{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:23:24Z","timestamp":1767666204629,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006595","name":"the Romanian Ministry of Education and Research, CNCS-UEFISCDI","doi-asserted-by":"publisher","award":["PN-III-P1-1.1-PD-2019-0603"],"award-info":[{"award-number":["PN-III-P1-1.1-PD-2019-0603"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Hypericum perforatum L., also known as St. John\u2019s Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H.\u00a0perforatum L. and H. rochelii Griseb. &amp; Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.<\/jats:p>","DOI":"10.3390\/plants11202773","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T22:19:53Z","timestamp":1666217993000},"page":"2773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Unravelling Phytochemical and Bioactive Potential of Three Hypericum Species from Romanian Spontaneous Flora: H. alpigenum, H.\u00a0perforatum and H. rochelii"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8055-9215","authenticated-orcid":false,"given":"Mihai","family":"Babot\u0103","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"}]},{"given":"Oleg","family":"Frumuzachi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5848-8457","authenticated-orcid":false,"given":"Andrei","family":"Mocan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"},{"name":"Laboratory of Chromatography, Institute of Advanced Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania"}]},{"given":"Mircea","family":"T\u0103ma\u0219","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8744-7814","authenticated-orcid":false,"given":"Maria In\u00eas","family":"Dias","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio Associado para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha (SusTEC), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7523-1637","authenticated-orcid":false,"given":"Jos\u00e9","family":"Pinela","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio Associado para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha (SusTEC), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4159-1471","authenticated-orcid":false,"given":"Dejan","family":"Stojkovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d-National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7381-756X","authenticated-orcid":false,"given":"Marina","family":"Sokovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d-National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia"}]},{"given":"Alexandru Sabin","family":"B\u0103d\u0103r\u0103u","sequence":"additional","affiliation":[{"name":"Faculty of Environmental Sciences and Engineering, Babe\u0219-Bolyai University, 30, F\u00e2nt\u00e2nele Street, 400294 Cluj-Napoca, Romania"}]},{"given":"Gianina","family":"Cri\u0219an","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9050-5189","authenticated-orcid":false,"given":"Lillian","family":"Barros","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio Associado para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha (SusTEC), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6967-8982","authenticated-orcid":false,"given":"Ramona","family":"P\u0103ltinean","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, Romania"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.3389\/fpls.2016.01687","article-title":"Editorial: Molecular and Biotechnological Advancements in Hypericum Species","volume":"7","author":"Franklin","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_2","unstructured":"European Medicines Agency HMPC Community Herbal Monograph Hypericum Perforatum L (2022, January 08). Herba (Traditional Use). Available online: https:\/\/www.ema.europa.eu\/en\/documents\/herbal-monograph\/final-community-herbal-monograph-hypericum-perforatum-l-herba-traditional-use_en.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8388","DOI":"10.3390\/ijms12128388","article-title":"Enhanced Antiproliferative and Apoptotic Response of HT-29 Adenocarcinoma Cells to Combination of Photoactivated Hypericin and Farnesyltransferase Inhibitor Manumycin A","volume":"12","author":"Kello","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Allegra, A., Tonacci, A., Spagnolo, E.V., Musolino, C., and Gangemi, S. (2020). Antiproliferative Effects of St. John\u2019s Wort, Its Derivatives, and Other Hypericum Species in Hematologic Malignancies. