{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T21:05:49Z","timestamp":1776287149243,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T00:00:00Z","timestamp":1594425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/11350-6"],"award-info":[{"award-number":["2018\/11350-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/13432-0"],"award-info":[{"award-number":["2018\/13432-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2011\/21219-5"],"award-info":[{"award-number":["2011\/21219-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["425271\/2016-1"],"award-info":[{"award-number":["425271\/2016-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The formation of mucosal ulcers is an end result of epithelial damage, and it occurs due to some specific causes, such as trauma, aphthous stomatitis, lichen planus and lichenoid reactions, cytotoxic effects of chemotherapy and radiation, and drug-induced hypersensitivity reactions and malignant settings. This study focused on films for target drug delivery with respect to the treatment of the diseases of the oral mucosa, specifically mucositis. The results of a single clinical study as a pre-experimental design was performed and followed up to the outcome until 30 days. The polymeric film was prepared in a mucoadhesive bilayer structure: the basal layer with lidocaine HCl had a faster release than the apical layer with benzydamine HCl and N-acetyl-cysteine. Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and SEM characterized the physical\u2013chemical and morphological properties. The cell viability and cytotoxicity were evaluated in cell line MCF7. The transport mechanism of the solvent (swelling) and the drugs in the basal or apical layer (drug release) was explained with mathematical models. To evaluate the effect of movement inside the mouth, the folding endurance was determined. The mucoadhesive bilayer film is biologically safe and stimulates cellular proliferation. A single study in vivo demonstrated the therapeutic effect of the mucoadhesive bilayer film in buccal mucositis.<\/jats:p>","DOI":"10.3390\/pharmaceutics12070657","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T11:03:23Z","timestamp":1594724603000},"page":"657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Bilayer Mucoadhesive Buccal Film for Mucosal Ulcers Treatment: Development, Characterization, and Single Study Case"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3586-9457","authenticated-orcid":false,"given":"Thais F. R.","family":"Alves","sequence":"first","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"}]},{"given":"Alesssandra C.","family":"Rios","sequence":"additional","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-9035","authenticated-orcid":false,"given":"Katiusca","family":"da Silva Pontes","sequence":"additional","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"}]},{"given":"Decio L.","family":"Portella","sequence":"additional","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"},{"name":"Department of Surgery Plastic, School of Medicine, Pontifical Catholic University, Sorocaba 18030-070, Sao Paulo, Brazil"}]},{"given":"Norberto","family":"Aranha","sequence":"additional","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6527-6612","authenticated-orcid":false,"given":"Patricia","family":"Severino","sequence":"additional","affiliation":[{"name":"Biotechnological Postgraduate Program, University of Tiradentes (Unit), Aracaju 49010-390, Sergipe, Brazil"},{"name":"Nanomedicine and Nanotechnology Laboratory (LNMed), Institute of Technology and Research (ITP), Aracaju 49010-390, Sergipe, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9737-6017","authenticated-orcid":false,"given":"Eliana B.","family":"Souto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Centre of Biological Engineering (CEB), University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Joyce K. M.","family":"Gonsalves","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Sergipe, Saint Christopher, Sergipe 49100-000, Brazil"}]},{"given":"Rogeria","family":"de Souza Nunes","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Sergipe, Saint Christopher, Sergipe 49100-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3618-8415","authenticated-orcid":false,"given":"Marco V.","family":"Chaud","sequence":"additional","affiliation":[{"name":"Laboratory of Biomaterials and Nanotechnology (LaBNUS), University of Sorocaba, Sorocaba 18023-000, Sao Paulo, Brazil"},{"name":"College of Engineering of Bioprocess and Biotechnology, University of Sorocaba, Sorocaba1 8023-000, Sao Paulo, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,11]]},"reference":[{"key":"ref_1","first-page":"563","article-title":"Treatment of Radiation-Induced Oral Mucositis Using a Novel Accepted Taste of Prolonged Release Mucoadhesive Bi-medicated Double-Layer Buccal Films","volume":"18","author":"Elmonem","year":"2016","journal-title":"AAPS PharmSciTech"},{"key":"ref_2","first-page":"e48","article-title":"Comparison of triamcinolone acetonide mucoadhesive film with licorice mucoadhesive film on radiotherapy-induced oral mucositis: A randomized double-blinded clinical trial, Asia","volume":"13","author":"Ghalayani","year":"2017","journal-title":"Pac. 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