{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:37:25Z","timestamp":1768016245713,"version":"3.49.0"},"reference-count":61,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["DL57\/2016\/CP1382\/CT0024"],"award-info":[{"award-number":["DL57\/2016\/CP1382\/CT0024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UID\/AGR\/04129\/2019"],"award-info":[{"award-number":["UID\/AGR\/04129\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDP\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Comission, FP7-KBBE","award":["Grant agreement ID: 311775"],"award-info":[{"award-number":["Grant agreement ID: 311775"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Grapevine (Vitis vinifera L.) is one of the most economically important crops worldwide, especially due to the economic relevance of wine production. Abiotic stress, such as drought, may contribute to low yield, shifts in quality, and important economic loss. The predicted climate change phenomena point to warmer and dryer Mediterranean environmental conditions; as such, it is paramount to study the effects of abiotic stress on grapevine performance. Deficit irrigation systems are applied to optimize water use efficiency without compromising berry quality. In this research, the effect of two deficit irrigation strategies, sustained deficit irrigation (SDI) and regulated deficit irrigation (RDI), in the grape berry were assessed. The effects of different levels of drought were monitored in Touriga Nacional at key stages of berry development (pea size, v\u00e9raison, and full maturation) through RNA-Seq transcriptome analysis and by specific differentially expressed genes (DEGs) monitoring through RT-qPCR. Handy datasets were obtained by bioinformatics analysis of raw RNA-Seq results. The dominant proportion of transcripts was mostly regulated by development, with v\u00e9raison showing more upregulated transcripts. Results showed that primary metabolism is the functional category more severely affected under water stress. Almost all DEGs selected for RT-qPCR were significantly upregulated in full maturation and showed the highest variability at v\u00e9raison and the lowest gene expression values in the pea size stage.<\/jats:p>","DOI":"10.3390\/plants11060827","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:34:27Z","timestamp":1647812067000},"page":"827","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Developmental Regulation of Transcription in Touriga Nacional Berries under Deficit Irrigation"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7858-8567","authenticated-orcid":false,"given":"Lu\u00edsa C.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5832-0945","authenticated-orcid":false,"given":"Miguel J. N.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-886X","authenticated-orcid":false,"given":"David","family":"Fa\u00edsca-Silva","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"given":"David","family":"van der Kellen","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5940-3750","authenticated-orcid":false,"given":"Jo\u00e3o C.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8186-6724","authenticated-orcid":false,"given":"Ricardo","family":"Egipto","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"given":"Carlos M.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]},{"given":"Sara","family":"Am\u00e2ncio","sequence":"additional","affiliation":[{"name":"Linking Landscape, Environment, Agriculture and Food Research Centre (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1649-004 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"ref_1","unstructured":"(2021, October 20). 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Notes"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/6\/827\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:40:03Z","timestamp":1760136003000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/6\/827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,21]]},"references-count":61,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["plants11060827"],"URL":"https:\/\/doi.org\/10.3390\/plants11060827","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,21]]}}}