{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:07:51Z","timestamp":1774321671862,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["FCT\/MCTES (UID\/Multi\/04378\/2019)"],"award-info":[{"award-number":["FCT\/MCTES (UID\/Multi\/04378\/2019)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Recently, studies on the by-products from the food industry, such as passion fruit seeds, have significantly increased, as these can have an added value, due to their properties, such as potential antioxidant activity. This study was conducted to determine the presence of piceatannol and resveratrol in various extracts of passion fruit (Passiflora edulis) seeds from Madeira Island and a commercial passion fruit oil was used as reference. The commercial oil and the extracts that were obtained by traditional Soxhlet method with ethanol and acetone did not reveal the presence of the two stilbenes, piceatannol and resveratrol. However, the extracts that were obtained by the ultrasound method showed significant amounts of piceatannol and resveratrol when compared with the commercial oil. The presence of these compounds indicates that this oil could have potential application in cosmetic and pharmaceutical industries, due to their proven antioxidant and anti-aging properties.<\/jats:p>","DOI":"10.3390\/ph13040073","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T04:49:38Z","timestamp":1587444578000},"page":"73","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Identification and Quantification of Stilbenes (Piceatannol and Resveratrol) in Passiflora edulis By-Products"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9645-4020","authenticated-orcid":false,"given":"Karolline","family":"Krambeck","sequence":"first","affiliation":[{"name":"UCIBIO-REQUIMTE, MedTech, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050 313 Porto, Portugal"}]},{"given":"Ana","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centre for Biotechnology and Fine Chemistry, Faculty of Biotechnology, Catholic University of Portugal, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"given":"Delfim","family":"Santos","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, MedTech, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050 313 Porto, Portugal"}]},{"given":"Maria Manuela","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF\u2013Centre for Biotechnology and Fine Chemistry, Faculty of Biotechnology, Catholic University of Portugal, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Baptista Silva","sequence":"additional","affiliation":[{"name":"Department of Geosciences, University of Aveiro, Campus of Santiago, 3810 193 Aveiro, Portugal"}]},{"given":"Jos\u00e9 Manuel","family":"Sousa Lobo","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, MedTech, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050 313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3209-3366","authenticated-orcid":false,"given":"Maria Helena","family":"Amaral","sequence":"additional","affiliation":[{"name":"UCIBIO-REQUIMTE, MedTech, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050 313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"164","DOI":"10.5923\/j.fph.20150505.03","article-title":"Trends for the Application of Passion Fruit Industrial By-products: A Review on the Chemical Composition and Extraction Techniques of Phytochemicals","volume":"5","author":"Martinez","year":"2015","journal-title":"Food Public Heal."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lucarini, M., Durazzo, A., Raffo, A., Giovannini, A., and Kiefer, J. (2019). Passion Fruit (Passiflora spp.) Seed Oil. Fruit Oils: Chemistry and Functionality, Springer.","DOI":"10.1007\/978-3-030-12473-1_29"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"De Toledo, N.M.V., De Camargo, A.C., Ramos, P.B.M., Button, D.C., Granato, D., and Canniatti-Brazaca, S.G. (2018). Potentials and pitfalls on the use of passion fruit by-products in drinkable yogurt: Physicochemical, technological, microbiological, and sensory aspects. Beverages, 4.","DOI":"10.3390\/beverages4030047"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"121983","DOI":"10.1016\/j.cej.2019.121983","article-title":"Value-added chemicals from food supply chain wastes: State-of-the-art review and future prospects","volume":"375","author":"Xiong","year":"2019","journal-title":"Chem. Eng. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"135747","DOI":"10.1016\/j.scitotenv.2019.135747","article-title":"Valorization of marine-derived biowaste to develop chitin\/fish gelatin products as bioactive carriers and moisture scavengers","volume":"706","author":"Uranga","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s12393-019-09206-y","article-title":"Green Solvents for the Extraction of High Added-Value Compounds from Agri-food Waste","volume":"12","author":"Rubio","year":"2020","journal-title":"Food Eng. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jfoodeng.2012.12.004","article-title":"The extraction of passion fruit oil with green solvents","volume":"117","author":"Gimenes","year":"2013","journal-title":"J. Food Eng."},{"key":"ref_8","first-page":"209","article-title":"Optimization of extraction parameters on the antioxidant activity of passion fruit waste","volume":"6","author":"Krambeck","year":"2018","journal-title":"Acad. J. Med. Plants"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.phrs.2017.08.002","article-title":"Anti-inflammatory activity of natural stilbenoids: A review","volume":"124","author":"Dvorakova","year":"2017","journal-title":"Pharmacol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"11112","DOI":"10.1021\/jf102650d","article-title":"Extract of passion fruit (Passiflora edulis) seed containing high amounts of piceatannol inhibits melanogenesis and promotes collagen synthesis","volume":"58","author":"Matsui","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Salehi, B., Mishra, A.P., Nigam, M., Sener, B., Kilic, M., Sharifi-Rad, M., Fokou, P.V.T., Martins, N., and Sharifi-Rad, J. (2018). Resveratrol: A double-edged sword in health benefits. Biomedicines, 6.","DOI":"10.3390\/biomedicines6030091"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.etap.2009.05.013","article-title":"Resveratrol ameliorates carbon tetrachloride-induced acute liver injury in mice","volume":"28","author":"Fan","year":"2009","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ko, J.H., Sethi, G., Um, J.Y., Shanmugam, M.K., Arfuso, F., Kumar, A.P., Bishayee, A., and Ahn, K.S. (2017). The role of resveratrol in cancer therapy. