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Among them, four species stand out due to their traditional uses: Equisetum ramosissimum Desf. (hemorrhages, urethritis, hepatitis), Rumex scutatus L. subsp. induratus (Boiss. and Reut.) Malag. (inflammation, constipation), Geranium purpureum Vill., and Geranium lucidum L. (pain relief, gastric issues). Given their rich ethnomedicinal history, we evaluated their protective effects on an in vitro model of metabolic dysfunction-associated steatotic liver disease (MASLD). Methods: Decoction (D) and hydroalcoholic (EtOH80%) extracts were prepared and chemically characterized. Their safety profile and effects on lipid accumulation were assessed in palmitic acid (PA)-treated HepG2 cells using resazurin, sulforhodamine B, and Nile Red assays. Results: Chemical analysis revealed diverse phenolic compounds, particularly kaempferol derivatives in E. ramosissimum. All extracts showed minimal cytotoxicity at 25\u201350 \u00b5g\/mL. At 100 \u00b5g\/mL, only E. ramosissimum extracts maintained high cell viability. In the lipotoxicity model, E. ramosissimum decoction demonstrated the most potent effect, significantly reducing PA-induced neutral lipid accumulation in a dose-dependent manner, while other extracts showed varying degrees of activity. Conclusions: These findings highlight E. ramosissimum\u2019s decoction, rich in kaempferol derivatives, as particularly effective in reducing lipid accumulation in this MASLD cell model while also providing a comprehensive characterization of traditionally used plants from the C\u00f4a Valley region.<\/jats:p>","DOI":"10.3390\/ph18010039","type":"journal-article","created":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T11:24:19Z","timestamp":1735730659000},"page":"39","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chemical Characterization and Differential Lipid-Modulating Effects of Selected Plant Extracts from C\u00f4a Valley (Portugal) in a Cell Model for Liver Steatosis"],"prefix":"10.3390","volume":"18","author":[{"given":"Ricardo","family":"Amorim","sequence":"first","affiliation":[{"name":"Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5414-366X","authenticated-orcid":false,"given":"M\u00e1rio Pedro","family":"Marques","sequence":"additional","affiliation":[{"name":"Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"given":"Catarina","family":"Melim","sequence":"additional","affiliation":[{"name":"Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9824-4753","authenticated-orcid":false,"given":"Carla","family":"Varela","sequence":"additional","affiliation":[{"name":"Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, University of Coimbra, P\u00f3lo II, R. Silvio Lima, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7014-4614","authenticated-orcid":false,"given":"Vilma A.","family":"Sard\u00e3o","sequence":"additional","affiliation":[{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Multidisciplinary Institute of Aging, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"given":"Jos\u00e9","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8744-7814","authenticated-orcid":false,"given":"Maria In\u00eas","family":"Dias","sequence":"additional","affiliation":[{"name":"Mountain Research Centre (CIMO), Polytechnic Institute of Bragan\u00e7a (IPB), Campus Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), Polytechnic Institute of Bragan\u00e7a (IPB), Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9050-5189","authenticated-orcid":false,"given":"Lillian","family":"Barros","sequence":"additional","affiliation":[{"name":"Mountain Research Centre (CIMO), Polytechnic Institute of Bragan\u00e7a (IPB), Campus Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), Polytechnic Institute of Bragan\u00e7a (IPB), Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"given":"Paulo J.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4562-6683","authenticated-orcid":false,"given":"C\u00e9lia","family":"Cabral","sequence":"additional","affiliation":[{"name":"Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,1]]},"reference":[{"key":"ref_1","unstructured":"(2024, October 07). 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