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010146"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S1357-2725(01)00126-1","article-title":"Hypericin in cancer treatment: More light on the way","volume":"34","author":"Agostinis","year":"2002","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jpba.2018.03.048","article-title":"Methodological aspects of biologically active compounds quantification in the genus Hypericum","volume":"155","author":"Dresler","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_7","first-page":"1045","article-title":"Hypericum perforatum: Traditional uses, clinical trials, and drug interactions","volume":"25","author":"Nobakht","year":"2022","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111735","DOI":"10.1016\/j.indcrop.2019.111735","article-title":"Anti-aging potential and anti-tyrosinase activity of three Hypericum species with focus on phytochemical composition by LC\u2013MS\/MS","volume":"141","author":"Ersoy","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"710","DOI":"10.2174\/1389557515666150709105844","article-title":"New Potential Pharmaceutical Applications of Hypericum Species","volume":"16","author":"Marrelli","year":"2016","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.2174\/0929867311320180001","article-title":"Do Other Hypericum Species Have Medical Potential As St. John\u2019s Wort (Hypericum perforatum)?","volume":"20","author":"Stojanovic","year":"2013","journal-title":"Curr. Med. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15422119.2020.1797792","article-title":"Hypericin: Source, Determination, Separation, and Properties","volume":"51","author":"Zhang","year":"2022","journal-title":"Sep. Purif. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.11646\/phytotaxa.4.1.2","article-title":"Studies in the genus Hypericum L. (Hypericaceae) 5(1). Sections 10. Olympia to 15\/16. Crossophyllum","volume":"4","author":"Robson","year":"2013","journal-title":"Phytotaxa"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/S0305-1978(98)00010-6","article-title":"Mangiferin and isomangiferin in some Hypericum species","volume":"26","author":"Kitanov","year":"1998","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"26","DOI":"10.5937\/savteh1902026Q","article-title":"Chemical composition of Hypericum rochelii Griseb. &Schenk headspace volatiles","volume":"8","year":"2019","journal-title":"Adv. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jpba.2013.01.024","article-title":"The case of Hypericum rochelii Griseb. & Schenk and Hypericum umbellatum A. Kern. essential oils: Chemical composition and antimicrobial activity","volume":"77","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_16","unstructured":"(2022, January 08). Euromed+ PlantBase Hypericum richeri Vill\u2014Euromed+ PlantBase. Available online: https:\/\/europlusmed.org\/cdm_dataportal\/taxon\/3f2d7da8-b87f-4f41-9b79-b4bbc7d96257."},{"key":"ref_17","first-page":"93","article-title":"Revision of the genus Hypericum L. (Hypericaceae) in three herbarium collections from Serbia","volume":"5","author":"Zlatkovic","year":"2014","journal-title":"Bull. Nat. Hist. Mus."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/ffj.1412","article-title":"Essential oil composition of Hypericum richeri Vill. from Italy","volume":"20","author":"Ferretti","year":"2005","journal-title":"Flavour Fragr. J."},{"key":"ref_19","first-page":"155","article-title":"Secondary metabolites of Hypericum richeri Vill. collected in Central Italy: Chemotaxonomy and ethnomedicinal relevance","volume":"2","author":"Venditti","year":"2018","journal-title":"Trends Phytochem. Res."},{"key":"ref_20","first-page":"1934578X1701201","article-title":"Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties","volume":"12","author":"Zdunic","year":"2017","journal-title":"Nat. Prod. Commun."},{"key":"ref_21","first-page":"1215","article-title":"Anti-inflammatory and gastroprotective properties of Hypericum richeri oil extracts","volume":"5","author":"Godevac","year":"2010","journal-title":"Nat. Prod. Commun."},{"key":"ref_22","first-page":"5486","article-title":"A detailed chemical composition and antimicrobial activity of Hypericum richeri Vill. subsp. grisebachii (Boiss.) Nyman essential oil from Serbia","volume":"5","year":"2011","journal-title":"J. Med. Plant Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1021\/jf026187q","article-title":"Hierarchical scheme for LC-MSn identification of chlorogenic acids","volume":"51","author":"Clifford","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/pca.935","article-title":"Profiling the chlorogenic acids of aster by HPLC-MSn","volume":"17","author":"Clifford","year":"2006","journal-title":"Phytochem. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.foodres.2016.08.040","article-title":"Bioactive properties and functional constituents of Hypericum androsaemum L.: A focus on the phenolic profile","volume":"89","author":"Jabeur","year":"2016","journal-title":"Food Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.1016\/j.foodchem.2013.06.109","article-title":"Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment","volume":"141","author":"Du","year":"2013","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1007\/s11696-021-01940-0","article-title":"UHPLC\u2013DAD\u2013ESI\u2013MS\/MS characterization of St. John\u2019s wort infusions from Serbia origin","volume":"76","year":"2022","journal-title":"Chem. Pap."