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This review initially presents the main principles of Raman spectroscopy and then its most relevant applications in the biomedical field, exploring the main advantages, challenges, and limitations. Additionally, other Raman-based techniques are identified as alternatives to the conventional technique. Overall, this review aims to present the currently available applications of Raman spectroscopy in the biomedical field and future appropriate perspectives, as possible guidance for new Raman-based biomedical devices.<\/jats:p>","DOI":"10.3390\/photochem5040029","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T14:50:38Z","timestamp":1758898238000},"page":"29","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biomedical Applications of Raman Spectroscopy: A Review"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6061-320X","authenticated-orcid":false,"given":"Sara","family":"Pimenta","sequence":"first","affiliation":[{"name":"CMEMS-UMinho, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory, Braga\/Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5991-1069","authenticated-orcid":false,"given":"Jos\u00e9 H.","family":"Correia","sequence":"additional","affiliation":[{"name":"CMEMS-UMinho, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory, Braga\/Guimar\u00e3es, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Khristoforova, Y., Bratchenko, L., and Bratchenko, I. 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