{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T19:00:22Z","timestamp":1774119622207,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,12,17]],"date-time":"2023-12-17T00:00:00Z","timestamp":1702771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>The evaluation of the efficacy of incorporation of quercetin in nanoparticles is crucial, both for the development and quality control of pharmaceutical formulations. The validation of analytical methods for the precise quantification of quercetin is useful for the evaluation of various potential quercetin delivery systems and quercetin pharmacokinetics. This work aimed to validate a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for quercetin detection and quantification in nanoparticles. Different mobile phase conditions and detection wavelengths (254 and 368 nm) were tested, and the major validation parameters were assessed (precision, accuracy, linearity, sensitivity, stability, and selectivity). The best peak resolution was obtained when quercetin was analyzed at 368 nm with a mobile phase of 1.5% acetic acid and a water\/acetonitrile\/methanol ratio of 55:40:5. Under these conditions, quercetin also eluted rapidly (retention time of 3.6 min). The method proved to be linear (R2 &gt; 0.995), specific, and repeatable (variation coefficient between 2.4% and 6.7%) and presented intermediate precision (variation coefficient between 7.2% and 9.4%). The accuracy of the analysis ranged between 88.6% and 110.7%, and detection and quantification limits were 0.046 and 0.14 \u00b5g\/mL, respectively. Quercetin solutions were more stable when stored at 4 \u00b0C than at room temperature or \u221220 \u00b0C. This validated method satisfied more parameters of bias assessment than most recent methods for quercetin determination and presented itself as more sensitive and efficient than general spectrophotometric methods. The method was successfully used for the analysis of quercetin incorporation in nanoparticles and will be evaluated in the future for its adequacy for the determination of quercetin in more complex matrices.<\/jats:p>","DOI":"10.3390\/ph16121736","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T03:59:24Z","timestamp":1702871964000},"page":"1736","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Validation of an HPLC-DAD Method for Quercetin Quantification in Nanoparticles"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3058-2715","authenticated-orcid":false,"given":"Daniel","family":"Carvalho","sequence":"first","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3972-8253","authenticated-orcid":false,"given":"\u00c2ngelo","family":"Jesus","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"REQUIMTE-LAQV, Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3157-3712","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Pinho","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"REQUIMTE-LAQV, Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-5740","authenticated-orcid":false,"given":"Rita Ferraz","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"REQUIMTE-LAQV, Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3452-1459","authenticated-orcid":false,"given":"Fernando","family":"Moreira","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"REQUIMTE-LAQV, Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4420-6476","authenticated-orcid":false,"given":"Ana Isabel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"REQUIMTE-LAQV, Escola Superior de Sa\u00fade, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e06553","DOI":"10.1016\/j.heliyon.2021.e06553","article-title":"Choices of Chromatographic Methods as Stability Indicating Assays for Pharmaceutical Products: A Review","volume":"7","author":"Chew","year":"2021","journal-title":"Heliyon"},{"key":"ref_2","unstructured":"Skoog, D.A., Holler, F.J., and Crouch, S.R. (2018). Principles of Instrumental Analysis, Cengage Learning. [7th ed.]."},{"key":"ref_3","unstructured":"Harris, D.C. (2010). Quantitative Chemical Analysis, W. H. Freeman and Company. [8th ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1016\/j.jchromb.2008.09.030","article-title":"Key Aspects of Analytical Method Validation and Linearity Evaluation","volume":"877","author":"Araujo","year":"2009","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Stauffer, M.T. (2018). Calibration and Validation of Analytical Methods\u2014A Sampling of Current Approaches, InTech.","DOI":"10.5772\/intechopen.69918"},{"key":"ref_6","unstructured":"International Conference on Harmonization (2022). Validation of Analytical Procedures: Q2(R2), International Conference on Harmonization."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xu, D., Hu, M.-J., Wang, Y.