{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:05:33Z","timestamp":1760058333994,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia, I.P.","award":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"],"award-info":[{"award-number":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"]}]},{"name":"Centre for Functional Ecology\u2014Science for the People &amp; the Planet","award":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"],"award-info":[{"award-number":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"]}]},{"DOI":"10.13039\/501100001871","name":"Ph.D. fellowships financed by FCT\/MCTES through national funds (PIDDAC)","doi-asserted-by":"publisher","award":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"],"award-info":[{"award-number":["UIDB\/04004\/2025","2021.04958.BD","SFRH\/BD\/146485\/2019","SFRH\/BD\/136925\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Solanum\u00a0betaceum Cav. (tamarillo) has a strong biotechnological potential given the ease of obtaining cell lines from it that can be genetically transformed. However, genetic transformation of tamarillo cell suspension cultures has not yet been described. This study presents a simple method for Agrobacterium-mediated transformation of these cells and demonstrates the successful insertion of the \u03b2-glucuronidase gene (gusA) and the yellow fluorescent protein gene (eyfp) in their genome. For the success of this protocol, the selection of actively growing sub-cultured callus as explant and isolation of bacterial colonies with a cell density OD600 of 0.6\u20130.8 were key steps. Also, the inoculation of the callus in a bacteria liquid culture, the use of sonication, and the addition of antioxidants were essential. The transient expression of the gusA gene in tamarillo callus was confirmed and quantified, and no significant differences were observed between using LBA4404 or EHA105 strains. Finally, the insertion of the eyfp gene in the tamarillo genome enabled the in vivo confirmation of the transformation success. The present study showed that tamarillo cell suspension cultures can be genetically modified, opening the way for metabolite production in transformed cells and future scaling-up in bioreactors.<\/jats:p>","DOI":"10.3390\/plants14071028","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T04:17:14Z","timestamp":1742962634000},"page":"1028","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mediated Transformation of Tamarillo (Solanum\u00a0betaceum) Callus Cell Suspension Cultures: A Novel Platform for Biotechnological Applications"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4631-9821","authenticated-orcid":false,"given":"Ricardo","family":"Ferraz","sequence":"first","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"Laqv Requimte, Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1224-3056","authenticated-orcid":false,"given":"Bruno","family":"Casimiro","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"Faculty of Science, Agriculture & Engineering (SAgE), Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0147-0499","authenticated-orcid":false,"given":"Daniela","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"Department of Life Sciences, University of Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2299-298X","authenticated-orcid":false,"given":"Jorge","family":"Canhoto","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2151-3916","authenticated-orcid":false,"given":"Sandra","family":"Correia","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"},{"name":"InnovPlantProtect CoLAb, Estrada de Gil Vaz, 7350-478 Elvas, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Motti, R. 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