{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:29:19Z","timestamp":1760059759321,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"COPAR Research Group","award":["2025-CP017","2025-CP063"],"award-info":[{"award-number":["2025-CP017","2025-CP063"]}]},{"name":"Deputaci\u00f3n Provincial de Lugo","award":["2025-CP017","2025-CP063"],"award-info":[{"award-number":["2025-CP017","2025-CP063"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Diptera-borne diseases pose a major threat to global health, and their distribution is constantly changing due to climate change, globalization, and environmental changes. To improve the knowledge of dipteran species and their distribution in equine facilities, CDC-UV and oviposition traps were placed, and the dipping technique was performed in 16 equestrian centers of Northwest (NW) Spain (Galicia and Castilla y Le\u00f3n Autonomous Communities) between July and November 2023. A questionnaire was distributed among the horse owners to obtain additional information. Four genera of culicids, Culex (51.8%), Culiseta (38.6%), Anopheles (8.4%), and Aedes\/Ochlerotatus (1.2%) were identified in the equestrian centers. Culex pipiens s.l. was the most prevalent and well-distributed species (93.8% of the centers), whereas Anopheles maculipennis s.l. and An. claviger\/petragnani, the anopheline species, were the most frequent (37.5% and 31.2%, respectively). The Culiseta genus was found in approximately 81.2% of the equine facilities. All genera were collected at medium and high altitudes and in Csb (warm-summer Mediterranean climate) areas. Equestrian centers from NW Spain albeit a variety of culicids with high vectorial capacity, together with an ideal environment for their breeding, the presence of vectors and hosts (humans and animals). This potential problem for global health enhances the need for entomological surveillance.<\/jats:p>","DOI":"10.3390\/pathogens14070661","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T08:58:23Z","timestamp":1751878703000},"page":"661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting the Risk of Diptera-Borne Zoonoses by a Sentinel Equestrian Centers Program"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0368-6645","authenticated-orcid":false,"given":"Cristiana","family":"Cazapal-Monteiro","sequence":"first","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7606-6843","authenticated-orcid":false,"given":"David","family":"Boso","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7361-2347","authenticated-orcid":false,"given":"In\u00eas","family":"Abreu","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0179-7280","authenticated-orcid":false,"given":"Mercedes","family":"Cami\u00f1a","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"given":"Jaime","family":"Sanch\u00eds","sequence":"additional","affiliation":[{"name":"Parasitology and Parasitic Diseases, Faculty of Veterinary, University of \u201cLa Rep\u00fablica\u201d (Regional Litoral Norte), Salto 50000, Uruguay"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0208-3861","authenticated-orcid":false,"given":"Adolfo","family":"Paz-Silva","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6145-7560","authenticated-orcid":false,"given":"Luis","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, and Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1212-8250","authenticated-orcid":false,"given":"Rita","family":"S\u00e1nchez-Andrade","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2682-9015","authenticated-orcid":false,"given":"Mar\u00eda Sol","family":"Arias","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5357-4428","authenticated-orcid":false,"given":"Jos\u00e9 \u00c1ngel","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Control of Parasites Research Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"ref_1","unstructured":"WHO (World Health Organization) (2024, February 21). Vector-Borne Diseases. Factsheet. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/vector-borne-diseases."},{"key":"ref_2","unstructured":"Mullen, G.R., and Durden, L.A. (2018). Mosquitoes (Culicidae). Medical and Veterinary Entomology, Academic Press. [3rd ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1017\/S0007485315000103","article-title":"An entomological review of invasive mosquitoes in Europe","volume":"105","author":"Medlock","year":"2015","journal-title":"Bull. Ent. Res."},{"key":"ref_4","first-page":"124","article-title":"New mosquito traps improve the monitoring of disease vectors","volume":"48","author":"Geier","year":"2006","journal-title":"Int. Pest Control"},{"key":"ref_5","unstructured":"ECDC (European Centre for Disease Prevention and Control) (2024, March 04). Epidemiological Update: West Nile Virus Transmission Season in Europe. Available online: https:\/\/www.ecdc.europa.eu\/en\/news-events\/epidemiological-update-west-nile-virus-transmission-season-europe-2023-0."