-Q., and Cui, Y.-L. (2019). Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application. Molecules, 24.","DOI":"10.3390\/molecules24061123"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Li, Y., Yao, J., Han, C., Yang, J., Chaudhry, M.T., Wang, S., Liu, H., and Yin, Y. (2016). Quercetin, Inflammation and Immunity. Nutrients, 8.","DOI":"10.3390\/nu8030167"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Tang, J., Diao, P., Shu, X., Li, L., and Xiong, L. (2019). Quercetin and Quercitrin Attenuates the Inflammatory Response and Oxidative Stress in LPS-Induced RAW264.7 Cells: In vitro Assessment and a Theoretical Model. BioMed Res. Int., 2019.","DOI":"10.1155\/2019\/7039802"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Hussain, Y., Mirzaei, S., Ashrafizadeh, M., Zarrabi, A., Hushmandi, K., Khan, H., and Daglia, M. (2021). Quercetin and Its Nano-Scale Delivery Systems in Prostate Cancer Therapy: Paving the Way for Cancer Elimination and Reversing Chemoresistance. Cancers, 13.","DOI":"10.3390\/cancers13071602"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e11159","DOI":"10.1016\/j.heliyon.2022.e11159","article-title":"Amelioration of Ethanol-Induced Gastric Ulcer in Rats by Quercetin: Implication of Nrf2\/HO1 and HMGB1\/TLR4\/NF-\u039aB Pathways","volume":"8","author":"Shams","year":"2022","journal-title":"Heliyon"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.fitote.2015.09.018","article-title":"Quercetin: A Flavonol with Multifaceted Therapeutic Applications?","volume":"106","year":"2015","journal-title":"Fitoterapia"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pinheiro, R.G.R., Pinheiro, M., and Neves, A.R. (2021). Nanotechnology Innovations to Enhance the Therapeutic Efficacy of Quercetin. Nanomaterials, 11.","DOI":"10.3390\/nano11102658"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"902092","DOI":"10.3389\/fnagi.2022.902092","article-title":"Network Pharmacology Identify Intersection Genes of Quercetin and Alzheimer\u2019s Disease as Potential Therapeutic Targets","volume":"14","author":"Wei","year":"2022","journal-title":"Front. Aging Neurosci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tifs.2016.07.004","article-title":"The Biological Activities, Chemical Stability, Metabolism and Delivery Systems of Quercetin: A Review","volume":"56","author":"Wang","year":"2016","journal-title":"Trends Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"114068","DOI":"10.1016\/j.ejmech.2021.114068","article-title":"Quercetin Derivatives: Drug Design, Development, and Biological Activities, a Review","volume":"229","author":"Alizadeh","year":"2022","journal-title":"Eur. J. Med. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"727","DOI":"10.2174\/1381612828666220317141923","article-title":"Research Progress of Quercetin Delivery Systems","volume":"28","author":"Zhao","year":"2022","journal-title":"Curr. Pharm. Des."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"109233","DOI":"10.1016\/j.microc.2023.109233","article-title":"Sample Preparation Methods for Determination of Quercetin and Quercetin Glycosides in Diverse Matrices","volume":"194","author":"Hamed","year":"2023","journal-title":"Microchem. J."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mansour, F.R., Abdallah, I.A., Bedair, A., and Hamed, M. (2023). Analytical Methods for the Determination of Quercetin and Quercetin Glycosides in Pharmaceuticals and Biological Samples. Crit. Rev. Anal. Chem., 1\u201326.","DOI":"10.1080\/10408347.2023.2269421"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Carvalho, D., Pinho, C., Oliveira, R., Moreira, F., and Oliveira, A.I. (2023). Chromatographic Methods Developed for the Quantification of Quercetin Extracted from Natural Sources: Systematic Review of Published Studies from 2018 to 2022. Molecules, 28.","DOI":"10.3390\/molecules28237714"},{"key":"ref_21","first-page":"S37","article-title":"Assessment of Bias with Emphasis on Method Comparison","volume":"29","author":"Johnson","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Abdelkawy, K.S., Balyshev, M.E., and Elbarbry, F. (2017). A New Validated HPLC Method for the Determination of Quercetin: Application to Study Pharmacokinetics in Rats. Biomed. Chromatogr., 31.","DOI":"10.1002\/bmc.3819"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"462312","DOI":"10.1016\/j.chroma.2021.462312","article-title":"Development and Validation of a Liquid Chromatographic Method with Diode Array Detection for the Determination of Anthraquinones, Flavonoids and Other Natural Dyes in Aged Silk","volume":"1651","author":"Vasileiadou","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.jpba.2005.04.052","article-title":"Simultaneous Determination of Catechin, Rutin, Quercetin, Kaempferol and Isorhamnetin in the Extract of Sea Buckthorn (Hippophae rhamnoides L.) Leaves by RP-HPLC with DAD","volume":"41","author":"Zu","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1140\/epje\/i2009-10485-7","article-title":"The UV-Vis Absorption Spectrum of the Flavonol Quercetin in Methanolic Solution: A Theoretical Investigation","volume":"29","author":"Ribeiro","year":"2009","journal-title":"Eur. Phys. J. E"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"De, R., Jo, K.W., and Kim, K.