{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:30:30Z","timestamp":1760146230606,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["GHTM-UID\/04413\/2020","LA-REAL\u2013LA\/P\/0117\/2020"],"award-info":[{"award-number":["GHTM-UID\/04413\/2020","LA-REAL\u2013LA\/P\/0117\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Mosquitoes serve as vectors for many arthropod-borne viruses (arboviruses) that are responsible for millions of human infections and thousands of deaths each year. Among these arboviruses, O\u2019nyong-nyong virus (ONNV) is an African alphavirus mainly transmitted by Anopheles mosquitoes. ONNV can be detected through serological or molecular tests, the first showing cross-reactivity to co-circulating alphaviruses and requiring technically demanding confirmation, while the latter, usually based on real-time PCR, are costly and demand specific equipment. Isothermal amplification approaches, such as Loop-Mediated Isothermal Amplification (LAMP), should therefore provide a cost-effective, sensitive, and specific alternative for virus detection, suitable for the resource-limited regions where ONNV circulates up to the present time. Here, we describe the development and optimization of a rapid and highly sensitive (10 pfu\/reaction) RT-LAMP assay for ONNV detection. Additionally, we demonstrate that it is possible to bypass the RNA extraction step, reducing sample handling time and costs. The final RT-LAMPONNV is a promising field detection tool for ONNV, enabling a better understanding of its impact and serving as a point-of-care diagnostic method.<\/jats:p>","DOI":"10.3390\/pathogens13100892","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T11:28:51Z","timestamp":1729078131000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-Sensitivity RT-LAMP for Molecular Detection of O\u2019nyong-nyong (Alphavirus onyong)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-886X","authenticated-orcid":false,"given":"David","family":"Fa\u00edsca-Silva","sequence":"first","affiliation":[{"name":"Institute of Hygiene and Tropical Medicine (IHMT), NOVA University of Lisbon, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}]},{"given":"Gon\u00e7alo","family":"Seixas","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Tropical Medicine (IHMT), NOVA University of Lisbon, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}]},{"given":"M\u00f3nica","family":"Nunes","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"},{"name":"3cE3c-Centre for Ecology, Evolution and Environmental Changes & CHANGE-Global Change and Sustainability Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-9898","authenticated-orcid":false,"given":"Ricardo","family":"Parreira","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Tropical Medicine (IHMT), NOVA University of Lisbon, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Global Health and Tropical Medicine (GHTM), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Associate Lab in Translation and Innovation Towards Global Health, LA-REAL, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s41579-024-01026-0","article-title":"Effects of climate change and human activities on vector-borne diseases","volume":"22","author":"Weaver","year":"2024","journal-title":"Nat. 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