{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T15:30:03Z","timestamp":1778340603573,"version":"3.51.4"},"reference-count":119,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009042","name":"VI Plan Propio de Investigaci\u00f3n y Transferencia of the University of Seville II.2A (VIPPIT-US)","doi-asserted-by":"publisher","award":["USE-23098-Y"],"award-info":[{"award-number":["USE-23098-Y"]}],"id":[{"id":"10.13039\/100009042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009042","name":"VI Plan Propio de Investigaci\u00f3n y Transferencia of the University of Seville II.2A (VIPPIT-US)","doi-asserted-by":"publisher","award":["PD21-00003"],"award-info":[{"award-number":["PD21-00003"]}],"id":[{"id":"10.13039\/100009042","id-type":"DOI","asserted-by":"publisher"}]},{"name":"La Caixa Foundation and Foundation for Science and Technology","award":["USE-23098-Y"],"award-info":[{"award-number":["USE-23098-Y"]}]},{"name":"La Caixa Foundation and Foundation for Science and Technology","award":["PD21-00003"],"award-info":[{"award-number":["PD21-00003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Tuberaria lignosa (Sweet) Samp. (Cistaceae) is a herbaceous species native to southwestern Europe, traditionally used to treat wounds, ulcers, and inflammatory or infectious skin conditions. This study aimed to characterize the phytochemical profile of its aqueous leaf extract and evaluate its skin-related in vitro biological activities. The phenolic composition was determined using UHPLC-HRMS\/MS, HPLC-DAD, and quantitative colorimetric assays. Antioxidant activity was assessed against synthetic free radicals, reactive oxygen and nitrogen species, transition metals, and pro-oxidant enzymes. Enzymatic inhibition of tyrosinase, hyaluronidase, collagenase, and elastase were evaluated using in vitro assays. Cytocompatibility was tested on human keratinocytes and NIH\/3T3 fibroblasts using MTT and resazurin assays, respectively, while wound healing was evaluated on NIH\/3T3 fibroblasts using the scratch assay. Antifungal activity was investigated against several Candida and dermatophyte species, while antibiofilm activity was tested against Epidermophyton floccosum. The extract was found to be rich in phenolic compounds, accounting for nearly 45% of its dry weight. These included flavonoids, phenolic acids, and proanthocyanidins, with ellagitannins (punicalagin) being the predominant group. The extract demonstrated potent antioxidant, anti-tyrosinase, anti-collagenase, anti-elastase, and antidermatophytic activities, including fungistatic, fungicidal, and antibiofilm effects. These findings highlight the potential of T. lignosa as a valuable and underexplored source of bioactive phenolic compounds with strong potential for the development of innovative approaches for skin care and therapy.<\/jats:p>","DOI":"10.3390\/plants14152299","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T12:56:43Z","timestamp":1753448203000},"page":"2299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemical Analysis and Therapeutic Potential of Tuberaria lignosa (Sweet) Samp. Aqueous Extract in Skin Injuries"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1458-6554","authenticated-orcid":false,"given":"Manuel","family":"Gonz\u00e1lez-V\u00e1zquez","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}]},{"given":"Ana Qu\u00edlez","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4128-9010","authenticated-orcid":false,"given":"M\u00f3nica","family":"Zuzarte","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research, Centre for Innovative Biomedicine and Biotechnology (iCBR-CIBB), University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Department of Chemical Engineering, Chemical Engineering and Renewable Resources for Sustainability (CERES), University of Coimbra, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0948-821X","authenticated-orcid":false,"given":"L\u00edgia","family":"Salgueiro","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Department of Chemical Engineering, Chemical Engineering and Renewable Resources for Sustainability (CERES), University of Coimbra, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5270-3993","authenticated-orcid":false,"given":"Jorge","family":"Alves-Silva","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research, