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Therefore, sustainable practices to help locals managing eucalyptus stands are needed. In this perspective, harnessing eucalyptus\u2019 specialized metabolism as a source of allelochemicals can be a promising approach for weed control. Thus, the main goals of this work were to evaluate the herbicidal potential of post-fire regenerated E. globulus leaves against Portulaca oleracea L. and to unravel the physiological mechanisms behind this phytotoxic action. For this, aqueous extracts of fresh (FLE; 617 g FW L\u22121) or oven-dried leaves (DLE; 250 g DW L\u22121) were foliar-sprayed at different dilutions in purslane seedlings. After five weeks, results revealed that DLE at the highest dose detained the greatest herbicidal activity, affecting purslane growth and cellular viability. Moreover, biochemical data pointed towards an overproduction of reactive oxygen species, causing harsh oxidative damage in roots, where the upregulation of important cellular players, like sugars, amino acids, and proline, was not able to reestablish redox homeostasis. Overall, this study proved that dried leaves from young E. globulus had potent herbicidal properties against P. oleracea and can represent a feasible strategy for weed management.<\/jats:p>","DOI":"10.3390\/plants10061159","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T22:23:00Z","timestamp":1623104580000},"page":"1159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Herbicidal Effects and Cellular Targets of Aqueous Extracts from Young Eucalyptus globulus Labill. Leaves"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6004-5786","authenticated-orcid":false,"given":"Mafalda","family":"Pinto","sequence":"first","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3330-2024","authenticated-orcid":false,"given":"Cristiano","family":"Soares","sequence":"additional","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"given":"Maria","family":"Martins","sequence":"additional","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4450-3596","authenticated-orcid":false,"given":"Bruno","family":"Sousa","sequence":"additional","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1438-1353","authenticated-orcid":false,"given":"In\u00eas","family":"Valente","sequence":"additional","affiliation":[{"name":"REQUIMTE, LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"},{"name":"REQUIMTE, LAQV, ICBAS, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6857-5968","authenticated-orcid":false,"given":"Ruth","family":"Pereira","sequence":"additional","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7756-5243","authenticated-orcid":false,"given":"Fernanda","family":"Fidalgo","sequence":"additional","affiliation":[{"name":"GreenUPorto-Sustainable Agrifood Production Research Centre, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,7]]},"reference":[{"key":"ref_1","first-page":"832","article-title":"Allelopathy for sustainable weed management","volume":"6","author":"Dahiya","year":"2017","journal-title":"J. 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