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18122589"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8976","DOI":"10.1021\/cr500689b","article-title":"Chemistry and Biology of Resveratrol-Derived Natural Products","volume":"115","author":"Keylor","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Nawaz, W., Zhou, Z., Deng, S., Ma, X., Ma, X., Li, C., and Shu, X. (2017). Therapeutic Versatility of Resveratrol Derivatives. Nutrients, 9.","DOI":"10.3390\/nu9111188"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"124","DOI":"10.7324\/JAPS.2019.90717","article-title":"Resveratrol and pterostilbene: A comparative overview of their chemistry, biosynthesis, plant sources and pharmacological properties","volume":"9","author":"Chan","year":"2019","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Aires, V., Colin, D.J., Doreau, A., Di Pietro, A., Heydel, J.M., Artur, Y., Latruffe, N., and Delmas, D. (2019). P-glycoprotein 1 affects chemoactivities of resveratrol against human colorectal cancer cells. Nutrients, 11.","DOI":"10.3390\/nu11092098"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/molecules22060885","article-title":"Cellular and molecular targets of resveratrol on lymphoma and leukemia cells","volume":"22","author":"Frazzi","year":"2017","journal-title":"Molecules"},{"key":"ref_19","unstructured":"Levenson, A.S. (2020). Metastasis-associated protein 1-mediated antitumor and anticancer activity of dietary stilbenes for prostate cancer chemoprevention and therapy. Semin. Cancer Biol., in press."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4952","DOI":"10.1021\/acs.jafc.7b01254","article-title":"Antioxidant and Cytoprotective Activities of Grapevine Stilbenes","volume":"65","author":"Biais","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.mrrev.2011.11.001","article-title":"Biological activity of piceatannol: Leaving the shadow of resveratrol","volume":"750","author":"Piotrowska","year":"2012","journal-title":"Mutat. Res. Rev. Mutat. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s00217-017-3007-y","article-title":"Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales","volume":"244","author":"Vasdekis","year":"2018","journal-title":"Eur. Food Res. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Akinwumi, B.C., Bordun, K.A.M., and Anderson, H.D. (2018). Biological activities of stilbenoids. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030792"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3177\/jnsv.64.75","article-title":"Effect of passion fruit seed extract rich in piceatannol on the skin of women: A randomized, placebo-controlled, double-blind trial","volume":"64","author":"Morita","year":"2018","journal-title":"J. Nutr. Sci. Vitaminol. (Tokyo)."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1248\/bpb.30.2007","article-title":"Piceatannol Inhibits Melanogenesis by Its Antioxidative Actions","volume":"30","author":"Yokozawa","year":"2007","journal-title":"Biol. Pharm. Bull."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.indcrop.2017.10.011","article-title":"Assessment of alcoholic distillates for the extraction of bioactive polyphenols from grapevine canes","volume":"111","author":"Ramil","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.foodres.2016.04.015","article-title":"Sequential high pressure extractions applied to recover piceatannol and scirpusin B from passion fruit bagasse","volume":"85","author":"Aguiar","year":"2016","journal-title":"Food Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1021\/jf9909196","article-title":"Isolation and Identification of Stilbenes in Two Varieties of Polygonum cuspidatum","volume":"48","author":"Vastano","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1021\/jf050242o","article-title":"Production of Stilbenoids from the Callus of Arachis hypogaea:\u2009 A Novel Source of the Anticancer Compound Piceatannol","volume":"53","author":"Ku","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1002\/jssc.200800119","article-title":"Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with CoulArray detection","volume":"31","author":"Adam","year":"2008","journal-title":"J. Sep. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1021\/jf020939z","article-title":"Postharvest UV-C-Irradiated Grapes as a Potential Source for Producing Stilbene-Enriched Red Wines","volume":"51","author":"Cantos","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cep.2012.01.003","article-title":"Intensification of extraction of natural products using ultrasonic irradiations\u2014A review of current status","volume":"53","author":"Shirsath","year":"2012","journal-title":"Chem. Eng. Process. Process Intensif."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.foodchem.2007.06.062","article-title":"Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran","volume":"106","author":"Wang","year":"2008","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.tifs.2005.12.004","article-title":"Recent advances in extraction of nutraceuticals from plants","volume":"17","author":"Wang","year":"2006","journal-title":"Trends Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12","DOI":"10.5923\/j.fph.20130301.02","article-title":"Extraction of Polyphenols and Anthocyanins from the Jambul (Syzygium cumini) Fruit Peels","volume":"3","author":"Santos","year":"2013","journal-title":"Food Public Heal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.foodres.2018.04.006","article-title":"UHPLC-QqQ-MS\/MS identification, quantification of polyphenols from Passiflora subpeltata fruit pulp and determination of nutritional, antioxidant, \u03b1-amylase and \u03b1-glucosidase key enzymes inhibition properties","volume":"108","author":"Shanmugam","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1021\/jf040095e","article-title":"Resveratrol, pterostilbene, and piceatannol in Vaccinium berries","volume":"52","author":"Rimando","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1016\/j.foodchem.2012.10.125","article-title":"Piceatannol, a potent bioactive stilbene, as major phenolic component in Rhodomyrtus tomentosa","volume":"138","author":"Lai","year":"2013","journal-title":"Food Chem."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/13\/4\/73\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:45:13Z","timestamp":1760363113000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/13\/4\/73"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":38,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["ph13040073"],"URL":"https:\/\/doi.org\/10.3390\/ph13040073","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,20]]}}}