{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T09:12:05Z","timestamp":1778317925997,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T00:00:00Z","timestamp":1669766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007364","name":"Fondazione CRT","doi-asserted-by":"publisher","award":["RF.2018.2549"],"award-info":[{"award-number":["RF.2018.2549"]}],"id":[{"id":"10.13039\/100007364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Castanea sativa cv. \u2018Garrone Rosso\u2019 and \u2018Marrone di Castel del Rio\u2019 are two of the most prized varieties in Italy due to their valuable and healthy nuts used for fresh consumption and in the confectionery industry. Despite the growing demand for chestnuts, there are constraints regarding plant propagation that hamper the renewal and new planting of orchards in different areas. Castanea sativa is susceptible to diseases that have caused a reduction in its area of production. For this reason, in vitro culture represents a valuable technique for germplasm preservation and plant multiplication enabling production of a high number of plants for use in breeding programs. Here we present an in vitro micropropagation protocol for Italian Castanea sativa cv. \u2018Marrone di Castel del Rio\u2019 and cv. \u2018Garrone Rosso\u2019 to contribute to the preservation and enhancement of the Italian germplasm. Nodal explants were used as the starting material for in vitro establishment. The cv. \u2018Marrone di Castel del Rio\u2019 showed a high percentage of survival explants (92%) when subjected to long bleach exposure (25 min), in contrast to what was observed for the \u2018Garrone Rosso\u2019 cultivar. Ascorbic acid was found to be the best compound to counteract phenol exudation. The MS3B and DKW media supplied with 0.5 mg\/L BAP were effective for in vitro establishment, while the DKW medium (0.1 mg\/L BAP and 0.05 mg\/L IBA) was preferable for the proliferation phase. A double-layer rooting methodology was used and 35% rooting was observed with 25 mg\/L IBA rooting treatment.<\/jats:p>","DOI":"10.3390\/plants11233308","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T03:49:13Z","timestamp":1669780153000},"page":"3308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6863-3950","authenticated-orcid":false,"given":"Vera","family":"Pavese","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Universit\u00e0 degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy"}]},{"given":"Paola","family":"Ruffa","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Universit\u00e0 degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy"}]},{"given":"Silvia","family":"Abb\u00e0","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Universit\u00e0 degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-6126","authenticated-orcid":false,"given":"Rita Louren\u00e7o","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria I.P., Avenida da Rep\u00fablica, Quinta do Marqu\u00eas, 2780-159 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2839-8244","authenticated-orcid":false,"given":"Elena","family":"Corredoira","sequence":"additional","affiliation":[{"name":"Misi\u00f3n Biol\u00f3gica de Galicia, Sede de Santiago de Compostela, Consejo Superior de Investigaciones Cient\u00edficas, Avd. Vigo s\/n, 15705 Santiago de Compostela, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9634-9586","authenticated-orcid":false,"given":"Cristian","family":"Silvestri","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie e Forestali (DAFNE), Universit\u00e0 della Tuscia, Via San Camillo De Lellis, 01100 Viterbo, Italy"}]},{"given":"Daniela","family":"Torello Marinoni","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Universit\u00e0 degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy"}]},{"given":"Roberto","family":"Botta","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Forestali e Alimentari-DISAFA, Universit\u00e0 degli Studi di Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fernandes, P., Tedesco, S., Vieira da Silva, I., Santos, C., Machado, H., and Louren\u00e7o Costa, R. 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