeseed and mustard oil by-products","volume":"108","author":"Thiyam","year":"2006","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hussain, S., Rehman, A.U., Luckett, D.J., Blanchard, C.L., Obied, H.K., and Strappe, P. (2019). Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010001"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Tian, W., Hu, R., Chen, G., Zhang, Y., Wang, W., and Li, Y. (2021). Potential bioaccessibility of phenolic acids in whole wheat products during in vitro gastrointestinal digestion and probiotic fermentation. Food Chem., 362.","DOI":"10.1016\/j.foodchem.2021.130135"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zhu, Y., and Sang, S. (2017). Phytochemicals in whole grain wheat and their health-promoting effects. Mol. Nutr. Food Res., 61.","DOI":"10.1002\/mnfr.201600852"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"45","DOI":"10.31883\/pjfns\/116590","article-title":"Red Beetroot Juice Phytochemicals Bioaccessibility: An In Vitro Approach","volume":"70","author":"Deseva","year":"2020","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/1541-4337.12081","article-title":"In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility","volume":"13","author":"Alminger","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.foodchem.2010.05.105","article-title":"Matrix composition effect on the digestibility of carob flour phenols by an in-vitro digestion model","volume":"124","author":"Ortega","year":"2011","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.tifs.2016.10.022","article-title":"Interactions between polyphenols and polysaccharides: Mechanisms and consequences in food processing and digestion","volume":"60","author":"Renard","year":"2017","journal-title":"Trends Food Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1039\/C3FO60361J","article-title":"Phenolic compounds: Their journey after intake","volume":"5","author":"Chen","year":"2014","journal-title":"Food Funct."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/j.phytochem.2007.02.004","article-title":"Anthocyanins from red cabbage\u2014Stability to simulated gastrointestinal digestion","volume":"68","author":"McDougall","year":"2007","journal-title":"Phytochemistry"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.nupar.2020.12.003","article-title":"Effet de la digestion gastro-intestinale in vitro sur les compos\u00e9s ph\u00e9noliques et l\u2019activit\u00e9 antioxydante du th\u00e9 vert Camellia sinensis L. issu de l\u2019agriculture biologique","volume":"35","author":"Laib","year":"2021","journal-title":"Nutr. Clin. Et M\u00e9tabolisme"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/ajcn\/79.5.727","article-title":"Polyphenols: Food sources and bioavailability","volume":"79","author":"Manach","year":"2004","journal-title":"Am. J. Clin. Nutr."}],"container-title":["Nutrients"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/11\/4140\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:32:37Z","timestamp":1760167957000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6643\/13\/11\/4140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,19]]},"references-count":34,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["nu13114140"],"URL":"https:\/\/doi.org\/10.3390\/nu13114140","relation":{},"ISSN":["2072-6643"],"issn-type":[{"value":"2072-6643","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,19]]}}}