{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T19:01:06Z","timestamp":1772910066119,"version":"3.50.1"},"reference-count":106,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T00:00:00Z","timestamp":1544918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/FIS\/04650\/2013"],"award-info":[{"award-number":["UID\/FIS\/04650\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-032651"],"award-info":[{"award-number":["POCI-01-0145-FEDER-032651"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Many graphene-based materials (GBNs) applied to therapy and diagnostics (theranostics) in cancer have been developed. Most of them are hybrid combinations of graphene with other components (e.g., drugs or other bioactives, polymers, and nanoparticles) aiming toward a synergic theranostic effect. However, the role of graphene in each of these hybrids is sometimes not clear enough and the synergic graphene effect is not proven. The objective of this review is to elaborate on the role of GBNs in the studies evaluated and to compare the nanoformulations in terms of some of their characteristics, such as therapeutic outcomes and toxicity, which are essential features for their potential use as bionanosystems. A systematic review was carried out using the following databases: PubMed, Scopus, and ISI Web of Science (2013\u20132018). Additional studies were identified manually by consulting the references list of relevant reviews. Only English papers presenting at least one strategy for cancer therapy and one strategy for cancer diagnostics, and that clearly show the role of graphene in theranostics, were included. Data extraction and quality assessment was made by reviewer pairings. Fifty-five studies met the inclusion criteria, but they were too heterogeneous to combine in statistical meta-analysis. Critical analysis and discussion of the selected papers are presented.<\/jats:p>","DOI":"10.3390\/pharmaceutics10040282","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T02:15:59Z","timestamp":1545099359000},"page":"282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A Systematic Review and Critical Analysis of the Role of Graphene-Based Nanomaterials in Cancer Theranostics"],"prefix":"10.3390","volume":"10","author":[{"given":"Teresa","family":"Viseu","sequence":"first","affiliation":[{"name":"CF-UM-UP\u2014Centre of Physics of Universities of Minho and Porto, Departament of Physics of University of Minho, Escola de Ci\u00eancias, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5080-032X","authenticated-orcid":false,"given":"Carla M.","family":"Lopes","sequence":"additional","affiliation":[{"name":"FP-ENAS\/CEBIMED\u2014Fernando Pessoa Energy, Environment and Health Research Unit\/Biomedical Research Centre, Faculty of Health Sciences, Fernando Pessoa University, 4249-004 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-039X","authenticated-orcid":false,"given":"Eduarda","family":"Fernandes","sequence":"additional","affiliation":[{"name":"CF-UM-UP\u2014Centre of Physics of Universities of Minho and Porto, Departament of Physics of University of Minho, Escola de Ci\u00eancias, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Maria Elisabete C.D.","family":"Real Oliveira","sequence":"additional","affiliation":[{"name":"CF-UM-UP\u2014Centre of Physics of Universities of Minho and Porto, Departament of Physics of University of Minho, Escola de Ci\u00eancias, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2593-1672","authenticated-orcid":false,"given":"Marlene","family":"L\u00facio","sequence":"additional","affiliation":[{"name":"CF-UM-UP\u2014Centre of Physics of Universities of Minho and Porto, Departament of Physics of University of Minho, Escola de Ci\u00eancias, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,16]]},"reference":[{"key":"ref_1","unstructured":"WHO (2012). GLOBOCAN 2012: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012, International Agency for Research on Cancer."},{"key":"ref_2","unstructured":"WHO (2016). Cancer Prevention and Control in the Context of an Integrated Approach, WHO."