{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:25:03Z","timestamp":1760145903277,"version":"build-2065373602"},"reference-count":17,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"scope of the CICS-UBI","award":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"],"award-info":[{"award-number":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"]}]},{"name":"Portuguese Foundation for Science and Technology\/MCTES","award":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"],"award-info":[{"award-number":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"]}]},{"name":"FCT fellowship","award":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"],"award-info":[{"award-number":["UIDB\/00709\/2020","UIDP\/00709\/2020","SFRH\/BD146395\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Human papillomavirus (HPV), a leading cause of cervical cancer, is present in most cases of the disease and ranks as the fourth most common cancer in women globally. Among the HPV types, fourteen (HPV 16\/18\/31\/33\/35\/39\/45\/51\/52\/56\/58\/59\/66\/68) are recognized as high-risk (hrHPV), each with varying levels of oncogenic potential. Detecting and genotyping these hrHPV types in cervical lesions is crucial, requiring the development of new diagnostic methods. Methods: This study focuses on a retrospective analysis conducted on 44 women from the Cova da Beira Local Health Unit. We used the Anyplex\u2122 II hrHPV Detection kit for hrHPV genotyping from paraffin-embedded cervical tissue samples. Results: hrHPV types were identified in 38 out of the 44 women. Genotyping revealed HPV-16 (55.3%), HPV-18\/39\/56\/58\/59 (5.3%), HPV-31 (21.1%), HPV-35 (7.9%), HPV-51\/66 (2.6%), and HPV-52 (10.5%). Conclusions: This study demonstrates that the Anyplex\u2122 II hrHPV Detection kit, originally designed for cervical cancer screening, is also effective for hrHPV genotyping in histological analyses. This methodology offers a simpler and more cost-effective approach for cervical cancer risk stratification. Its implementation in clinical practice could enhance the detection of hrHPV in cervical lesions, thereby contributing to more precise diagnoses and potentially more informed treatment strategies.<\/jats:p>","DOI":"10.3390\/ph17091201","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T05:03:04Z","timestamp":1726117384000},"page":"1201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High-Risk HPV Detection in Paraffin-Embedded Tissue from Cervical Lesions"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4280-0765","authenticated-orcid":false,"given":"Micaela","family":"Almeida","sequence":"first","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Department of Pathology, Ipatimup Diagnostics, Rua J\u00falio Amaral de Carvalho, 45, 4200-135 Porto, Portugal"},{"name":"I3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, University of Porto, 4200-135 Porto, Portugal"},{"name":"Clinical Academic Centre of Beiras (CACB), Edif\u00edcio UBImedical, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1333-9404","authenticated-orcid":false,"given":"Vitor","family":"Caeiro","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Cova da Beira Local Health Unit, Alameda P\u00earo da Covilh\u00e3, 6200-251 Covilh\u00e3, Portugal"}]},{"given":"Diana","family":"Costa","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"given":"Lara","family":"Silva","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"given":"C\u00edntia","family":"Sousa","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"given":"Paula","family":"Pestana","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Clinical Academic Centre of Beiras (CACB), Edif\u00edcio UBImedical, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"},{"name":"Cova da Beira Local Health Unit, Alameda P\u00earo da Covilh\u00e3, 6200-251 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6847-5630","authenticated-orcid":false,"given":"Sofia","family":"Campelos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ipatimup Diagnostics, Rua J\u00falio Amaral de Carvalho, 45, 4200-135 Porto, Portugal"},{"name":"I3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, University of Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7397-0839","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Vale","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ipatimup Diagnostics, Rua J\u00falio Amaral de Carvalho, 45, 4200-135 Porto, Portugal"},{"name":"I3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, University of Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4783-665X","authenticated-orcid":false,"given":"Ana Cristina","family":"Ramalhinho","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Clinical Academic Centre of Beiras (CACB), Edif\u00edcio UBImedical, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"},{"name":"Cova da Beira Local Health Unit, Alameda P\u00earo da Covilh\u00e3, 6200-251 Covilh\u00e3, Portugal"}]},{"given":"Jos\u00e9","family":"Fonseca-Moutinho","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Clinical Academic Centre of Beiras (CACB), Edif\u00edcio UBImedical, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"},{"name":"Cova da Beira Local Health Unit, Alameda P\u00earo da Covilh\u00e3, 6200-251 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8550-1645","authenticated-orcid":false,"given":"Luiza","family":"Breitenfeld","sequence":"additional","affiliation":[{"name":"Health Sciences Research Centre (CICS), Faculty of Health Sciences, University of Beira Interior (UBI), Avenida Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Clinical Academic Centre of Beiras (CACB), Edif\u00edcio UBImedical, Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1177\/1352458518807088","article-title":"Warts and all: Fingolimod and unusual HPV-associated lesions","volume":"25","author":"Triplett","year":"2019","journal-title":"Mult. Scler."