{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:54:24Z","timestamp":1760230464803,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"High-level Talents in Zhongkai University of Agriculture and Engineering","award":["J2201080102","KTP20210313"],"award-info":[{"award-number":["J2201080102","KTP20210313"]}]},{"name":"Guangdong rural science and Technology Commissioner project","award":["J2201080102","KTP20210313"],"award-info":[{"award-number":["J2201080102","KTP20210313"]}]},{"name":"Higher education teaching reform project from Zhongkai University of Agriculture and Engineering","award":["J2201080102","KTP20210313"],"award-info":[{"award-number":["J2201080102","KTP20210313"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>High temperatures and the seasonality in tropical ecosystems favours plant pathogens, which result in many fungal diseases. Among these, diseases caused by Botryosphaeriaceae species are prominent as dieback, canker and leaf spots. In this research, we isolated one leaf-spot-causing Botryosphaeriaceae species from Ficus altissima leaves, which were collected in Guangzhou, Guangdong Province, China. Isolation and identification of the pathogen were based on morphological and molecular aspects. Based on multigene phylogenetic analysis of combined internal transcribed spacer (ITS), translation elongation factor 1-\u03b1 gene (tef1) and beta-tubulin gene (tub2), the fungus associated with leaf spots on F. altissima is described as Lasiodiplodia fici, a novel species. Pathogenicity assays were conducted by inoculating the fungus onto detached shoots and plants under controlled environmental conditions. The results revealed that the L. fici isolates can infect the plant tissues under stress conditions by developing disease symptoms on detached shoots within three days. However, when it was inoculated onto the leaves of the host and grown in natural conditions, the progression of the disease was slow. The putative pathogen was re-isolated, and Koch\u2019s assumptions were satisfied. This is the first report of Lasiodiplodia species causing disease on Ficus altissima. Results from the present study will provide additional knowledge on fungal pathogens associated with forest and ornamental plant species.<\/jats:p>","DOI":"10.3390\/pathogens11080840","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T03:21:16Z","timestamp":1658978476000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Lasiodiplodia fici sp. nov., Causing Leaf Spot on Ficus altissima in China"],"prefix":"10.3390","volume":"11","author":[{"given":"GuiYan","family":"Xia","sequence":"first","affiliation":[{"name":"Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand"},{"name":"School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5730-3596","authenticated-orcid":false,"given":"Ishara","family":"Manawasinghe","sequence":"additional","affiliation":[{"name":"Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-9784","authenticated-orcid":false,"given":"Alan","family":"Phillips","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Biosystems and Integrative Sciences Institute (BioISI), Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal"}]},{"given":"ChunPing","family":"You","sequence":"additional","affiliation":[{"name":"Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7702-4885","authenticated-orcid":false,"given":"Ruvishika","family":"Jayawardena","sequence":"additional","affiliation":[{"name":"Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand"},{"name":"School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand"}]},{"given":"Mei","family":"Luo","sequence":"additional","affiliation":[{"name":"Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"Key Laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, China"}]},{"given":"Kevin","family":"Hyde","sequence":"additional","affiliation":[{"name":"Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand"},{"name":"School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"ref_1","first-page":"563","article-title":"Biodiversity and ecosystem functioning in soil","volume":"26","author":"Brussaard","year":"1997","journal-title":"Ambio"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s13225-020-00458-2","article-title":"The numbers of fungi: Is the descriptive curve flattening?","volume":"103","author":"Hyde","year":"2020","journal-title":"Fungal Divers."},{"key":"ref_3","first-page":"147","article-title":"Trail of decryption of molecular research on Botryosphaeriaceae in woody plants","volume":"55","author":"Chethana","year":"2016","journal-title":"Phytopathol. 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