},{"key":"ref_6","unstructured":"Copernicus ECMWF (European Centre for Medium-Range Weather Forecasts) (2021, April 28). 2020 Warmest Year on Record for Europe; Globally, 2020 Ties with 2016 for Warmest Year Recorded. Available online: https:\/\/climate.copernicus.eu\/copernicus-2020-warmest-year-record-europe-globally-2020-ties-2016-warmest-year-recorded."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s13071-023-05862-6","article-title":"Invasive Aedes mosquitoes in an urban\u2013peri-urban gradient in northern Spain: Evidence of the wide distribution of Aedes japonicus","volume":"16","author":"Cevidanes","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_8","unstructured":"ECDC (European Centre for Disease Prevention and Control) (2024, February 26). Mosquito Maps. Surveillance for Invasive Mosquitoes. Available online: https:\/\/www.ecdc.europa.eu\/en\/disease-vectors\/surveillance-and-disease-data\/mosquito-maps."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s40249-023-01167-z","article-title":"Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization","volume":"12","author":"Brattig","year":"2023","journal-title":"Infect. Dis. Poverty"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.medcli.2018.06.021","article-title":"Arthropods as vectors of transmissible diseases in Spain","volume":"151","author":"Portillo","year":"2018","journal-title":"Med. Clin."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Caballero-G\u00f3mez, J., Cano-Terriza, D., Lecollinet, S., Carbonell, M.D., Mart\u00ednez-Valverde, R., Mart\u00ednez-Nevado, E., Garc\u00eda-P\u00e1rraga, D., Lowensky, S., and Garc\u00eda-Bocanegra, I. (2020). Evidence of exposure to zoonotic flaviviruses in zoo mammals in Spain and their potential role as sentinel species. Vet. Microbiol., 247.","DOI":"10.1016\/j.vetmic.2020.108763"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.medcli.2021.10.014","article-title":"The threat of mosquito-borne arboviral disease in Spain: A bibliographic review","volume":"158","author":"Laycock","year":"2022","journal-title":"Med. Clin."},{"key":"ref_13","unstructured":"Silva, M.I.T. (2021). Epidemiolog\u00eda de D\u00edpteros Que Act\u00faan Como Vectores de Zoonosis en Galicia. Dise\u00f1o de Una Red de Vigilancia de Vectores. [Ph.D. Thesis, Universidade de Santiago de Compostela]. (In Spanish)."},{"key":"ref_14","unstructured":"Polina, A., Pereira, J.M., Mart\u00ednez-Barciela, Y., Pousa, A., \u00cd\u00f1iguez, E., Otero, J.C., and Garrido, J. (2024, March 12). Vixilancia de Mosquitos Vectores de Enfermidades Humanas e Animais: ReGaViVec 2022 Anual Report of Galician Health Services (SERGAS), Xunta de Galicia. Available online: https:\/\/www.sergas.es\/Saude-publica\/Documents\/7149\/2022%20-%20Informe%20Final%20REGAVIVEC%20Mosquitos.pdf."},{"key":"ref_15","first-page":"7854E","article-title":"Surveillance plan proposal for early detection of zoonotic pathogens in Equidae (horses, donkeys)","volume":"20","author":"Carminati","year":"2023","journal-title":"EFSA Support. Publ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S65","DOI":"10.3201\/eid2313.170544","article-title":"Zoonotic disease programs for enhancing global health security","volume":"23","author":"Belay","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"180214","DOI":"10.1038\/sdata.2018.214","article-title":"Present and future K\u00f6ppen-Geiger climate classification maps at 1-km resolution","volume":"5","author":"Beck","year":"2018","journal-title":"Sci. Data"},{"key":"ref_18","unstructured":"Cazapal, C., Abreu, I., Arias, M.S., Paz-Silva, A., and S\u00e1nchez-Andrade, R. (2024, January 18\u201320). Leishmania-spread in Galicia (NW Spain). A canine serological survey. Proceedings of the ALIVE 2 Congress (Animal Leishmaniosis International Veterinary Event), Nice, France."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Becker, N., Petric, D., Zgomba, M., Boase, C., Madon, M.B., Dahl, C., and Kaiser, A. (2010). Mosquitoes: Identification, Ecology and Control, Springer. [3rd ed.].","DOI":"10.1007\/978-3-540-92874-4_16"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ijid.2016.11.277","article-title":"Interactive identification key for female mosquitoes (Diptera: Culicidae) of Euro-Mediterranean and Black Sea regions","volume":"53","author":"Gunay","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3989\/graellsia.1996.v52.i0.376","article-title":"A key, based on wing patterns of biting midges (genus Culicoides latreille-diptera: Ceratopogonidae) in the iberian peninsula, for use in epidemiological studies","volume":"52","author":"Rawlings","year":"1996","journal-title":"Graellsia"},{"key":"ref_22","unstructured":"Polina, A., Pereira, J.M., Mart\u00ednez-Barciela, Y., Pousa, A., Otero, J.C., and Garrido, J. (2024, November 04). Vixilancia de Mosquitos Vectores de Enfermidades Humanas e Animais: ReGaViVec 2021 Anual Report of Galician Health Services (SERGAS), Xunta de Galicia. Available online: https:\/\/www.sergas.es\/Saude-publica\/Documents\/6826\/INFORME_REGAVIVEC_2021.pdf."