{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:28:29Z","timestamp":1768465709858,"version":"3.49.0"},"reference-count":68,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T00:00:00Z","timestamp":1709683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Center for Functional Ecology\u2014Science for People and the Planet","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"Center for Functional Ecology\u2014Science for People and the Planet","award":["IN607A2021\/06"],"award-info":[{"award-number":["IN607A2021\/06"]}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["IN607A2021\/06"],"award-info":[{"award-number":["IN607A2021\/06"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT\/MCTES","award":["UIDB\/04004\/2020"],"award-info":[{"award-number":["UIDB\/04004\/2020"]}]},{"name":"FCT\/MCTES","award":["IN607A2021\/06"],"award-info":[{"award-number":["IN607A2021\/06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Phase change refers to the process of maturation and transition from the juvenile to the adult stage. In response to this shift, certain species like chestnut lose the ability to form adventitious roots, thereby hindering the successful micropropagation of adult plants. While auxin is the main hormone involved in adventitious root formation, other hormones, such as ethylene, are also thought to play a role in its induction and development. In this study, experiments were carried out to determine the effects of ethylene on the induction and growth of adventitious roots. The analysis was performed in two types of chestnut microshoots derived from the same tree, a juvenile-like line with a high rooting ability derived from basal shoots (P2BS) and a line derived from crown branches (P2CR) with low rooting responses. By means of the application of compounds to modify ethylene content or inhibit its signalling, the potential involvement of this hormone in the induction of adventitious roots was analysed. Our results show that ethylene can modify the rooting competence of mature shoots, while the response in juvenile material was barely affected. To further characterise the molecular reasons underlying this maturation-derived shift in behaviour, specific gene expression analyses were developed. The findings suggest that several mechanisms, including ethylene signalling, auxin transport and epigenetic modifications, relate to the modulation of the rooting ability of mature chestnut microshoots and their recalcitrant behaviour.<\/jats:p>","DOI":"10.3390\/plants13050738","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T05:48:01Z","timestamp":1709704081000},"page":"738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ethylene Action Inhibition Improves Adventitious Root Induction in Adult Chestnut Tissues"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1525-935X","authenticated-orcid":false,"given":"Ricardo","family":"Castro-Camba","sequence":"first","affiliation":[{"name":"Department of Plant Production, Misi\u00f3n Biol\u00f3gica de Galicia, CSIC, Avda de Vigo s\/n, 15705 Santiago de Compostela, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6395-793X","authenticated-orcid":false,"given":"Mariana","family":"Neves","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2151-3916","authenticated-orcid":false,"given":"Sandra","family":"Correia","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"InnovPlantProtect CoLab, Estrada de Gil Vaz, 7350-478 Elvas, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2299-298X","authenticated-orcid":false,"given":"Jorge","family":"Canhoto","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1367-535X","authenticated-orcid":false,"given":"Jes\u00fas M.","family":"Vielba","sequence":"additional","affiliation":[{"name":"Department of Plant Production, Misi\u00f3n Biol\u00f3gica de Galicia, CSIC, Avda de Vigo s\/n, 15705 Santiago de Compostela, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1304-0736","authenticated-orcid":false,"given":"Conchi","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Plant Production, Misi\u00f3n Biol\u00f3gica de Galicia, CSIC, Avda de Vigo s\/n, 15705 Santiago de Compostela, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s11295-011-0427-x","article-title":"Landscape genetic structure of chestnut (Castanea sativa Mill.) in Spain","volume":"8","author":"Mattioni","year":"2012","journal-title":"Tree Genet. 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