{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:15:33Z","timestamp":1760148933115,"version":"build-2065373602"},"reference-count":65,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"national funds by FCT\/MCTES for CESAM","award":["UIDP\/50017\/2020","UIDB\/50017\/2020","LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020","UIDB\/50017\/2020","LA\/P\/0094\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Santolina impressa is an aromatic Asteraceae species endemic to Portugal, traditionally used for its anti-inflammatory properties. The aim of this study was to characterize S. impressa secretory structures, analyze the essential oil (EO) from the aerial organs, and evaluate its antiviral activity against herpes simplex viruses HSV-1 and HSV-2. Secretory structures were investigated by light and scanning microscopy, and the secretion was histochemically characterized. The EO from the aerial organs in full blooming was analyzed by gas chromatography with flame ionization detection and gas chromatography\u2013mass spectrometry. Antiviral assays were performed by direct contact with viral suspensions (virucidal effect), and in infected Vero E6 cells, at different time periods during the viral replication cycle. Two types of secretory structures were described, biseriate glandular trichomes and secretory ducts, producing an oleoresin and a resin rich in flavonoids, respectively. Fifty compounds were identified in S. impressa EO, accounting for 87% of the total constituents. Monoterpenes constituted the main EO fraction (82%), with \u03b2-pinene (13%) and \u03b2-phellandrene (10%) being their major components. The EO interacted with HSV-1 and HSV-2 in a dose-dependent manner, thereby inactivating both viral infections. The EO did not evidence a virucidal effect but inhibited the HSV-1 and HSV-2 infection in Vero cells in a dose-dependent manner. However, further studies are needed to investigate the mode of action in the replication cycle.<\/jats:p>","DOI":"10.3390\/plants12132391","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T02:30:51Z","timestamp":1687314651000},"page":"2391","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["New Reports on the Portuguese Endemic Species, Santolina impressa: Secretory Structures, Essential Oil Composition and Antiviral Activity"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3257-9777","authenticated-orcid":false,"given":"Ana Margarida","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"given":"Ana Rita","family":"Mendes","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0559-6344","authenticated-orcid":false,"given":"Maria Filomena","family":"Caeiro","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3239-3190","authenticated-orcid":false,"given":"Ana Cristina","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7439-5912","authenticated-orcid":false,"given":"Lia","family":"Ascens\u00e3o","sequence":"additional","affiliation":[{"name":"Centro de Estudos do Ambiente e do Mar (CESAM Lisboa), Faculdade de Ci\u00eancias da Universidade de Lisboa (FCUL), DBV, C2, Campo Grande, 1749-016 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1128\/AAC.00615-10","article-title":"Resistance of Herpes simplex viruses to nucleoside analogues: Mechanisms, prevalence, and management","volume":"55","author":"Piret","year":"2011","journal-title":"Antimicrob. 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