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/nrd2614","article-title":"Nanoparticle therapeutics: An emerging treatment modality for cancer","volume":"7","author":"Davis","year":"2008","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nnano.2007.387","article-title":"Nanocarriers as an emerging platform for cancer therapy","volume":"2","author":"Peer","year":"2007","journal-title":"Nat. Nanotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.carbon.2018.07.058","article-title":"Graphene oxide\/MnWO4 nanocomposite for magnetic resonance\/photoacoustic dual-model imaging and tumor photothermo-chemotherapy","volume":"138","author":"Chang","year":"2018","journal-title":"Carbon"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1002\/smll.201502917","article-title":"A High-Sensitivity and Low-Power Theranostic Nanosystem for Cell SERS Imaging and Selectively Photothermal Therapy Using Anti-EGFR-Conjugated Reduced Graphene Oxide\/Mesoporous Silica\/AuNPs Nanosheets","volume":"12","author":"Chen","year":"2016","journal-title":"Small"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biomaterials.2016.04.006","article-title":"Nano-graphene oxide-mediated In vivo fluorescence imaging and bimodal photodynamic and photothermal destruction of tumors","volume":"95","author":"Kalluru","year":"2016","journal-title":"Biomaterials"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Su, Y.-L., Yu, T.-W., Chiang, W.-H., Chiu, H.-C., Chang, C.-H., Chiang, C.-S., and Hu, S.-H. (2017). Hierarchically Targeted and Penetrated Delivery of Drugs to Tumors by Size-Changeable Graphene Quantum Dot Nanoaircrafts for Photolytic Therapy. Adv. Funct. Mater., 27.","DOI":"10.1002\/adfm.201700056"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"135","DOI":"10.2174\/156800911794328493","article-title":"Advances in nano drugs for cancer chemotherapy","volume":"11","author":"Ali","year":"2011","journal-title":"Curr. Cancer Drug Targets"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/2052-8426-2-27","article-title":"Gene therapy for cancer: Present status and future perspective","volume":"2","author":"Amer","year":"2014","journal-title":"Mol. Cell. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"762","DOI":"10.7150\/thno.14988","article-title":"Current Approaches of Photothermal Therapy in Treating Cancer Metastasis with Nanotherapeutics","volume":"6","author":"Zou","year":"2016","journal-title":"Theranostics"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.redox.2017.11.018","article-title":"Developing the next generation of graphene-based platforms for cancer therapeutics: The potential role of reactive oxygen species","volume":"15","author":"Tabish","year":"2018","journal-title":"Redox Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.2174\/0929867323666161019141817","article-title":"Recent developments of phototherapy based on graphene family nanomaterials","volume":"24","author":"Zhang","year":"2017","journal-title":"Curr. Med. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Spirou, S.V., Basini, M., Lascialfari, A., Sangregorio, C., and Innocenti, C. (2018). Magnetic Hyperthermia and Radiation Therapy: Radiobiological Principles and Current Practice (\u2020). Nanomaterials, 8.","DOI":"10.3390\/nano8060401"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1166\/jbn.2016.2258","article-title":"Nanoparticulate contrast agents for multimodality molecular imaging","volume":"12","author":"Xia","year":"2016","journal-title":"J. Biomed. Nanotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1021\/acs.chemrev.6b00073","article-title":"Nanomaterials for In Vivo Imaging","volume":"117","author":"Smith","year":"2017","journal-title":"Chem. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1002\/cmmi.1719","article-title":"Dual nano-sized contrast agents in PET\/MRI: A systematic review","volume":"11","author":"Lahooti","year":"2016","journal-title":"Contrast Med. Mol. Imaging"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2414","DOI":"10.3390\/s120302414","article-title":"Upconversion nanomaterials: Synthesis, mechanism, and applications in sensing","volume":"12","author":"Chen","year":"2012","journal-title":"Sensors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1021\/bc5003967","article-title":"Upconversion Nanoparticles: A Versatile Solution to Multiscale Biological Imaging","volume":"26","author":"Wu","year":"2015","journal-title":"Bioconjug. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"T154","DOI":"10.1016\/j.neuroimage.2009.03.043","article-title":"Infrared thermal imaging: A review of the literature and case report","volume":"47","author":"Kateb","year":"2009","journal-title":"NeuroImage"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.apenergy.2014.08.005","article-title":"Infrared thermography (IRT) applications for building diagnostics: A review","volume":"134","author":"Kylili","year":"2014","journal-title":"Appl. Energy"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1155\/2018\/8619342","article-title":"Raman Spectroscopy and Imaging for Cancer Diagnosis","volume":"2018","author":"Cui","year":"2018","journal-title":"J. Healthc. Eng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.addr.2015.03.009","article-title":"Raman spectroscopy for medical diagnostics\u2014From in-vitro biofluid assays to in-vivo cancer detection","volume":"89","author":"Kong","year":"2015","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Yu, J., Kahkoska, A.R., and Gu, Z. (2017). Photoacoustic Drug Delivery. Sensors, 17.","DOI":"10.3390\/s17061400"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1126\/science.1102896","article-title":"Electric Field Effect in Atomically Thin Carbon Films","volume":"306","author":"Novoselov","year":"2004","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1103\/RevModPhys.81.109","article-title":"The electronic properties of graphene","volume":"81","author":"Guinea","year":"2009","journal-title":"Rev. Mod. Phys."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1080\/10408430903505036","article-title":"Synthesis of Graphene and Its Applications: A Review","volume":"35","author":"Choi","year":"2010","journal-title":"Crit. Rev. Solid State Mater. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10876","DOI":"10.1021\/ja803688x","article-title":"PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs","volume":"130","author":"Liu","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s40820-018-0206-4","article-title":"A Review on Graphene-Based Nanomaterials in Biomedical Applications and Risks in Environment and Health","volume":"10","author":"McShan","year":"2018","journal-title":"Nano-Micro Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1016\/j.drudis.2017.04.002","article-title":"Graphene-based nanomaterials for drug and\/or gene delivery, bioimaging, and tissue engineering","volume":"22","author":"Zhao","year":"2017","journal-title":"Drug Discov. Today"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Demchenko, A.P., and Dekaliuk, M.O. (2013). Novel fluorescent carbonic nanomaterials for sensing and imaging. Methods Appl. Fluoresc., 1.","DOI":"10.1088\/2050-6120\/1\/4\/042001"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jconrel.2013.10.017","article-title":"Graphene-based nanomaterials for drug delivery and tissue engineering","volume":"173","author":"Goenka","year":"2014","journal-title":"J. Control. Release"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1039\/C2CS35342C","article-title":"Nano-graphene in biomedicine: Theranostic applications","volume":"42","author":"Yang","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1002\/wnan.1342","article-title":"Graphene-based nanomaterials as molecular imaging agents","volume":"7","author":"Garg","year":"2015","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s11671-010-9751-6","article-title":"Biocompatibility of Graphene Oxide","volume":"6","author":"Wang","year":"2010","journal-title":"Nanoscale Res. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1016\/j.carbon.2010.11.005","article-title":"Distribution and biocompatibility studies of graphene oxide in mice after intravenous administration","volume":"49","author":"Zhang","year":"2011","journal-title":"Carbon"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.toxlet.2010.11.016","article-title":"In vitro toxicity evaluation of graphene oxide on A549 cells","volume":"200","author":"Chang","year":"2011","journal-title":"Toxicol. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"76556","DOI":"10.1039\/C5RA13831K","article-title":"The non-innocent nature of graphene oxide as a theranostic platform for biomedical applications and its reactivity towards metal-based anticancer drugs","volume":"5","author":"Mokdad","year":"2015","journal-title":"RSC Adv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s12989-016-0168-y","article-title":"Toxicity of graphene-family nanoparticles: A general review of the origins and mechanisms","volume":"13","author":"Ou","year":"2016","journal-title":"Part. Fibre Toxicol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Moher, D., Liberati, A., Tetzlaff, J., Altman, D.G., and The, P.G. (2009). Preferred Reporting Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement. PLoS Med., 6.","DOI":"10.1371\/journal.pmed.1000097"},{"key":"ref_41","unstructured":"Kmet, L., Cook, L., and Lee, R. (2018, October 17). Standard Quality Assessment Criteria for Evaluating Primary Research Papers from a Variety of Fields. Available online: https:\/\/www.ihe.ca\/advanced-search\/standard-quality-assessment-criteria-for-evaluating-primary-research-papers-from-a-variety-of-fields."