{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:13:53Z","timestamp":1760426033582,"version":"build-2065373602"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGR-TEC\/3555\/2012"],"award-info":[{"award-number":["PTDC\/AGR-TEC\/3555\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["PDR2020-784-042734"],"award-info":[{"award-number":["PDR2020-784-042734"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"R&amp;D Unit &quot;GREEN-IT - Bioresources for  Sustainability&quot;","award":["UIDB\/04551\/2020","UIDP\/04551\/2020"],"award-info":[{"award-number":["UIDB\/04551\/2020","UIDP\/04551\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Common bean is a nutritious food legume widely appreciated by consumers worldwide. It is a staple food in Latin America, and a component of the Mediterranean diet, being an affordable source of protein with high potential as a gourmet food. Breeding for nutritional quality, including both macro and micronutrients, and meeting organoleptic consumers\u2019 preferences is a difficult task which is facilitated by uncovering the genetic basis of related traits. This study explored the diversity of 106 Portuguese common bean accessions, under two contrasting environments, to gain insight into the genetic basis of nutritional composition (ash, carbohydrates, fat, fiber, moisture, protein, and resistant starch contents) and protein quality (amino acid contents and trypsin inhibitor activity) traits through a genome-wide association study. Single-nucleotide polymorphism-trait associations were tested using linear mixed models accounting for the accessions\u2019 genetic relatedness. Mapping resolution to the gene level was achieved in 56% of the cases, with 102 candidate genes proposed for 136 genomic regions associated with trait variation. Only one marker-trait association was stable across environments, highlighting the associations\u2019 environment-specific nature and the importance of genotype \u00d7 environment interaction for crops\u2019 local adaptation and quality. This study provides novel information to better understand the molecular mechanisms regulating the nutritional quality in common bean and promising molecular tools to aid future breeding efforts to answer consumers\u2019 concerns.<\/jats:p>","DOI":"10.3390\/plants11010026","type":"journal-article","created":{"date-parts":[[2021,12,23]],"date-time":"2021-12-23T02:01:19Z","timestamp":1640224879000},"page":"26","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Portuguese Common Bean Natural Variation Helps to Clarify the Genetic Architecture of the Legume\u2019s Nutritional Composition and Protein Quality"],"prefix":"10.3390","volume":"11","author":[{"given":"Francisco A.","family":"Mendes","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6483-0455","authenticated-orcid":false,"given":"Susana T.","family":"Leit\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Ver\u00f3nica","family":"Correia","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, 1649-019 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2340-6244","authenticated-orcid":false,"given":"Elsa","family":"Mecha","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-8616","authenticated-orcid":false,"given":"Diego","family":"Rubiales","sequence":"additional","affiliation":[{"name":"Instituto de Agricultura Sostenible, CSIC, Av. Men\u00e9ndez Pidal, 14004 Cordova, Spain"}]},{"given":"Maria Ros\u00e1rio","family":"Bronze","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, 1649-019 Lisboa, Portugal"},{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8469-7508","authenticated-orcid":false,"given":"Maria Carlota","family":"Vaz Patto","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.foodres.2009.09.003","article-title":"Pulse Proteins: Processing, Characterization, Functional Properties and Applications in Food and Feed","volume":"43","author":"Boye","year":"2010","journal-title":"Food Res. 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