{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T17:16:26Z","timestamp":1778174186428,"version":"3.51.4"},"reference-count":67,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF)","award":["POCI-01-0247-FEDER-027578"],"award-info":[{"award-number":["POCI-01-0247-FEDER-027578"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Urinary tract infections (UTIs) are a common public health problem, mainly caused by uropathogenic Escherichia coli (UPEC). Patients with chronic UTIs are usually treated with long-acting prophylactic antibiotics, which promotes the development of antibiotic-resistant UPEC strains and may complicate their long-term management. D-mannose and extracts rich in D-mannose such as mannan oligosaccharides (MOS; D-mannose oligomers) are promising alternatives to antibiotic prophylaxis due to their ability to inhibit bacterial adhesion to urothelial cells and, therefore, infection. This highlights the therapeutic potential and commercial value of using them as health supplements. Studies on the effect of MOS in UTIs are, however, scarce. Aiming to evaluate the potential benefits of using MOS extracts in UTIs prophylaxis, their ability to inhibit the adhesion of UPEC to urothelial cells and its mechanism of action were assessed. Additionally, the expression levels of the pro-inflammatory marker interleukin 6 (IL-6) were also evaluated. After characterizing their cytotoxic profiles, the preliminary results indicated that MOS extracts have potential to be used for the handling of UTIs and demonstrated that the mechanism through which they inhibit bacterial adhesion is through the competitive inhibition of FimH adhesins through the action of mannose, validated by a bacterial growth impact assessment.<\/jats:p>","DOI":"10.3390\/pathogens12070885","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T01:53:57Z","timestamp":1688003637000},"page":"885","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Mannan Oligosaccharides Extracts in Uropathogenic Escherichia coli Adhesion in Human Bladder Cells"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-7092","authenticated-orcid":false,"given":"Margarida","family":"Faustino","sequence":"first","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8268-2137","authenticated-orcid":false,"given":"Sara","family":"Silva","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3121-4514","authenticated-orcid":false,"given":"Eduardo M.","family":"Costa","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"given":"Ana Margarida","family":"Pereira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0300-5635","authenticated-orcid":false,"given":"Joana Odila","family":"Pereira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"given":"Ana Sofia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0968-5574","authenticated-orcid":false,"given":"Carlos M. H.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"given":"Carla F.","family":"Pereira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9104-4604","authenticated-orcid":false,"given":"Joana","family":"Dur\u00e3o","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Amyris Bio Products Portugal, Unipessoal Lda, Rua Diogo Botelho, 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-3184","authenticated-orcid":false,"given":"Manuela E.","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5967-4200","authenticated-orcid":false,"given":"Ana P.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centro de Biotecnologia e Qu\u00edmica Fina\u2013Laborat\u00f3rio Associado, Universidade Cat\u00f3lica Portuguesa, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nrmicro3432","article-title":"Urinary Tract Infections: Epidemiology, Mechanisms of Infection and Treatment Options","volume":"13","author":"Walker","year":"2015","journal-title":"Nat. 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