{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:33:32Z","timestamp":1762432412457,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Center for Genetic Engineering and Biotechnology, Havana, Cuba","award":["20-05736S","110RT0541"],"award-info":[{"award-number":["20-05736S","110RT0541"]}]},{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal","award":["20-05736S","110RT0541"],"award-info":[{"award-number":["20-05736S","110RT0541"]}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["20-05736S","110RT0541"],"award-info":[{"award-number":["20-05736S","110RT0541"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008441","name":"CYTED Network INCOGARR","doi-asserted-by":"publisher","award":["20-05736S","110RT0541"],"award-info":[{"award-number":["20-05736S","110RT0541"]}],"id":[{"id":"10.13039\/501100008441","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The control of ticks through vaccination offers a sustainable alternative to the use of chemicals that cause contamination and the selection of resistant tick strains. However, only a limited number of anti-tick vaccines have reached commercial realization. In this sense, an antigen effective against different tick species is a desirable target for developing such vaccines. A peptide derived from the tick P0 protein (pP0) conjugated to a carrier protein has been demonstrated to be effective against the Rhipicephalus microplus, Rhipicephalus sanguineus, and Amblyomma mixtum tick species. The aim of this work was to assess the efficacy of this peptide when conjugated to the Bm86 protein against Dermacentor nitens and Ixodes ricinus ticks. An RNAi experiment using P0 dsRNA from I. ricinus showed a dramatic reduction in the feeding of injected female ticks on guinea pigs. In the follow-up vaccination experiments, rabbits were immunized with the pP0-Bm86 conjugate and challenged simultaneously with larvae, nymphs, and the adults of I. ricinus ticks. In the same way, horses were immunized with the pP0-Bm86 conjugate and challenged with D. nitens larva. The pP0-Bm86 conjugate showed efficacies of 63% and 55% against I. ricinus and D. nitens ticks, respectively. These results, combined with previous reports of efficacy for this conjugate, show the promising potential for its development as a broad-spectrum anti-tick vaccine.<\/jats:p>","DOI":"10.3390\/pathogens12111365","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T09:10:38Z","timestamp":1700212238000},"page":"1365","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Efficacy of the Vaccine Candidate Based on the P0 Peptide against Dermacentor nitens and Ixodes ricinus Ticks"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6950-6793","authenticated-orcid":false,"given":"Alina","family":"Rodr\u00edguez-Mallon","sequence":"first","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, Cuba"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9827-0364","authenticated-orcid":false,"given":"Pedro E.","family":"Encinosa Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, Cuba"}]},{"given":"Yamil","family":"Bello","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, Cuba"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4570-2060","authenticated-orcid":false,"given":"Ana","family":"Domingos","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5512-9093","authenticated-orcid":false,"given":"Sandra","family":"Antunes","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal"}]},{"given":"Petr","family":"Kopacek","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"given":"Ana Sofia","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Vetores e Doen\u00e7as Infeciosas Dr. Francisco Cambournac, Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge (CEVDI-INSA), 2965-575 \u00c1guas de Moura, Portugal"},{"name":"Instituto de Sa\u00fade Ambiental, Faculdade de Medicina, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"given":"Rita","family":"Velez","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Vetores e Doen\u00e7as Infeciosas Dr. Francisco Cambournac, Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge (CEVDI-INSA), 2965-575 \u00c1guas de Moura, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7719-4251","authenticated-orcid":false,"given":"Jan","family":"Perner","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"given":"Frank L.","family":"Ledesma Bravo","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, Cuba"}]},{"given":"Helena","family":"Frantova","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"given":"Jan","family":"Erhart","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech 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