{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:11Z","timestamp":1760144231231,"version":"build-2065373602"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT project of IITAA","doi-asserted-by":"publisher","award":["UIDP\/00153\/2020"],"award-info":[{"award-number":["UIDP\/00153\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT -Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UI\/BD\/150890\/2021"],"award-info":[{"award-number":["UI\/BD\/150890\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ARM Program supported by DOE"},{"name":"ENA project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oceans"],"abstract":"<jats:p>Ocean Alkalinity Enhancement (OAE) is a promising carbon dioxide removal strategy, but its ecological impacts on marine microbial communities under varying nutrient conditions remain poorly understood. We conducted laboratory incubations using a natural North Atlantic microbial assemblage to investigate the response to OAE under both natural and nutrient-enriched regimes. We tracked phytoplankton and bacterioplankton dynamics, biomass, and leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) activity as indicators of organic matter remineralization. OAE consistently reduced phytoplankton abundance in both nutrient regimes, potentially due to CO2 limitation, resulting in lower production of phytoplankton-derived organic matter. This reduction was reflected in decreased LAP activity and shifts in the relative abundance of phytoplankton-associated bacterial taxa. These findings indicate that OAE can directly affect phytoplankton through carbonate chemistry alterations, with potential microbial responses largely mediated by changes in organic matter availability. While short-term microbial disruptions were modest, the ecological consequences of altered bloom dynamics should be carefully considered in future OAE deployment strategies.<\/jats:p>","DOI":"10.3390\/oceans6040065","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:47:08Z","timestamp":1760104028000},"page":"65","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Influence of Inorganic Nutrients on a North Atlantic Microbial Community\u2019s Response to Ocean Alkalinity Enhancement"],"prefix":"10.3390","volume":"6","author":[{"given":"In\u00eas","family":"de Castro","sequence":"first","affiliation":[{"name":"Group of Climate, Meteorology and Global Change, IITAA\u2014Institute of Agricultural and Environmental Research and Technology, University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5182-0082","authenticated-orcid":false,"given":"Susana C.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Group of Food Science and Health, IITA\u2014Institute of Agricultural and Environmental Research and Technology, University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"}]},{"given":"Ant\u00f3nio","family":"Louvado","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1934-0091","authenticated-orcid":false,"given":"Newton Carlos Marcial","family":"Gomes","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"given":"M\u00e1rio","family":"Cach\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto D. Luiz (IDL), Faculty of Sciences, University of Lisbon, Campo Grande Edif\u00edcio C1, Piso 1, 1749-016 Lisbon, Portugal"},{"name":"Department of Geology, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8042-4510","authenticated-orcid":false,"given":"Paulo F.","family":"Silva Borges","sequence":"additional","affiliation":[{"name":"Group of Climate, Meteorology and Global Change, IITAA\u2014Institute of Agricultural and Environmental Research and Technology, University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5172-7742","authenticated-orcid":false,"given":"Eduardo","family":"Brito de Azevedo","sequence":"additional","affiliation":[{"name":"Group of Climate, Meteorology and Global Change, IITAA\u2014Institute of Agricultural and Environmental Research and Technology, University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0879-2298","authenticated-orcid":false,"given":"Joana","family":"Barcelos e Ramos","sequence":"additional","affiliation":[{"name":"Group of Climate, Meteorology and Global Change, IITAA\u2014Institute of Agricultural and Environmental Research and Technology, University of the Azores, 9700-042 Angra do Hero\u00edsmo, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"ref_1","unstructured":"(2015). 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