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.drudis.2017.09.013","article-title":"Carbon nanomaterials in oncology: An expanding horizon","volume":"23","author":"Mehra","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.pmatsci.2017.08.004","article-title":"Graphene: A versatile platform for nanotheranostics and tissue engineering","volume":"91","author":"Ninan","year":"2018","journal-title":"Prog. Mater. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1038\/ncomms5596","article-title":"A graphene quantum dot photodynamic therapy agent with high singlet oxygen generation","volume":"5","author":"Ge","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"12413","DOI":"10.1021\/am504071z","article-title":"Photoluminescent Graphene Nanoparticles for Cancer Phototherapy and Imaging","volume":"6","author":"Nurunnabi","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1002\/smll.201203106","article-title":"Graphene Nanomesh Promises Extremely Efficient In Vivo Photothermal Therapy","volume":"9","author":"Akhavan","year":"2013","journal-title":"Small"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jconrel.2016.05.007","article-title":"Graphene oxide-incorporated pH-responsive folate-albumin-photosensitizer nanocomplex as image-guided dual therapeutics","volume":"234","author":"Battogtokh","year":"2016","journal-title":"J. Control. Release"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"9988","DOI":"10.1021\/acs.inorgchem.8b01159","article-title":"Quad-Model Imaging-Guided High-Efficiency Phototherapy Based on Upconversion Nanoparticles and ZnFe2O4 Integrated Graphene Oxide","volume":"57","author":"Bi","year":"2018","journal-title":"Inorg. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1021\/acsami.6b13150","article-title":"Aptamer-Conjugated Graphene Quantum Dots\/Porphyrin Derivative Theranostic Agent for Intracellular Cancer-Related MicroRNA Detection and Fluorescence-Guided Photothermal\/Photodynamic Synergetic Therapy","volume":"9","author":"Cao","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"84980","DOI":"10.1039\/C5RA17143A","article-title":"Fe2O3@Au core@shell nanoparticle-graphene nanocomposites as theranostic agents for bioimaging and chemo-photothermal synergistic therapy","volume":"5","author":"Chen","year":"2015","journal-title":"RSC Adv."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.biomaterials.2015.06.043","article-title":"Radionuclide I-131 labeled reduced graphene oxide for nuclear imaging guided combined radio- and photothermal therapy of cancer","volume":"66","author":"Chen","year":"2015","journal-title":"Biomaterials"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1039\/c2cc36297j","article-title":"A graphene oxide-photosensitizer complex as an enzyme-activatable theranostic agent","volume":"49","author":"Cho","year":"2013","journal-title":"Chem. Commun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/cplu.201500349","article-title":"Grafting of ZnS:Mn-doped nanocrystals and an anticancer drug onto graphene oxide for delivery and cell labeling","volume":"81","author":"Dinda","year":"2016","journal-title":"ChemPlusChem"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"27396","DOI":"10.1021\/acsami.7b08824","article-title":"Beyond a Carrier: Graphene Quantum Dots as a Probe for Programmatically Monitoring Anti-Cancer Drug Delivery, Release, and Response","volume":"9","author":"Ding","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"11015","DOI":"10.1021\/acsami.5b02803","article-title":"Multifunctional Poly(L-lactide)-Polyethylene Glycol-Grafted Graphene Quantum Dots for Intracellular MicroRNA Imaging and Combined Specific-Gene-Targeting Agents Delivery for Improved Therapeutics","volume":"7","author":"Dong","year":"2015","journal-title":"Acs Appl. Mater. Interfaces"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.biomaterials.2015.11.041","article-title":"Hybrid graphene\/Au activatable theranostic agent for multimodalities imaging guided enhanced photothermal therapy","volume":"79","author":"Gao","year":"2016","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3931","DOI":"10.1039\/C7DT04141A","article-title":"Nano-graphene oxide-UCNP-Ce6 covalently constructed nanocomposites for NIR-mediated bioimaging and PTT\/PDT combinatorial therapy","volume":"47","author":"Gulzar","year":"2018","journal-title":"Dalton Trans."