{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:55:48Z","timestamp":1778637348266,"version":"3.51.4"},"reference-count":81,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T00:00:00Z","timestamp":1635984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FEDER funding through COMPETE program","award":["POCI-01-0145-FEDER-016788"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016788"]}]},{"name":"Programa Operacional Regional de Lisboa-POR Lisboa","award":["LISBOA-01-0145-FEDER-016788"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-016788"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGR-PRO\/2183\/2014"],"award-info":[{"award-number":["PTDC\/AGR-PRO\/2183\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50017\/2020+UIDP\/50017\/2020"],"award-info":[{"award-number":["UIDB\/50017\/2020+UIDP\/50017\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020 and UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020 and UIDP\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/131766\/2017"],"award-info":[{"award-number":["SFRH\/BD\/131766\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The genus Lasiodiplodia has been reported from several grape growing regions and is considered as one of the fastest wood colonizers, causing Botryosphaeria dieback. The aim of this study was to (i) evaluate the efficacy of Esquive\u00ae, a biocontrol agent, on vineyard pruning wound protection, applied single or, in a combined protection strategy with a new site-targeted copper-based treatment (LC2017), and (ii) compare their efficacy with chemical protection provided by the commercially available product, Tessior\u00ae. For two seasons, protectants were applied onto pruning wounds, while LC2017 was applied throughout the season according to the manufacturer\u2019s instructions. Pruning wounds of two different cultivars were inoculated with three isolates of Lasiodiplodia spp. Efficacy of the wound protectants, varied between both years of the assay and according to the cultivar studied but were able to control the pathogen to some extent. The application of LC2017 did not show clear evidence of improving the control obtained by the sole application of the other products tested. Nevertheless, LC2017 showed a fungistatic effect against Lasiodiplodia spp., in vitro, and has previously shown an elicitor effect against grapevine trunk diseases. Therefore, this combination of two protection strategies may constitute a promising long-term approach to mitigate the impact of Botryosphaeria dieback.<\/jats:p>","DOI":"10.3390\/plants10112376","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T22:25:54Z","timestamp":1636064754000},"page":"2376","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Combining an HA + Cu (II) Site-Targeted Copper-Based Product with a Pruning Wound Protection Program to Prevent Infection with Lasiodiplodia spp. in Grapevine"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-7916","authenticated-orcid":false,"given":"Pedro","family":"Reis","sequence":"first","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"given":"Ana","family":"Gaspar","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0117-2958","authenticated-orcid":false,"given":"Artur","family":"Alves","sequence":"additional","affiliation":[{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-8812","authenticated-orcid":false,"given":"Florence","family":"Fontaine","sequence":"additional","affiliation":[{"name":"SFR Condorcet FR CNRS 3417, Universit\u00e9 de Reims Champagne-Ardenne, R\u00e9sistance Induite et Bioprotection des Plantes EA 4707, BP 1039, CEDEX 2, 51687 Reims, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-537X","authenticated-orcid":false,"given":"Cec\u00edlia","family":"Rego","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1094\/PDIS-04-17-0512-FE","article-title":"Managing grapevine trunk diseases with respects to etiology and epidemiology: Current strategies and future prospects","volume":"102","author":"Gramaje","year":"2017","journal-title":"Plant Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1365-3059.2012.02674.x","article-title":"Trunk diseases of grapevine: Complex and still poorly understood","volume":"62","author":"Bertsch","year":"2012","journal-title":"Plant Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.wep.2016.11.001","article-title":"Identifying economic hurdles to early adoption of preventative practices: The case of trunk diseases in California winegrape vineyards","volume":"5","author":"Kaplan","year":"2016","journal-title":"Wine Econ. 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