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Marto","doi-asserted-by":"publisher","award":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"],"award-info":[{"award-number":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"],"award-info":[{"award-number":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"Associate Laboratory for Green Chemistry (LAQV)","doi-asserted-by":"publisher","award":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"],"award-info":[{"award-number":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\/MCTES","doi-asserted-by":"publisher","award":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"],"award-info":[{"award-number":["UID\/04462","UID 04138","CEECINST\/00145\/2018","LA\/P\/0087\/2020","ERC-2016-CoG 725034","LA\/P\/0008\/2020","UIDP\/50006\/2020","UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Recent studies highlight the excellent wound-healing properties of collagen and chitosan materials. Combining these polymers with a bioactive compound could enhance their effectiveness as next-generation wound dressings. Hydroxytyrosol (HT), an antioxidant derived from olive oil, may aid wound healing due to its anti-inflammatory, antimicrobial, and angiogenesis-stimulating properties, making it a beneficial addition to collagen\u2013chitosan dressings. It could be a beneficial addition to collagen\u2013chitosan dressings, thus improving their therapeutic effects. This study screens the potential of collagen\u2013chitosan composites with HT for wound-healing applications and assesses the influence of the compound\u2019s incorporation on the materials\u2019 properties. Methods: The material production involved incorporating chitosan and HT into a marine collagen extract. The resulting collagen\u2013chitosan\u2013HT material was obtained through freeze-drying. Prototype dressing characterization included morphology by scanning electron microscopy, solid and hydrated state by textural and rheological studies, and in vitro HT release studies. The materials\u2019 cytocompatibility screening was assessed using a mouse fibroblast cell line, and the antibacterial activity was evaluated against microorganisms commonly implicated in wound infections. Results: Burst strength, viscosity, frequency sweep test, tackiness, and adhesion results indicate that chitosan contributes to the material\u2019s mechanical robustness by maintaining a high viscosity and preserving the material\u2019s gel structure. The in vitro release studies suggest an HT-controlled release profile with a maximum release (70%) achieved after 10 h. Biological experiments confirmed the materials\u2019 cytocompatibility with skin cells and very promising antibacterial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa. Conclusions: In conclusion, HT was successfully incorporated into a collagen\u2013chitosan matrix, enhancing the therapeutic prospect of the resultant material. The collagen\u2013chitosan\u2013HT composite presents a promising potential as an advanced wound-healing material.<\/jats:p>","DOI":"10.3390\/pharmaceutics17050618","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T05:54:43Z","timestamp":1746510883000},"page":"618","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Collagen\u2013Chitosan Composites Enhanced with Hydroxytyrosol for Prospective Wound Healing Uses"],"prefix":"10.3390","volume":"17","author":[{"given":"Miguel P.","family":"Batista","sequence":"first","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"LAQV-REQUIMTE, Associated Laboratory for Green Chemistry\u2014Network of Chemical and Technology, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7620-5194","authenticated-orcid":false,"given":"Margarida","family":"Pimenta","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3902-2966","authenticated-orcid":false,"given":"Naiara","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"}]},{"given":"Ana Rita C.","family":"Duarte","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Associated Laboratory for Green Chemistry\u2014Network of Chemical and Technology, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal"}]},{"given":"Maria do Ros\u00e1rio","family":"Bronze","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"FFULisboa, Faculty of Pharmacy, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5523-5622","authenticated-orcid":false,"given":"Joana","family":"Marto","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-6230","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric Bustos","family":"Gaspar","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1089\/wound.2015.0635","article-title":"Challenges in the Treatment of Chronic Wounds","volume":"4","author":"Frykberg","year":"2015","journal-title":"Adv. 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