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5783","DOI":"10.1039\/C7TB01600J","article-title":"Facile fabrication of a resveratrol loaded phospholipid@reduced graphene oxide nanoassembly for targeted and near-infrared laser-triggered chemo\/photothermal synergistic therapy of cancer in vivo","volume":"5","author":"Hai","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.7150\/thno.14566","article-title":"Indocyanine Green-Loaded Polydopamine-Reduced Graphene Oxide Nanocomposites with Amplifying Photoacoustic and Photothermal Effects for Cancer Theranostics","volume":"6","author":"Hu","year":"2016","journal-title":"Theranostics"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1039\/C4NR06616B","article-title":"Facile integration of multiple magnetite nanoparticles for theranostics combining efficient MRI and thermal therapy","volume":"7","author":"Huang","year":"2015","journal-title":"Nanoscale"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1016\/j.biomaterials.2013.03.027","article-title":"Graphene oxide modified PLA microcapsules containing gold nanoparticles for ultrasonic\/CT bimodal imaging guided photothermal tumor therapy","volume":"34","author":"Jin","year":"2013","journal-title":"Biomaterials"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"10680","DOI":"10.1039\/C5NR01075F","article-title":"A hyaluronic acid nanogel for photo-chemo theranostics of lung cancer with simultaneous light-responsive controlled release of doxorubicin","volume":"7","author":"Khatun","year":"2015","journal-title":"Nanoscale"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"11420","DOI":"10.1039\/C6RA25949A","article-title":"Graphene quantum dot-based theranostic agents for active targeting of breast cancer","volume":"7","author":"Ko","year":"2017","journal-title":"RSC Adv."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1039\/C7BM01113J","article-title":"Lanthanide-doped upconversion nanoparticles complexed with nano-oxide graphene used for upconversion fluorescence imaging and photothermal therapy","volume":"6","author":"Li","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"22332","DOI":"10.1021\/acsami.7b07267","article-title":"Exceptionally High Payload of the IR780 Iodide on Folic Acid-Functionalized Graphene Quantum Dots for Targeted Photothermal Therapy","volume":"9","author":"Li","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1039\/C5NR07552A","article-title":"Dual-enhanced photothermal conversion properties of reduced graphene oxide-coated gold superparticles for light-triggered acoustic and thermal theranostics","volume":"8","author":"Lin","year":"2016","journal-title":"Nanoscale"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"17176","DOI":"10.1021\/acsami.6b05383","article-title":"Multifunctional Photosensitizer Grafted on Polyethylene Glycol and Polyethylenimine Dual-Functionalized Nanographene Oxide for Cancer-Targeted Near-Infrared Imaging and Synergistic Phototherapy","volume":"8","author":"Luo","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"6495","DOI":"10.1039\/c3tb21385d","article-title":"Graphene oxide wrapped gold nanoparticles for intracellular Raman imaging and drug delivery","volume":"1","author":"Ma","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jconrel.2015.05.280","article-title":"Image-guided synergistic photothermal therapy using photoresponsive imaging agent-loaded graphene-based nanosheets","volume":"211","author":"Miao","year":"2015","journal-title":"J. Control. Release"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"16395","DOI":"10.1021\/am504795d","article-title":"Multifunctional Hybrid Nanopatches of Graphene Oxide and Gold Nanostars for Ultraefficient Photothermal Cancer Therapy","volume":"6","author":"Nergiz","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"12141","DOI":"10.1021\/nn505989e","article-title":"Early-stage imaging of nanocarrier-enhanced chemotherapy response in living subjects by scalable photoacoustic microscopy","volume":"8","author":"Nie","year":"2014","journal-title":"ACS Nano"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.1002\/smll.201403395","article-title":"Fluorescence quenching nanoprobes dedicated to in vivo photoacoustic imaging and high-efficient tumor therapy in deep-seated tissue","volume":"11","author":"Qin","year":"2015","journal-title":"Small"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1039\/C5RA24752G","article-title":"Fluorescence dye loaded nano-graphene for multimodal imaging guided photothermal therapy","volume":"6","author":"Rong","year":"2016","journal-title":"RSC Adv."