{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T22:27:53Z","timestamp":1776896873895,"version":"3.51.2"},"reference-count":49,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Argentinean Agency for the Scientific and Technological Promotion (ANPCyT)","award":["Projects PICT (2017)\/1344; PICT start-up (2016)\/4808"],"award-info":[{"award-number":["Projects PICT (2017)\/1344; PICT start-up (2016)\/4808"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Polymer\u2013liposome complexes (PLCs) can be efficiently applied for the treatment and\/or diagnosis of several types of diseases, such as cancerous, dermatological, neurological, ophthalmic and orthopedic. In this work, temperature-\/pH-sensitive PLC-based systems for controlled release were developed and characterized. The selected hydrophilic polymeric setup consists of copolymers of Pluronic\u00ae-poly(acrylic acid) (PLU-PAA) and Pluronic\u00ae-poly(N,N-dimethylaminoethyl methacrylate) (PLU-PD) synthesized by atom transfer radical polymerization (ATRP). The copolymers were incorporated into liposomes formulated from soybean lecithin, with different copolymer\/phospholipid ratios (2.5, 5 and 10%). PLCs were characterized by evaluating their particle size, polydispersity, surface charge, capacity of release and encapsulation efficiency. Their cytotoxic potential was assessed by determining the viability of human epithelial cells exposed to them. The results showed that the incorporation of the synthesized copolymers positively contributed to the stabilization of the liposomes. The main accomplishments of this work were the innovative synthesis of PLU-PD and PLU-PAA by ATRP, and the liposome stabilization by their incorporation. The formulated PLCs exhibited relevant characteristics, notably stimuli-responsive attributes upon slight changes in pH and\/or temperature, with proven absence of cellular toxicity, which could be of interest for the treatment or diagnosis of all diseases that cause some particular pH\/temperature change in the target area.<\/jats:p>","DOI":"10.3390\/pharmaceutics14040735","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:41:30Z","timestamp":1648590090000},"page":"735","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Formulation and Characterization of Stimuli-Responsive Lecithin-Based Liposome Complexes with Poly(acrylic acid)\/Poly(N,N-dimethylaminoethyl methacrylate) and Pluronic\u00ae Copolymers for Controlled Drug Delivery"],"prefix":"10.3390","volume":"14","author":[{"given":"M\u00f3nica G.","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"CIEPQPF, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima, P\u00f3lo II-Pinhal de Marrocos, 3030-790 Coimbra, Portugal"}]},{"given":"Ayelen","family":"Hugo","sequence":"additional","affiliation":[{"name":"Center for Research and Development in Food Cryotechnology (CIDCA, CCT-CONICET), La Plata 1900, Argentina"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8705-0160","authenticated-orcid":false,"given":"Andrea","family":"G\u00f3mez-Zavaglia","sequence":"additional","affiliation":[{"name":"Center for Research and Development in Food Cryotechnology (CIDCA, CCT-CONICET), La Plata 1900, Argentina"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5068-950X","authenticated-orcid":false,"given":"Pedro N.","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"CIEPQPF, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima, P\u00f3lo II-Pinhal de Marrocos, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8943-8329","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Alves","sequence":"additional","affiliation":[{"name":"CIEPQPF, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima, P\u00f3lo II-Pinhal de Marrocos, 3030-790 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10938","DOI":"10.1021\/acs.chemrev.5b00046","article-title":"New Developments in Liposomal Drug Delivery","volume":"115","author":"Pattni","year":"2015","journal-title":"Chem. 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