{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T22:38:04Z","timestamp":1767998284473,"version":"3.49.0"},"reference-count":70,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"MICINN","doi-asserted-by":"publisher","award":["AGL2016-76143-C4-4-R and PID2020-112627RB-C33"],"award-info":[{"award-number":["AGL2016-76143-C4-4-R and PID2020-112627RB-C33"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["postdoctoral fellowship SFRH\/BPD\/108653\/2015 and the work contract 2477 of the con-tract-program 12345\/20181 defined by DL nr. 57\/2016, as well as through research funds awarded to RM (PTDC\/BIA-FBT\/28170\/2017) and to BioISI (UIDB\/04046\/2020 and UIDP\/04046"],"award-info":[{"award-number":["postdoctoral fellowship SFRH\/BPD\/108653\/2015 and the work contract 2477 of the con-tract-program 12345\/20181 defined by DL nr. 57\/2016, as well as through research funds awarded to RM (PTDC\/BIA-FBT\/28170\/2017) and to BioISI (UIDB\/04046\/2020 and UIDP\/04046"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Holm oak is a key tree species in Mediterranean ecosystems, whose populations have been increasingly threatened by oak decline syndrome, a disease caused by the combined action of Phytophthora cinnamomi and abiotic stresses. The aim of the present study was to produce holm oak plants that overexpress the Ginkbilobin-2 homologous domain gene (Cast_Gnk2-like) that it is known to possess antifungal properties. Proembryogenic masses (PEMs) isolated from four embryogenic lines (Q8, E2, Q10-16 and E00) were used as target explants. PEMs were co-cultured for 5 days with Agrobacterium EHA105pGnk2 and then cultured on selective medium containing kanamycin (kan) and carbenicillin. After 14 weeks on selective medium, the transformation events were observed in somatic embryos of lines Q8 and E2 and a total of 4 transgenic lines were achieved. The presence of the Cast_Gnk2-like gene on transgenic embryos was verified by PCR, and the number of transgene copies and gene expression was estimated by qPCR. Transgenic plants were obtained from all transgenic lines after cold storage of the somatic embryos for 2 months and subsequent transfer to germination medium. In an in vitro tolerance assay with the pathogen P. cinnamomi, we observed that transgenic plants were able to survive longer than wild type.<\/jats:p>","DOI":"10.3390\/plants11030304","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T21:07:11Z","timestamp":1643144831000},"page":"304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genetic Transformation of Quercus ilex Somatic Embryos with a Gnk2-like Protein That Reveals a Putative Anti-Oomycete Action"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8864-0277","authenticated-orcid":false,"given":"Susana","family":"Serrazina","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias, BioISI\u2014Biosystems & Integrative Sciences Institute, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"given":"M\u00aa Teresa","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Misi\u00f3n Biol\u00f3gica de Galicia, Consejo Superior de Investigaciones Cient\u00edficas (MBG-CSIC), Avda Vigo s\/n, Campus Vida, Apartado 122, 15705 Santiago de Compostela, Spain"}]},{"given":"Vanesa","family":"Cano","sequence":"additional","affiliation":[{"name":"Misi\u00f3n Biol\u00f3gica de Galicia, Consejo Superior de Investigaciones Cient\u00edficas (MBG-CSIC), Avda Vigo s\/n, Campus Vida, Apartado 122, 15705 Santiago de Compostela, Spain"}]},{"given":"Rui","family":"Malh\u00f3","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, BioISI\u2014Biosystems & Integrative Sciences Institute, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-6126","authenticated-orcid":false,"given":"Rita Louren\u00e7o","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Avenida da Rep\u00fablica, Quinta do Marqu\u00eas, 2780-159 Oeiras, Portugal"},{"name":"Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2839-8244","authenticated-orcid":false,"given":"Elena","family":"Corredoira","sequence":"additional","affiliation":[{"name":"Misi\u00f3n Biol\u00f3gica de Galicia, Consejo Superior de Investigaciones Cient\u00edficas (MBG-CSIC), Avda Vigo s\/n, Campus Vida, Apartado 122, 15705 Santiago de Compostela, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.3390\/f2020486","article-title":"Emerging Diseases in European Forest Ecosystems and Responses in Society","volume":"2","author":"Stenlid","year":"2011","journal-title":"Forests"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/j.envpol.2016.01.075","article-title":"Global topics and novel approaches in the study of air pollution, climate change and forest ecosystems","volume":"213","author":"Sicard","year":"2016","journal-title":"Environ. 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