{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:43:40Z","timestamp":1776905020151,"version":"3.51.2"},"reference-count":22,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T00:00:00Z","timestamp":1670803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/ASP-PLA\/28963\/2017"],"award-info":[{"award-number":["PTDC\/ASP-PLA\/28963\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: Eruca sativa (L.) Cav., usually referred to as annual garden rocket, and Diplotaxis\u00a0tenuifolia (L.) DC. commonly named to as perennial wild rocket. E. sativa is used for human consumption since antiquity. However, the growing consumer preference for D. tenuifolia is being accompanied by the fast increase in its production area and commercialization of new cultivars. Nevertheless, the worldwide number of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which situation the project \u201cREMIRucula\u201d intends to contribute, establishing a germplasm collection at the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing (NGS) of the first genome assembly of D. tenuifolia and the identification of specific single sequence repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific DNA-markers for this species. A representative set of 87 D. tenuifolia and 3 E. sativa accessions were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The non-discrimination within six pairs and one trio of D. tenuifolia accessions is discussed.<\/jats:p>","DOI":"10.3390\/plants11243482","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T02:59:01Z","timestamp":1670900341000},"page":"3482","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of Wild Rocket (Diplotaxis tenuifolia (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers"],"prefix":"10.3390","volume":"11","author":[{"given":"Jo\u00e3o M.","family":"Reis","sequence":"first","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"},{"name":"CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Ricardo J.","family":"Pereira","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"},{"name":"CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-998X","authenticated-orcid":false,"given":"Paula S.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta do Marqu\u00eas, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4278-0415","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Leit\u00e3o","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"},{"name":"CHANGE\u2014Global Change and Sustainability Institute, Faculdade de Ci\u00eancias e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"820925","DOI":"10.3389\/fpls.2022.820925","article-title":"Seed Germination and Seed Bank Dynamics of Eruca sativa (Brassicaceae): A Weed on the Northeastern Edge of Tibetan Plateau","volume":"13","author":"Jia","year":"2022","journal-title":"Front. 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