{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:33:37Z","timestamp":1760060017308,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq","award":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"],"award-info":[{"award-number":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"]}]},{"name":"FUNCAP UNIVERSAL","award":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"],"award-info":[{"award-number":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"]}]},{"name":"FUNCAP-INTERNACIONALIZA\u00c7\u00c3O","award":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"],"award-info":[{"award-number":["306008\/2022-0","UNI-0210-00337.01.00\/23","ITR-0214-00060.01.00\/23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: The incorporation of bioactive molecules into mesoporous carriers is a promising strategy to improve stability, solubility, and therapeutic efficacy. In this study, we report for the first time the encapsulation of the synthetic chalcone 4-Cl into KIT-6 mesoporous silica and evaluate its cytotoxicity, toxicological profile, and pharmacological activities (antinociceptive, anti-inflammatory, and anxiolytic) using an in vivo zebrafish (Danio rerio) model. Methods: Zebrafish were orally dosed with 4-Cl, 4-Cl\/KIT-6, or KIT-6 (4, 20, 40 mg\/kg) and mortality was recorded for 96 h. For analgesia, zebrafish pretreated with 4-Cl, 4-Cl\/KIT-6, KIT-6, or morphine received a tail stimulus (0.1% formalin). Locomotor activity (quadrant crossings) was monitored for 30 min to assess analgesia (neurogenic: 0\u20135 min; inflammatory: 15\u201330 min). For inflammation, abdominal edema and weight gain were assessed 4 h after intraperitoneal carrageenan (1.5%). Zebrafish (n = 6\/group) received 4-Cl, 4-Cl\/KIT-6, or KIT-6 (4, 20, 40 mg\/kg, p.o.). Controls received ibuprofen (100 mg\/kg, p.o.) or 3% DMSO. Weight was measured hourly for 4 h post-carrageenan (difference between baseline and hourly weights). Results: Physicochemical characterizations confirmed successful encapsulation without compromising the ordered structure of KIT-6, as evidenced by a significant reduction in surface area and pore volume, indicating efficient drug incorporation. In vivo assays demonstrated that the 4-Cl\/KIT-6 formulation maintained the pharmacological activities of the free chalcone, reduced toxicity, and, notably, revealed a significant anxiolytic effect for the first time. Conclusions: These findings highlight KIT-6 as a promising platform for chalcone delivery systems and provide a solid basis for future preclinical investigations.<\/jats:p>","DOI":"10.3390\/pharmaceutics17080981","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T07:37:23Z","timestamp":1753861043000},"page":"981","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioactive Chalcone-Loaded Mesoporous Silica KIT-6 Nanocarrier: A Promising Strategy for Inflammation and Pain Management in Zebrafish"],"prefix":"10.3390","volume":"17","author":[{"given":"Maria Kueirislene Am\u00e2ncio","family":"Ferreira","sequence":"first","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"}]},{"given":"Francisco Rogenio Silva","family":"Mendes","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4774-8775","authenticated-orcid":false,"given":"Emmanuel Silva","family":"Marinho","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3598-8528","authenticated-orcid":false,"given":"Roberto","family":"Lima de Albuquerque","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3376-0789","authenticated-orcid":false,"given":"Jesyka Macedo","family":"Guedes","sequence":"additional","affiliation":[{"name":"Center for Exact Sciences and Technology, Vale do Acara\u00fa University, Sobral 62040-370, Brazil"}]},{"given":"Izabell Maria Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Federal University of Cear\u00e1, Fortaleza 60430-160, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3109-9683","authenticated-orcid":false,"given":"Ramon R\u00f3seo Paula Pessoa Bezerra de","family":"Menezes","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Federal University of Cear\u00e1, Fortaleza 60430-160, Brazil"}]},{"given":"Vinicius Patricio Santos","family":"Caldeira","sequence":"additional","affiliation":[{"name":"Laboratory of Catalysis, Environment and Materials, Department of Chemistry, Rio Grande do Norte State University, Mossor\u00f3 59610-210, Brazil"}]},{"given":"Anne Gabriella Dias","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Catalysis, Environment and Materials, Department of Chemistry, Rio Grande do Norte State University, Mossor\u00f3 59610-210, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8254-4727","authenticated-orcid":false,"given":"Marisa J\u00e1dna Silva","family":"Frederico","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Physiology, Center for Drug Research and Development, School of Medicine, Federal University of Cear\u00e1, Fortaleza 60430-275, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5757-334X","authenticated-orcid":false,"given":"Ant\u00f4nio C\u00e9sar Honorato","family":"Barreto","sequence":"additional","affiliation":[{"name":"Department of Physics, Federal University of Ceara, Fortaleza 60020-181, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1123-9167","authenticated-orcid":false,"given":"In\u00eas","family":"Domingues","sequence":"additional","affiliation":[{"name":"Center for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Santiago University Campus, 3810-193 Aveiro, Portugal"}]},{"given":"Tigressa Helena Soares","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Center for Exact Sciences and Technology, Vale do Acara\u00fa University, Sobral 62040-370, Brazil"}]},{"given":"Jane Eire Silva Alencar de","family":"Menezes","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"}]},{"given":"H\u00e9lcio Silva dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural Sciences, Cear\u00e1 State University, Fortaleza 60714-903, Brazil"},{"name":"Center for Exact Sciences and Technology, Vale do Acara\u00fa University, Sobral 62040-370, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Salmer\u00f3n-Manzano, E., Garrido-Cardenas, J.A., and Manzano-Agugliaro, F. 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