{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:39:17Z","timestamp":1774330757498,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PT2020 and EAFRD (European Agricultural Fund for Rural Development)","award":["UIDB\/00690\/2020"],"award-info":[{"award-number":["UIDB\/00690\/2020"]}]},{"name":"PT2020 and EAFRD (European Agricultural Fund for Rural Development)","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["UIDB\/00690\/2020"],"award-info":[{"award-number":["UIDB\/00690\/2020"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]},{"name":"FEDER","award":["UIDB\/00690\/2020"],"award-info":[{"award-number":["UIDB\/00690\/2020"]}]},{"name":"FEDER","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>In the northeast of Portugal, like in many parts of the world, most soils are acidic, which may hamper crop productivity. This study presents the findings of a factorial experiment on olive (Olea europaea L.) involving three factors: (i) soil type [schist (Sch) and granite (Gra)]; (ii) cultivars [Cobran\u00e7osa (Cob) and Arbequina (Arb)]; and (iii) fertilizer treatments [liming (CaCO3) plus magnesium (Mg) (LMg), phosphorus (P) application (+P), boron (B) application (+B), all fertilizing materials combined (Con+), and an untreated control (Con-)]. Dry matter yield (DMY) did not show significant differences between cultivars, but plants grown in schist soil exhibited significantly higher biomass compared to those in granite soil. Among the treatments, +B and Con+ resulted in the highest DMY (50.8 and 47.2 g pot\u22121, respectively), followed by +P (34.3 g pot\u22121) and Con- (28.6 g pot\u22121). Treatment LMg yielded significantly lower values (15.6 g pot\u22121) than Con-. LMg raised the pH above 7 (7.36), leading to a severe B deficiency. Although Con+ also raised the pH above 7 (7.48), it ranked among the most productive treatments for providing B. Therefore, when applying lime to B-poor sandy soils, moderate rates are advised to avoid inducing a B deficiency. Additionally, it seems prudent to apply B after lime application.<\/jats:p>","DOI":"10.3390\/plants12244161","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T06:53:16Z","timestamp":1702623196000},"page":"4161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["On Sandy, Boron-Poor Soils, Liming Induced Severe Boron Deficiency and Drastically Reduced the Dry Matter Yield of Young Olive Trees"],"prefix":"10.3390","volume":"12","author":[{"given":"Margarida","family":"Arrobas","sequence":"first","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"given":"Soraia","family":"Raimundo","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6954-8144","authenticated-orcid":false,"given":"Nuno","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-559X","authenticated-orcid":false,"given":"Jos\u00e9","family":"Moutinho-Pereira","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2482-7873","authenticated-orcid":false,"given":"Carlos Manuel","family":"Correia","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-1129","authenticated-orcid":false,"given":"Manuel \u00c2ngelo","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"},{"name":"Laborat\u00f3rio para a Sustentabilidade e Tecnologia em Regi\u00f5es de Montanha, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1146\/annurev.arplant.55.031903.141655","article-title":"How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency","volume":"55","author":"Kochian","year":"2004","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rengel, Z. (2003). Handbook of Soil Acidity, Marcel Dekker, Inc.","DOI":"10.1201\/9780203912317"},{"key":"ref_3","unstructured":"FAO, and ITPS (2015). Status of the World\u2019s Soil Resources (SWSR)\u2014Main Report, Food and Agriculture Organization of the United Nations and Intergovernmental Technical Panel on Soils."},{"key":"ref_4","unstructured":"Adams, F. (1984). Soil Acidity and Liming, American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc.. [2nd ed.]."},{"key":"ref_5","unstructured":"Weil, R.R., and Brady, N.C. (2017). Nature and Properties of Soils, Pearson. [15th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.scitotenv.2017.08.020","article-title":"Liming impacts on soils, crops and biodiversity in the UK: A review","volume":"610\u2013611","author":"Holland","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1093\/jexbot\/52.357.791","article-title":"Why plants grow poorly on very acid soils: Are ecologists missing the obvious?","volume":"52","author":"Kidd","year":"2001","journal-title":"J. Exp. Bot."},{"key":"ref_8","unstructured":"Upjohn, B., Fenton, G., and Conyers, M. (2005). Soil Acidity and Liming, Department of Primary Industries. [3rd ed.]. Agfact AC.19."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1111\/j.1475-2743.2008.00166.x","article-title":"Soil acidity and aluminium toxicity as affected by surface liming and cover oat residues under a no-till system","volume":"24","author":"Caires","year":"2008","journal-title":"Soil Use Manag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1111\/sum.12948","article-title":"Dolomitic limestone was more effective than calcitic limestone in increasing soil pH in an untilled olive orchard","volume":"39","author":"Arrobas","year":"2023","journal-title":"Soil Use Manag."