},{"key":"ref_23","unstructured":"Polina, A., Pereira, J.M., Mart\u00ednez-Barciela, Y., Cobo, F., and Garrido, J. (2024, November 04). Vixilancia de Mosquitos Vectores de Enfermidades Humanas e Animais: ReGaViVec 2023 Anual Report of Galician Health Services (SERGAS), Xunta de Galicia. Available online: https:\/\/www.sergas.es\/Saude-publica\/Documents\/7691\/2023%20-%20Informe%20Final%20REGAVIVEC_def.pdf."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.prevetmed.2015.12.013","article-title":"Diversity and ecology survey of mosquitoes potential vectors in Belgian equestrian farms: A threat prevention of mosquito-borne equine arboviruses","volume":"124","author":"Boukraa","year":"2016","journal-title":"Prev. Vet. Med."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Vogels, C.B.F., M\u00f6hlmann, T.W.R., Melsen, D., Favia, G., Wennergren, U., and Koenraadt, C.J.M. (2016). Latitudinal diversity of Culex pipiens biotypes and hybrids in farm, peri-urban, and wetland habitats in Europe. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0166959"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/jvec.12248","article-title":"The mosquito fauna of the Western region of Spain with emphasis on ecological factors and the characterization of Culex pipiens forms","volume":"42","author":"Gomes","year":"2017","journal-title":"J. Vector Ecol."},{"key":"ref_27","first-page":"91","article-title":"Updated checklist and distribution maps of mosquitoes (Diptera: Culicidae) of Spain","volume":"30","year":"2012","journal-title":"J. EMCA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/s11686-022-00529-1","article-title":"A survey on native and invasive mosquitoes and other biting dipterans in northern Spain","volume":"67","author":"Bengoa","year":"2022","journal-title":"Acta Parasitol."},{"key":"ref_29","unstructured":"Schaffner, F., Angel, G., Geoffroy, B., Hervy, J.P., Rhaiem, A., and Brunhes, J. (2001). The Mosquitoes of Europe, IRD Editions."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jim\u00e9nez-Cabello, L., Utrilla-Trigo, S., Lorenzo, G., Ortego, J., and Calvo-Pinilla, E. (2023). Epizootic hemorrhagic disease virus: Current knowledge and emerging perspectives. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051339"},{"key":"ref_31","unstructured":"WOAH (World Organization for Animal Health) (2024, January 25). Terrestrial Animal Health Code. Available online: https:\/\/www.woah.org\/en\/what-we-do\/standards\/codes-and-manuals\/terrestrial-code-online-access\/?id=169&L=1&htmfile=chapitre_surveillance_vector.htm."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kampen, H., and Werner, D. (2023). Biting midges (Diptera: Ceratopogonidae) as vectors of viruses. Microorganisms, 11.","DOI":"10.3390\/microorganisms11112706"},{"key":"ref_33","unstructured":"L\u00fchken, R. (2014). Mosquitoes and Biting Midges: Data Collection, Identification, Species Distribution, and Breeding Ecology. [Ph.D. Thesis, Carl von Ossietzky University of Oldenburg]. Available online: https:\/\/www.gnatwork.ac.uk\/sites\/gnatwork\/files\/content\/attachments\/2021-04-13\/Dissertation%20L%C3%BChken.pdf."},{"key":"ref_34","first-page":"1","article-title":"Climate change and mosquito-borne diseases in China: A review","volume":"9","author":"Bai","year":"2013","journal-title":"Global Health"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1017\/S0031182022001378","article-title":"Evolutionary consequences of vector-borne transmission: How using vectors shapes host, vector and pathogen evolution","volume":"149","author":"Poulin","year":"2022","journal-title":"Parasitology"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Onyango, M.G., Ciota, A.T., and Kramer, L.D. (2020). The vector-host-pathogen interface: The next frontier in the battle against mosquito-borne viral diseases. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.564518"},{"key":"ref_37","first-page":"38","article-title":"Ecology and diversity of mosquito larvae in ponds and lagoons of Northwestern Spain","volume":"38","author":"Polina","year":"2023","journal-title":"Med. Vet. Entomol."},{"key":"ref_38","first-page":"643","article-title":"Equine Zoonoses: Consequences of Horse-Human Interactions","volume":"Volume 1","author":"Sing","year":"2015","journal-title":"Zoonoses\u2014Infections Affecting Humans and Animals"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/661\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:04:47Z","timestamp":1760033087000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,4]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["pathogens14070661"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14070661","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2025,7,4]]}}}