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/pca.2416","article-title":"Qualitative and Quantitative Analysis of Phenolic Compounds in the Leaves of Aquilaria sinensis Using Liquid Chromatography-Mass Spectrometry","volume":"24","author":"Yu","year":"2013","journal-title":"Phytochem. Anal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1007\/s12161-016-0457-y","article-title":"Quantitation by HPLC-UV of Mangiferin and Isomangiferin in Coffee (Coffea arabica) Leaves from Brazil and Costa Rica After Solvent Extraction and Infusion","volume":"9","author":"Trevisan","year":"2016","journal-title":"Food Anal. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1590\/S0102-695X2006000300009","article-title":"Determination of myricetin derivatives in Chrysobalanus icaco L. (Chrysobalanaceae)","volume":"16","author":"Barbosa","year":"2006","journal-title":"Rev. Bras. Farmacogn."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1007\/s00216-020-02547-2","article-title":"Profiling of quercetin glycosides and acyl glycosides in sun-dried peperoni di Senise peppers (Capsicum annuum L.) by a combination of LC-ESI(-)-MS\/MS and polarity prediction in reversed-phase separations","volume":"412","author":"Pascale","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ijms.2005.09.011","article-title":"A comparison of flavonoid glycosides by electrospray tandem mass spectrometry","volume":"248","author":"March","year":"2006","journal-title":"Int. J. Mass Spectrom."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1080\/14786410802401390","article-title":"Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity","volume":"25","year":"2011","journal-title":"Nat. Prod. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.sajb.2015.09.022","article-title":"Secondary metabolites of Hypericum species from the Drosanthe and Olympia sections","volume":"104","author":"Cirak","year":"2016","journal-title":"S. Afr. J. Bot."},{"key":"ref_35","first-page":"1","article-title":"Taxonomy and Chemotaxonomy of the Genus Hypericum","volume":"5","author":"Crockett","year":"2011","journal-title":"Med. Aromat. Plant Sci. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1021\/jf072657a","article-title":"HPLC-DAD-MS\/MS-ESI screening of phenolic compounds in Pieris brassicae L. Reared on brassica rapa var. rapa L.","volume":"56","author":"Ferreres","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1039\/C6FO01042C","article-title":"Wild Fragaria vesca L. fruits: A rich source of bioactive phytochemicals","volume":"7","author":"Dias","year":"2016","journal-title":"Food Funct."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.indcrop.2014.10.021","article-title":"Phenolic profile and antioxidant properties of commercial and wild Fragaria vesca L. roots: A comparison between hydromethanolic and aqueous extracts","volume":"63","author":"Dias","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.indcrop.2016.04.065","article-title":"Phenolic profile and antioxidant activity of Coleostephus myconis (L.) Rchb.f.: An underexploited and highly disseminated species","volume":"89","author":"Bessada","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.indcrop.2015.11.018","article-title":"Antioxidant potential of two Apiaceae plant extracts: A comparative study focused on the phenolic composition","volume":"79","author":"Martins","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.foodchem.2012.08.025","article-title":"Nutrients, phytochemicals and bioactivity of wild Roman chamomile: A comparison between the herb and its preparations","volume":"136","author":"Barros","year":"2013","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.foodres.2012.07.046","article-title":"Crataegus monogyna buds and fruits phenolic extracts: Growth inhibitory activity on human tumor cell lines and chemical characterization by HPLC-DAD-ESI\/MS","volume":"49","author":"Rodrigues","year":"2012","journal-title":"Food Res. