{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T17:52:27Z","timestamp":1773510747778,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T00:00:00Z","timestamp":1609891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>(1) Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological anti-T. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening of the 2015 and 2016 sera, an increase of seroprevalence was observed (57.7% (95% CI: 49.9\u201365.3%) versus 69.1% (95% CI: 61.5\u201375.9), from 2015 and 2016, respectively) (p = 0.031). (4) Conclusions The present study is the first to provide prospective data on the anti-T. gondii serological status of a sheep cohort in Portugal, showing an increase in the occurrence of T. gondii. There is a need to provide a clearer understanding of T. gondii epidemiology in Portugal, ideally by implementing monitoring programs on sentinel herds, not only due to the high impact of T. gondii on animal health but also for it being a zoonosis.<\/jats:p>","DOI":"10.3390\/pathogens10010040","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T09:15:16Z","timestamp":1609924516000},"page":"40","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A 2-Year Longitudinal Seroepidemiological Evaluation of Toxoplasma gondii Antibodies in a Cohort of Autochthonous Sheep from Central Portugal"],"prefix":"10.3390","volume":"10","author":[{"given":"Daniela","family":"Almeida","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Quirino","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6954-1919","authenticated-orcid":false,"given":"Patr\u00edcia Ferreira","family":"Barradas","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal"},{"name":"Epidemiology Research Unit (EPIUnit), Instituto de Sa\u00fade P\u00fablica, University of Porto, 4050-091 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8384-0640","authenticated-orcid":false,"given":"Priscilla Gomes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Maria","family":"Pereira","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, 1349-008 Lisboa, Portugal"}]},{"given":"Rita","family":"Cruz","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for Studies in Education and Health Technologies (CI&amp;DETS), 3500-606 Viseu, Portugal"}]},{"given":"Carla","family":"Santos","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for Studies in Education and Health Technologies (CI&amp;DETS), 3500-606 Viseu, Portugal"}]},{"given":"Ana Cristina","family":"Mega","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal"}]},{"given":"Fernando","family":"Esteves","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for Studies in Education and Health Technologies (CI&amp;DETS), 3500-606 Viseu, Portugal"}]},{"given":"Carmen","family":"N\u00f3brega","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6829-4867","authenticated-orcid":false,"given":"Helena","family":"Vala","sequence":"additional","affiliation":[{"name":"Agrarian School of Viseu, Polytechnic Institute of Viseu (ESAV), 3500-606 Viseu, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5302-0086","authenticated-orcid":false,"given":"F\u00e1tima","family":"G\u00e4rtner","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal"},{"name":"Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), 4099-002 Porto, Portugal"},{"name":"Institute for Research and Innovation in Health (i3S), University of Porto, 4099-002 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2305-1631","authenticated-orcid":false,"given":"Irina","family":"Amorim","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, 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