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1470-2045(09)70096-8","article-title":"A review of human carcinogens\u2014Part B: Biological agents","volume":"10","author":"Bouvard","year":"2009","journal-title":"Lancet Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e191","DOI":"10.1016\/S2214-109X(19)30482-6","article-title":"Estimates of incidence and mortality of cervical cancer in 2018: A worldwide analysis","volume":"8","author":"Arbyn","year":"2020","journal-title":"Lancet Glob. Health"},{"key":"ref_5","unstructured":"World Health Organization (2021). WHO Guideline for Screening and Treatment of Cervical Pre-Cancer Lesions for Cervical Cancer Prevention, World Health Organization. [2nd ed.]. Licence: CC BY-NC-SA 3.0 IGO."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1089\/jwh.2020.8918","article-title":"Summary of current guidelines for cervical cancer screening and management of abnormal test results: 2016\u20132020","volume":"30","author":"Perkins","year":"2021","journal-title":"J. Womens Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1080\/08941939.2020.1850944","article-title":"Factors correlated with the accuracy of colposcopy-directed biopsy: A systematic review and meta-analysis","volume":"35","author":"Ren","year":"2022","journal-title":"J. Investig. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1097\/LGT.0000000000000525","article-title":"ASCCP risk-based management consensus guidelines for abnormal cervical cancer screening tests and cancer precursors","volume":"24","author":"Perkins","year":"2020","journal-title":"J. Low. Genit. Tract. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3322\/caac.21628","article-title":"Cervical cancer screening for individuals at average risk: 2020 guideline update from the American Cancer Society","volume":"70","author":"Fontham","year":"2020","journal-title":"CA Cancer J. Clin."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"605","DOI":"10.2174\/1386207323666200901101955","article-title":"Cervical cancer diagnosis: Insights into biochemical biomarkers and Imaging techniques","volume":"24","author":"Tavakoli","year":"2021","journal-title":"Comb. Chem. High. Throughput Screen."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1097\/IGC.0b013e31821dd3b2","article-title":"Prevalence of humean papillomavirus in infection in women in Portugal: The CLEOPATRE Portugal study","volume":"21","author":"Pista","year":"2011","journal-title":"Int. J. Gynecol. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1080\/0284186X.2023.2264485","article-title":"Prevalente of human papillomavirus (HPV) types 16 and 18 in cervical cancer in Stockholm, Sweden during 2019\u20132023 compared to 2003\u20132008","volume":"62","author":"Silvars","year":"2023","journal-title":"Acta Oncol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.4103\/joc.joc_42_22","article-title":"The prevalence of cervical HPV infection and genotype distribution in 856,535 Chinese women with normal and abnormal cervical lesions: A systemic review","volume":"39","author":"Yu","year":"2022","journal-title":"J. Cytol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Temesgen, M.M., Alemu, T., Shiferaw, B., Legesse, S., Zeru, T., Haile, M., and Gelanew, T. (2021). Prevalence of oncogenic human papillomavirus (HPV 16\/18) infection, cervical lesions and its associated factors among women aged 21\u201349 years in Amhara region, Northern Ethiopia. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0248949"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1086\/657321","article-title":"Cervical human papillomavirus prevalence in 5 continents: Meta-analysis of 1 million women with normal cytological findings","volume":"202","author":"Bruni","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100179","DOI":"10.1016\/j.pvr.2019.100179","article-title":"High-Risk human papillomavirus genotype distribution in the Northern region of Portugal: Data from regional cervical cancer screening program","volume":"8","author":"Sousa","year":"2019","journal-title":"Papillomavirus Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jcv.2016.01.009","article-title":"Clinical validation of Anyplex\u2122 II HPV HR Detection according to the guidelines for HPV test requirements for cervical cancer screening","volume":"76","author":"Hesselink","year":"2016","journal-title":"J. Clin. Virol."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/9\/1201\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:54:25Z","timestamp":1760111665000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/17\/9\/1201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":17,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["ph17091201"],"URL":"https:\/\/doi.org\/10.3390\/ph17091201","relation":{},"ISSN":["1424-8247"],"issn-type":[{"type":"electronic","value":"1424-8247"}],"subject":[],"published":{"date-parts":[[2024,9,12]]}}}