-T. (2022). Influence of Molecular Structures on Fluorescence of Flavonoids and Their Detection in Mammalian Cells. Biomedicines, 10.","DOI":"10.3390\/biomedicines10061265"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.jpba.2004.12.022","article-title":"Chromatographic and Electrochemical Determination of Quercetin and Kaempferol in Phytopharmaceuticals","volume":"38","year":"2005","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3831\/KPI.2014.17.035","article-title":"HPLC Method for Simultaneous Quantitative Detection of Quercetin and Curcuminoids in Traditional Chinese Medicines","volume":"17","author":"Ang","year":"2014","journal-title":"J. Pharmacopunct."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1055\/s-0030-1249878","article-title":"Cotinus coggygria Wood: Novel Flavanone Dimer and Development of an HPLC\/UV\/MS Method for the Simultaneous Determination of Fourteen Phenolic Constituents","volume":"76","author":"Antal","year":"2010","journal-title":"Planta Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1093\/jaoacint\/qsab024","article-title":"Dronedarone HCl-Quercetin Co-Amorphous System: Characterization and RP-HPLC Method Development for Simultaneous Estimation","volume":"104","author":"Sree","year":"2021","journal-title":"J. AOAC Int."},{"key":"ref_31","first-page":"25","article-title":"A Validated Chromatographic Method for the Determination of Flavonoids in Copaifera langsdorffii by HPLC","volume":"7","author":"Brancalion","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_32","unstructured":"Moosavi, S.M., and Ghassabian, S. (2018). Calibration and Validation of Analytical Methods\u2014A Sampling of Current Approaches, InTechOpen."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1002\/jssc.201200287","article-title":"New Validated High-Performance Liquid Chromatographic Method for Simultaneous Analysis of Ten Flavonoid Aglycones in Plant Extracts Using a C18 Fused-Core Column and Acetonitrile-Tetrahydrofuran Gradient","volume":"35","author":"Olszewska","year":"2012","journal-title":"J. Sep. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5226","DOI":"10.1021\/jf034149g","article-title":"Direct HPLC Analysis of Quercetin and Trans-Resveratrol in Red Wine, Grape, and Winemaking Byproducts","volume":"51","author":"Careri","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.jpba.2009.11.004","article-title":"Simultaneous Determination of Eleven Bioactive Compounds in Saururus chinensis from Different Harvesting Seasons by HPLC-DAD","volume":"51","author":"Chen","year":"2010","journal-title":"J. Pharm. Biomed Anal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"14241","DOI":"10.3390\/molecules181114241","article-title":"Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection","volume":"18","author":"Zhang","year":"2013","journal-title":"Molecules"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"263","DOI":"10.4103\/0973-1296.103649","article-title":"Determination of Quercetin, Plumbagin and Total Flavonoids in Drosera peltata Smith var. glabrata Y.Z.Ruan","volume":"8","author":"He","year":"2012","journal-title":"Pharmacogn. Mag."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.trac.2007.01.009","article-title":"A Practical Guide to Analytical Method Validation, Including Measurement Uncertainty and Accuracy Profiles","volume":"26","author":"Herrador","year":"2007","journal-title":"TrAC\u2014Trends Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"173172","DOI":"10.1016\/j.ejphar.2020.173172","article-title":"Quercetin Loaded Chitosan Tripolyphosphate Nanoparticles Accelerated Cutaneous Wound Healing in Wistar Rats","volume":"880","author":"Choudhary","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1590\/S0100-40422004000500017","article-title":"Valida\u00e7\u00e3o Em M\u00e9todos Cromatogr\u00e1ficos e Eletrofor\u00e9ticos","volume":"27","author":"Ribani","year":"2004","journal-title":"Qu\u00edm. Nova"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"518175","DOI":"10.1155\/2014\/518175","article-title":"Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS\/MS","volume":"2014","author":"Satheeshkumar","year":"2014","journal-title":"Chromatogr. Res. Int."