{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T04:08:37Z","timestamp":1773720517599,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Economic Area Financial Mechanism (MFEEE 2014\u20132021)","award":["PT-INNOVATION-0077"],"award-info":[{"award-number":["PT-INNOVATION-0077"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT) under the CEECIND program","award":["PT-INNOVATION-0077"],"award-info":[{"award-number":["PT-INNOVATION-0077"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phycology"],"abstract":"<jats:p>Ascophyllum nodosum is an ecologically and economically important species forming marine forests in temperate regions. In Europe, this brown seaweed reaches its southern distribution limit in the north of Portugal, where populations are under climatic pressure. Conservation and restoration actions are essential to preserve the important ecological roles of these populations, including biodiversity enhancement. In this study, we assessed the effect of temperature and light on the development of the early life stages of A. nodosum, from gamete germination and rhizoid development to germling growth, in order to support the establishment of nursery protocols for producing seedlings that can be used in reforestation actions. We found that for this population, temperature around 12 \u00b0C and low-light conditions (40 \u03bcmol m\u22122 s\u22121) favor gamete germination, rhizoid development, and initial germling growth during the first month, after which higher light supply (&gt;80 \u03bcmol m\u22122 s\u22121) is needed to sustain further growth stages. The results obtained in this study are relevant for the establishment of nursery methods for A. nodosum and should be complemented by further studies to determine more precisely the light and nutrient requirements to optimize growth in the germlings\u2019 later growth stages.<\/jats:p>","DOI":"10.3390\/phycology5010004","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T06:46:10Z","timestamp":1737009970000},"page":"4","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Influence of Light Intensity and Temperature on the Development of Early Life Stages of Ascophyllum nodosum (Phaeophyceae)"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1971-835X","authenticated-orcid":false,"given":"Ana","family":"Coutinho","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"S\u00edlvia","family":"Chemello","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8115-2134","authenticated-orcid":false,"given":"D\u00e9bora","family":"Borges","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6925-3805","authenticated-orcid":false,"given":"Francisco","family":"Arenas","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9231-0553","authenticated-orcid":false,"given":"Isabel","family":"Sousa-Pinto","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"FCUP\u2014Faculty of Sciences of the University of Porto, Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1528-3907","authenticated-orcid":false,"given":"Isabel","family":"Costa","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR-LA\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.seares.2017.02.004","article-title":"The Potential Role of Habitat-Forming Seaweeds in Modeling Benthic Ecosystem Properties","volume":"130","author":"Bustamante","year":"2017","journal-title":"J. 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