{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:30:19Z","timestamp":1766575819042,"version":"3.48.0"},"reference-count":120,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: This scoping review aimed to map evidence on metabolic alterations in the vaginal environment associated with dysbiosis, transient and persistent human papillomavirus (HPV) infection, and cervical dysplasia, highlighting potential metabolic and protein biomarkers for early detection of cervical cancer. Methods: Systematic searches were conducted in PubMed, Scopus, and Web of Science, following the JBI methodology and PRISMA-ScR guidelines. Studies jointly evaluating vaginal metabolites and proteins in women with HPV and cervical intraepithelial neoplasia (CIN) in the context of dysbiosis were included. Results: After duplicate removal, 196 records were screened, and 41 studies were selected\u2014mostly cross-sectional observational designs\u2014published between 2006 and 2025, predominantly by Chinese research groups. Lactobacillus spp. predominated in HPV-negative women, while HPV infection was associated with a dysbiotic environment enriched with anaerobes such as Gardnerella vaginalis, Atopobium vaginae, Prevotella, and Sneathia. Of 389 metabolic and protein markers associated with HPV infection and CIN, 44 underwent ROC analysis, with prolineaminopeptidase, 5\u2032-O-methylmelledonal, and calonectin showing high diagnostic performance (AUC &gt; 0.90). Conclusions: These results suggest vaginal microbiome and metabolic profiles may represent promising biomarkers for persistent HPV infection. Further, longitudinal studies with larger samples are needed for clinical validation.<\/jats:p>","DOI":"10.3390\/ph19010042","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:22:14Z","timestamp":1766575334000},"page":"42","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mapping the Vaginal Metabolic Profile in Dysbiosis, Persistent Human Papillomavirus Infection, and Cervical Intraepithelial Neoplasia: A Scoping Review"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4926-7684","authenticated-orcid":false,"given":"Edn\u00e9ia Peres","family":"Machado","sequence":"first","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1168-241X","authenticated-orcid":false,"given":"Allan Michael","family":"Junkert","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3434-1239","authenticated-orcid":false,"given":"Raul Edison Luna","family":"Lazo","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4728-4488","authenticated-orcid":false,"given":"Idonilton da Concei\u00e7\u00e3o","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-8608","authenticated-orcid":false,"given":"Fernanda Stumpf","family":"Tonin","sequence":"additional","affiliation":[{"name":"Pharmacy and Pharmaceutical Technology Department, Faculty of Pharmacy, University of Granada, 18012 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9951-587X","authenticated-orcid":false,"given":"Luana Mota","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"},{"name":"Department of Pharmacy, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9723-584X","authenticated-orcid":false,"given":"Helena Hiemisch Lobo","family":"Borba","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"},{"name":"Department of Pharmacy, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7049-4363","authenticated-orcid":false,"given":"Roberto","family":"Pontarolo","sequence":"additional","affiliation":[{"name":"Postgraduate Programme in Pharmaceutical Sciences, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"},{"name":"Department of Pharmacy, Universidade Federal do Paran\u00e1, Jardim Bot\u00e2nico, Curitiba 80210-170, PR, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"ref_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. 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