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5236","DOI":"10.1016\/j.biomaterials.2013.03.090","article-title":"Protein-assisted fabrication of nano-reduced graphene oxide for combined in vivo photoacoustic imaging and photothermal therapy","volume":"34","author":"Sheng","year":"2013","journal-title":"Biomaterials"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1007\/s11095-016-1891-7","article-title":"A Multi-Functional Tumor Theranostic Nanoplatform for MRI Guided Photothermal-Chemotherapy","volume":"33","author":"Shi","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5847","DOI":"10.1016\/j.biomaterials.2014.03.042","article-title":"A tumor-targeting near-infrared laser-triggered drug delivery system based on GO@Ag nanoparticles for chemo-photothermal therapy and X-ray imaging","volume":"35","author":"Shi","year":"2014","journal-title":"Biomaterials"},{"key":"ref_77","first-page":"762","article-title":"Bacteriomimetic poly-\u03b3-glutamic acid surface coating for hemocompatibility and safety of nanomaterials","volume":"11","author":"Shim","year":"2017","journal-title":"Nanotoxicology"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"6314","DOI":"10.1038\/srep06314","article-title":"Cancer Therapy Using Ultrahigh Hydrophobic Drug-Loaded Graphene Derivatives","volume":"4","author":"Some","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"9199","DOI":"10.1021\/acsnano.5b03804","article-title":"Sequential Drug Release and Enhanced Photothermal and Photoacoustic Effect of Hybrid Reduced Graphene Oxide-Loaded Ultrasmall Gold Nanorod Vesicles for Cancer Therapy","volume":"9","author":"Song","year":"2015","journal-title":"ACS Nano"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.bios.2016.10.076","article-title":"A graphene quantum dot@Fe3O4@SiO2 based nanoprobe for drug delivery sensing and dual-modal fluorescence and MRI imaging in cancer cells","volume":"92","author":"Su","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.2147\/IJN.S81097","article-title":"Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy","volume":"10","author":"Taratula","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.msec.2016.05.007","article-title":"Milk-derived multi-fluorescent graphene quantum dot-based cancer theranostic system","volume":"67","author":"Thakur","year":"2016","journal-title":"Mater. Sci. Eng. C Mater. Boil. Appl."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1039\/C4FD00074A","article-title":"Aptamer-conjugated theranostic hybrid graphene oxide with highly selective biosensing and combined therapy capability","volume":"175","author":"Pramanik","year":"2014","journal-title":"Faraday Discuss."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1039\/C5TB00211G","article-title":"A two-component active targeting theranostic agent based on graphene quantum dots","volume":"3","author":"Wang","year":"2015","journal-title":"J. Mater. Chem. B"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"7715","DOI":"10.1016\/j.biomaterials.2013.06.045","article-title":"Graphene oxide covalently grafted upconversion nanoparticles for combined NIR mediated imaging and photothermal\/photodynamic cancer therapy","volume":"34","author":"Wang","year":"2013","journal-title":"Biomaterials"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"5762","DOI":"10.1039\/c3tb20986e","article-title":"Dye-enhanced graphene oxide for photothermal therapy and photoacoustic imaging","volume":"1","author":"Wang","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.actbio.2017.01.078","article-title":"Single wavelength light-mediated, synergistic bimodal cancer photoablation and amplified photothermal performance by graphene\/gold nanostar\/photosensitizer theranostics","volume":"53","author":"Wu","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.1039\/C4NR06868H","article-title":"Optical and photoacoustic dual-modality imaging guided synergistic photodynamic\/photothermal therapies","volume":"7","author":"Yan","year":"2015","journal-title":"Nanoscale"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1021\/acs.langmuir.6b04189","article-title":"Mesoporous Silica Nanoparticles Capped with Graphene Quantum Dots for Potential Chemo\u2013Photothermal Synergistic Cancer Therapy","volume":"33","author":"Yao","year":"2017","journal-title":"Langmuir"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1600804","DOI":"10.1002\/adhm.201600804","article-title":"Improved Anticancer Photothermal Therapy Using the Bystander Effect Enhanced by Antiarrhythmic Peptide Conjugated Dopamine-Modified Reduced Graphene Oxide Nanocomposite","volume":"6","author":"Yu","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"15460","DOI":"10.1039\/C5RA25518J","article-title":"Co-delivery of paclitaxel and indocyanine green by PEGylated graphene oxide: A potential integrated nanoplatform for tumor theranostics","volume":"6","author":"Zhang","year":"2016","journal-title":"RSC Adv."