},{"key":"ref_11","unstructured":"Agroconsultores, E.C. (1991). Carta dos Solos, Carta de Utiliza\u00e7\u00e3o Actual da Terra e Carta de Aptid\u00e3o da Terra do Nordeste de Portugal, Universidade de Tr\u00e1s-os-Montes e Alto Douro."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.scienta.2017.10.027","article-title":"Diagnosing the nutritional condition of chestnut groves by soil and leaf analyses","volume":"228","author":"Arrobas","year":"2018","journal-title":"Sci. Hortic."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lopes, J.I., Arrobas, M., Brito, C., Gon\u00e7alves, A., Silva, E., Martins, S., Raimundo, S., Rodrigues, M.A., and Correia, C.M. (2020). Mycorrhizal fungi were more effective than zeolites in increasing the growth of non-irrigated young olive trees planted in an acidic soil. Sustainability, 12.","DOI":"10.3390\/su122410630"},{"key":"ref_14","unstructured":"IPMA (2023, November 18). Normais Climatol\u00f3gicas; Instituto Nacional do Mar e da Atmosfera: 2023. Available online: https:\/\/www.ipma.pt\/pt\/oclima\/normais.clima\/."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e0901","DOI":"10.5424\/sjar\/2019171-13796","article-title":"Soil and foliar applied boron in olive: Tree crop growth and yield, and boron remobilization within plant tissue","volume":"17","author":"Ferreira","year":"2019","journal-title":"Span. J. Agric. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1080\/01904167.2011.580812","article-title":"Boron application on a chestnut orchard: Effect on yield and quality of nuts","volume":"34","author":"Portela","year":"2011","journal-title":"J. Plant Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1080\/01904167.2014.963116","article-title":"Assessment of boron application in chestnuts: Nut yield and quality","volume":"38","author":"Portela","year":"2015","journal-title":"J. Plant Nutr."},{"key":"ref_18","unstructured":"Sibbett, G.S., and Ferguson, L. (2005). Olive Production Manual, University of California Publication. [2nd ed.]."},{"key":"ref_19","unstructured":"Barranco, D., Fern\u00e1ndez-Escobar, R., and Rallo, L. (2017). El Cultivo del Olivo, Mundi Prensa. [7th ed.]."},{"key":"ref_20","unstructured":"Barranco, D., Fern\u00e1ndez-Escobar, R., and Rallo, L. (2017). El Cultivo del Olivo, Mundi Prensa. [7th ed.]."},{"key":"ref_21","unstructured":"Barranco, D., Fern\u00e1ndez-Escobar, R., and Rallo, L. (2017). El Cultivo del Olivo, Mundi Prensa. [7th ed.]."},{"key":"ref_22","unstructured":"Marschner, P. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Academic Press. [3rd ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s004680000056","article-title":"Leaf morphology and macronutrients in broadleaved trees","volume":"14","author":"Bussotti","year":"2000","journal-title":"Trees"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/j.1469-8137.2009.02830.x","article-title":"Causes and consequences of variation in leaf mass per area (LMA): A meta-analysis","volume":"182","author":"Poorter","year":"2009","journal-title":"New Phytol."},{"key":"ref_25","first-page":"497","article-title":"Relationships between leaf mass per area and nutrient concentrations in 98 Mediterranean woody species are determined by phylogeny, habitat and leaf habit","volume":"42","author":"Villar","year":"2018","journal-title":"Trees"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/treephys\/24.2.233","article-title":"Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions","volume":"24","author":"Bacelar","year":"2004","journal-title":"Tree Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lopes, J.I., Correia, C.M., Gon\u00e7alves, A., Silva, E., Martins, S., Arrobas, M., and Rodrigues, M.A. (2021). Arbuscular mycorrhizal fungi inoculation reduced the growth of pre-rooted olive cuttings in a greenhouse. Soil Syst., 5.","DOI":"10.3390\/soilsystems5020030"},{"key":"ref_28","unstructured":"Marschner, P. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Academic Press. [3rd ed.]."},{"key":"ref_29","unstructured":"Santos, J.Q. (2015). Fertiliza\u00e7\u00e3o, Fundamentos Agroambientais da Utiliza\u00e7\u00e3o dos Adubos e Corretivos, Publind\u00fastria."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"300","DOI":"10.3389\/fpls.2020.00300","article-title":"Manganese in plants: From acquisition to subcellular allocation","volume":"11","author":"Alejandro","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11738-016-2165-z","article-title":"Effects of magnesium deficiency on photosynthesis and carbohydrate partitioning","volume":"38","author":"Farhat","year":"2016","journal-title":"Acta Physiol. Plant"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hassan, M.U., Aamer, M., Chattha, M.U., Haiying, T., Shahzad, B., Barbanti, L., Nawaz, M., Rasheed, A., Afzal, A., and Liu, Y. (2020). The critical role of zinc in plants facing the drought stress. Agriculture, 10.","DOI":"10.3390\/agriculture10090396"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0065-2113(08)60887-1","article-title":"The dilution effect in plant nutrition studies","volume":"34","author":"Jarrell","year":"1981","journal-title":"Adv. Agron."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1128\/microbiolspec.FUNK-0012-2016","article-title":"The mutualistic interaction between plants and arbuscular mycorrhizal fungi","volume":"4","author":"Lanfranco","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.soilbio.2008.11.013","article-title":"Organic acid exudation by mycorrhizal Andropogon virginicus L. (broomsedge) roots in response to aluminum","volume":"41","author":"Cumming","year":"2009","journal-title":"Soil. Biol. Biochem."