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Dabeek, W.M., and Marra, M.V. (2019). Dietary Quercetin and Kaempferol: Bioavailability and Potential Cardiovascular-Related Bioactivity in Humans. Nutrients, 11.","DOI":"10.3390\/nu11102288"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1007\/s13197-021-05312-0","article-title":"Development and Validation of a Reverse Phase HPLC-DAD Method for Separation, Detection & Quantification of Rutin and Quercetin in Buckwheat (Fagopyrum spp.)","volume":"59","author":"Jan","year":"2022","journal-title":"J. Food Sci. Technol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1080\/03639045.2021.1892740","article-title":"Analytical Method Development, Validation and Forced Degradation Studies for Rutin, Quercetin, Curcumin, and Piperine by RP-UFLC Method","volume":"47","author":"Ramaswamy","year":"2021","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.jpba.2019.06.044","article-title":"Simultaneous Quantification of Five Bioactive Phenylethanoid, Iridoid, and Flavonol Glycosides in Duranta erecta L.: Ultra Performance Liquid Chromatography Method Validation and Uncertainty Measurement","volume":"174","author":"Srivastava","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.sjbs.2020.09.056","article-title":"Quantification of Biochemical Compounds in Bauhinia variegata Linn Flower Extract and Its Hepatoprotective Effect","volume":"28","author":"Gul","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_47","first-page":"2692","article-title":"A New Method in Some German Grape Wines Using Zic-Hilic Technology with UV Detection to Separate and Identify Quercetin","volume":"20","author":"Karabat","year":"2020","journal-title":"Plant Arch."},{"key":"ref_48","first-page":"441","article-title":"Quantitative Determination of Quercetin in Ginkgo biloba Leaf Extract (EGb 761) Using GC-MS","volume":"16","author":"Abdulrazak","year":"2017","journal-title":"Nigerian J. Sci. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.imu.2018.10.004","article-title":"Chromatographic Analysis of Phytochemicals in Costus igneus and Computational Studies of Flavonoids","volume":"13","author":"Peasari","year":"2018","journal-title":"Inform. Med. Unlocked"},{"key":"ref_50","unstructured":"Desmiaty, Y., and Alatas, F. (2008, January 30\u201331). Determination of Quercetin in Hibiscus sabdariffa L. Calyces by High-Performance Liquid Chromatography (HPLC). In Proceeding of the International Seminar on Chemistry, West Java, Indonesia."},{"key":"ref_51","first-page":"534","article-title":"Simultaneous Determination of Phenolic Compounds in Brassica oleracea L.","volume":"6","author":"Ahmed","year":"2014","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.chroma.2004.01.071","article-title":"Measurement and Simulation of Tailing Zones of a Cationic Dye in Analytical-Scale Reversed Phase Chromatography","volume":"1034","author":"Wirth","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Khan, M.N., Ul Haq, F., Rahman, S., Ali, A., and Musharraf, S.G. (2020). Metabolite Distribution and Correlation Studies of Ziziphus jujuba and Ziziphus nummularia Using LC-ESI-MS\/MS. J. Pharm. Biomed. Anal., 178.","DOI":"10.1016\/j.jpba.2019.112918"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Jia, Q., Zhang, S., Zhang, H., Yang, X., Cui, X., Su, Z., and Hu, P. (2020). A Comparative Study on Polyphenolic Composition of Berries from the Tibetan Plateau by UPLC-Q-Orbitrap MS System. Chem. Biodivers., 17.","DOI":"10.1002\/cbdv.202000033"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/pca.2899","article-title":"Quantitative Analysis of Flavonols, Flavonol Glycoside and Homoisoflavonoids in Polygonatum verticillatum Using UHPLC-DAD-QTOF-IMS and Evaluation of Their Antioxidant Potential","volume":"31","author":"Sharma","year":"2020","journal-title":"Phytochem. Anal."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"389","DOI":"10.30574\/wjarr.2022.16.2.1165","article-title":"Analytical Method Validation: A Brief Review","volume":"16","author":"Chavan","year":"2022","journal-title":"World J. Adv. Res. Rev."},{"key":"ref_57","first-page":"17","article-title":"Separation and Determination of Selected Polyphenols from Medicinal Plants","volume":"57","author":"Hejniak","year":"2019","journal-title":"J. Chromatogr. Sci."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/16\/12\/1736\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:40:15Z","timestamp":1760132415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/16\/12\/1736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,17]]},"references-count":57,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["ph16121736"],"URL":"https:\/\/doi.org\/10.3390\/ph16121736","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,17]]}}}