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1039\/C6TB02137A","article-title":"Hydrophilic graphene oxide\/bismuth selenide nanocomposites for CT imaging, photoacoustic imaging, and photothermal therapy","volume":"5","author":"Zhang","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"11167","DOI":"10.1039\/C5RA24785C","article-title":"Multifunctional human serum albumin-modified reduced graphene oxide for targeted photothermal therapy of hepatocellular carcinoma","volume":"6","author":"Zheng","year":"2016","journal-title":"RSC Adv."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1039\/C4CC06968D","article-title":"Multicolor imaging and the anticancer effect of a bifunctional silica nanosystem based on the complex of graphene quantum dots and hypocrellin A","volume":"51","author":"Zhou","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1039\/C7BM00008A","article-title":"Recent advances in carbon based nanosystems for cancer theranostics","volume":"5","author":"Augustine","year":"2017","journal-title":"Biomater. Sci."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.pneurobio.2018.04.004","article-title":"Lipid nanocarriers loaded with natural compounds: Potential new therapies for age related neurodegenerative diseases?","volume":"168","author":"Soares","year":"2018","journal-title":"Prog. Neurobiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"710","DOI":"10.7150\/thno.11387","article-title":"Graphene as cancer theranostic tool: Progress and future challenges","volume":"5","author":"Orecchioni","year":"2015","journal-title":"Theranostics"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1039\/C3TB21281E","article-title":"Imaging guided photothermal therapy using iron oxide loaded poly(lactic acid) microcapsules coated with graphene oxide","volume":"2","author":"Li","year":"2014","journal-title":"J. Mater. Chem. B"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1002\/adma.201502843","article-title":"Near-Infrared-Sensitive Materials Based on Upconverting Nanoparticles","volume":"28","author":"Wu","year":"2016","journal-title":"Adv. Mater."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1002\/smll.201402648","article-title":"Glowing graphene quantum dots and carbon dots: Properties, syntheses, and biological applications","volume":"11","author":"Zheng","year":"2015","journal-title":"Small"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"283","DOI":"10.7150\/thno.3642","article-title":"Biomedical applications of graphene","volume":"2","author":"Shen","year":"2012","journal-title":"Theranostics"},{"key":"ref_102","unstructured":"Grumezescu, A.M. (2018). Chapter 8\u2014Graphene-based materials for application in pharmaceutical nanotechnology. Fullerens, Graphenes and Nanotubes, William Andrew Publishing."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4400","DOI":"10.1039\/C7CS00363C","article-title":"Promises, facts and challenges for graphene in biomedical applications","volume":"46","author":"Reina","year":"2017","journal-title":"Chem. Soc. Rev."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"4986","DOI":"10.1002\/anie.201209099","article-title":"Graphene: Safe or Toxic? The Two Faces of the Medal","volume":"52","author":"Bianco","year":"2013","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_105","unstructured":"SCENIHR (2014). Position Statement on Emerging and Newly Identified Health Risks to be Drawn to the Attention of the European Commission, EU Commission."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1039\/C0NR00647E","article-title":"Low-toxic and safe nanomaterials by surface-chemical design, carbon nanotubes, fullerenes, metallofullerenes, and graphenes","volume":"3","author":"Yan","year":"2011","journal-title":"Nanoscale"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/10\/4\/282\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:34:22Z","timestamp":1760196862000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/10\/4\/282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,16]]},"references-count":106,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["pharmaceutics10040282"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics10040282","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,16]]}}}