},{"key":"ref_36","unstructured":"Bryson, G.M., Mills, H.A., Sasseville, D.N., Jones, J.B., and Barker, A.V. (2014). Plant Analysis Handbook III. A Guide to Sampling, Preparation, Analysis and Interpretation for Agronomic and Horticultural Crops, Micro-Macro Publishing, Inc."},{"key":"ref_37","unstructured":"Barker, A.V., and Pilbeam, D.J. (2007). Handbook of Plant Nutrition, CRC Press."},{"key":"ref_38","unstructured":"Marschner, P. (2012). Marschner\u2019s Mineral Nutrition of Higher Plants, Academic Press. [3rd ed.]."},{"key":"ref_39","unstructured":"Sibbett, G.S., and Ferguson, L. (2005). Olive Production Manual, University of California Publication. [2nd ed.]."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.envexpbot.2006.10.003","article-title":"Changes in growth, gas exchange, xylem hydrualic properties and water use efficiency of three olive cultivars under contrasting water availability regimes","volume":"60","author":"Bacelar","year":"2007","journal-title":"Environ. Exp. Bot."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1016\/j.jplph.2007.11.002","article-title":"Boron deficiency decreases growth and photosynthesis, and increases starch and hexoses in leaves of citrus seedlings","volume":"165","author":"Han","year":"2008","journal-title":"J. Plant Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s13580-015-0044-y","article-title":"Boron availability alters its distribution in plant parts of tomato","volume":"56","author":"Choi","year":"2015","journal-title":"Hortic. Environ. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4420","DOI":"10.1038\/s41598-017-04655-z","article-title":"Effect of boron deficiency on anatomical structure and chemical composition of petioles and photosynthesis of leaves in cotton (Gossypium hirsutum L.)","volume":"7","author":"Li","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wei, R., Huang, M., Huang, D., Zhou, J., Pan, X., and Zhang, W. (2022). Growth, gas exchange, and boron distribution characteristics in two grape species plants under boron deficiency condition. Horticulturae, 8.","DOI":"10.3390\/horticulturae8050374"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Lopes, J.I., Gon\u00e7alves, A., Brito, C., Martins, S., Pinto, L., Moutinho-Pereira, J., Raimundo, S., Arrobas, M., Rodrigues, M.A., and Correia, C.M. (2021). Inorganic fertilization at high N rate increased olive yield of a rainfed orchard but reduced soil organic matter in comparison to three organic amendments. Agronomy, 11.","DOI":"10.3390\/agronomy11112172"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.scienta.2018.11.055","article-title":"Significance of proper nitrogen fertilization for olive productivity in intensive cultivation","volume":"246","author":"Haberman","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1111\/j.1365-3040.1994.tb02028.x","article-title":"A theoretical explanation of the Piper-Steenbjerg effect","volume":"17","year":"1994","journal-title":"Plant Cell Environ."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"428","DOI":"10.5274\/jsbr.16.5.428","article-title":"Boron nutrition in the growth and sugar content of sugarbeets","volume":"16","author":"Vlamis","year":"1970","journal-title":"Amer. Soc. Sugar Beet. Technol. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1023\/A:1004211925160","article-title":"Boron mobility in plants","volume":"193","author":"Brown","year":"1997","journal-title":"Plant Soil"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"616","DOI":"10.21273\/HORTSCI.29.6.616","article-title":"Mobilization of boron in olive trees during flowering and fruit development","volume":"29","author":"Delgado","year":"1994","journal-title":"HortScience"},{"key":"ref_51","unstructured":"WRB (2015). World Reference Base for Soil Resources 2014, Update 2015. International Soil Classification System for Naming Soils and Creating Legends for Soil Maps, FAO. World Soil Resources Reports No. 106."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/BF00384257","article-title":"Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves","volume":"153","author":"Farquhar","year":"1981","journal-title":"Planta"},{"key":"ref_53","unstructured":"Van Reeuwijk, L.P. (2002). Procedures for Soil Analysis, FAO (Food and Agriculture Organization of the United Nations). [6th ed.]. Technical Paper 9."},{"key":"ref_54","unstructured":"FAO (2022). Standard Operating Procedure for Soil Available Micronutrients (Cu, Fe, Mn, Zn) and Heavy Metals (Ni, Pb, Cd), DTPA Extraction Method."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Temminghoff, E.E., and Houba, V.J. (2004). Plant Analysis Procedures, Kluwer Academic Publishers. [2nd ed.].","DOI":"10.1007\/978-1-4020-2976-9"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/24\/4161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:38:43Z","timestamp":1760132323000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/24\/4161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,14]]},"references-count":55,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["plants12244161"],"URL":"https:\/\/doi.org\/10.3390\/plants12244161","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,14]]}}}