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105954","DOI":"10.1016\/j.ultsonch.2022.105954","article-title":"Optimized ultrasound-assisted extraction of phenolic compounds from Thymus comosus Heuff. ex Griseb. et Schenk (wild thyme) and their bioactive potential","volume":"84","author":"Frumuzachi","year":"2022","journal-title":"Ultrason. Sonochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1080\/13880209.2016.1270973","article-title":"Bioactive compounds from Hypericum humifusum and Hypericum perfoliatum: Inhibition potential of polyphenols with acetylcholinesterase and key enzymes linked to type-2 diabetes","volume":"55","author":"Rokbeni","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.foodchem.2006.05.062","article-title":"Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans","volume":"103","author":"Spiteller","year":"2007","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.jep.2010.07.034","article-title":"A review of the antibacterial activity of Hypericum perforatum L.","volume":"131","author":"Saddiqe","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.sajb.2020.05.018","article-title":"Phytochemical profile, antioxidant and antibacterial activity of four Hypericum species from the UK","volume":"133","author":"Saddiqe","year":"2020","journal-title":"S. Afr. J. Bot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.trac.2016.03.010","article-title":"Chemical perspective and criticism on selected analytical methods used to estimate the total content of phenolic compounds in food matrices","volume":"80","author":"Granato","year":"2016","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.foodchem.2018.04.012","article-title":"Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods?","volume":"264","author":"Granato","year":"2018","journal-title":"Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"305","DOI":"10.37358\/RC.18.2.6095","article-title":"HPLC screening of bioactives compounds and antioxidant capacity of different hypericum species","volume":"69","author":"Gitea","year":"2018","journal-title":"Rev. Chim."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.indcrop.2012.07.017","article-title":"Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L. (St. John\u2019s wort)","volume":"43","author":"Altun","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1039\/C8NJ00347E","article-title":"Phytochemical characterization, in vitro and in silico approaches for three Hypericum species","volume":"42","author":"Zengin","year":"2018","journal-title":"New J. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.sajb.2018.05.030","article-title":"Phenolic profile and biological activity of Hypericum perforatum L.: Can roots be considered as a new source of natural compounds?","volume":"117","author":"Tusevski","year":"2018","journal-title":"S. Afr. J. Bot."},{"key":"ref_54","first-page":"1036","article-title":"Evaluation of Chemical Composition and In-vitro Biological Activities of Three Endemic Hypericum Species from Anatolia (H. thymbrifolium, H. spectabile and H. pseudolaeve)","volume":"17","author":"Ozkan","year":"2018","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.jep.2019.02.020","article-title":"Anti-quorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa","volume":"235","author":"Sesal","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"101061","DOI":"10.1016\/j.eujim.2020.101061","article-title":"The antibacterial and antivirulent potential of Hypericum lydium against Staphylococcus aureus: Inhibition of growth, biofilm formation, and hemolytic activity","volume":"35","year":"2020","journal-title":"Eur. J. Integr. Med."},{"key":"ref_57","first-page":"634","article-title":"Chemical Composition, Diuretic, and Antityrosinase Activity of Traditionally Used Romanian Cerasorum stipites","volume":"12","author":"Mihali","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.eujim.2018.08.004","article-title":"Amylase, glucosidase, tyrosinase, and cholinesterases inhibitory, antioxidant effects, and GC-MS analysis of wild mint (Mentha longifolia var. calliantha) essential oil: A natural remedy","volume":"22","author":"Asghari","year":"2018","journal-title":"Eur. J. Integr. Med."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Kamoutsis, C., Fesatidou, M., Petrou, A., Geronikaki, A., Poroikov, V., Ivanov, M., Sokovi\u0107, M., \u0106iri\u0107, A., Carazo, A., and Mlad\u011bnka, P. (2021). Triazolo Based-Thiadiazole Derivatives. Synthesis, Biological Evaluation and Molecular Docking Studies. Antibiotics, 10.","DOI":"10.3390\/antibiotics10070804"},{"key":"ref_60","unstructured":"(2020). RStudio: Integrated Development for R. Rstudio Team, PBC. Available online: http:\/\/www.rstudio.com."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/20\/2773\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:57:41Z","timestamp":1760144261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/20\/2773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,19]]},"references-count":60,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["plants11202773"],"URL":"https:\/\/doi.org\/10.3390\/plants11202773","relation":{},"ISSN":["2223-7747"],"issn-type":[{"type":"electronic","value":"2223-7747"}],"subject":[],"published":{"date-parts":[[2022,10,19]]}}}