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Amongst a diversity of remediation technologies, phytoremediation is an environmentally friendly technology that, when coupling tolerant plants to selected rhizospheric microorganisms, can greatly stimulate HM decontamination of soils. Maize (Zea mays) is a plant with the reported capacity for HM exclusion from contaminated soil but also has energetic importance. In this study, Zea mays was coupled with Rhizophagus irregularis, an arbuscular mycorrhizal fungus (AMF), and Cupriavidus sp. strain 1C2, a plant growth-promoting rhizobacteria (PGPR), as a remediation approach to remove Cd and Zn from an industrial contaminated soil (1.2 mg Cd kg\u22121 and 599 mg Zn kg\u22121) and generate plant biomass, by contrast to the conservative development of the plant in an agricultural (with no metal pollution) soil. Biomass production and metal accumulation by Z. mays were monitored, and an increase in plant yield of ca. 9% was observed after development in the contaminated soil compared to the soil without metal contamination, while the plants removed ca. 0.77% and 0.13% of the Cd and Zn initially present in the soil. The resulting biomass (roots, stems, and cobs) was used for biogas generation in several biomethane (BMP) assays to evaluate the potential end purpose of the phytoremediation-resulting biomass. It was perceptible that the HMs existent in the industrial soil did not hinder the anaerobic biodegradation of the biomass, being registered biomethane production yields of ca. 183 and 178 mL of CH4 g\u22121 VS of the complete plant grown in non-contaminated and contaminated soils, respectively. The generation of biomethane from HM-polluted soils\u2019 phytoremediation-derived maize biomass represents thus a promising possibility to be a counterpart to biogas production in an increasingly challenging status of renewable energy necessities.<\/jats:p>","DOI":"10.3390\/plants12203623","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T07:22:32Z","timestamp":1697786552000},"page":"3623","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Potential of Bioaugmentation-Assisted Phytoremediation Derived Maize Biomass for the Production of Biomethane via Anaerobic Digestion"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3058-8285","authenticated-orcid":false,"given":"Ana M.","family":"Paulo","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2185-6401","authenticated-orcid":false,"given":"N\u00eddia S.","family":"Caetano","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"},{"name":"CIETI\/ISEP\u2014Centro de Inova\u00e7\u00e3o em Engenharia e Tecnologia Industrial\/Instituto Superior de Engenharia, Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8232-1155","authenticated-orcid":false,"given":"Ana P. G. C.","family":"Marques","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.envpol.2013.03.032","article-title":"Safe use of metal-contaminated agricultural land by cultivation of energy maize (Zea mays)","volume":"178","author":"Witters","year":"2013","journal-title":"Environ. Pollut."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1080\/15226514.2013.876967","article-title":"Using Contaminated Plants Involved in Phytoremediation for Anaerobic Digestion","volume":"17","author":"Cao","year":"2015","journal-title":"Int. J. 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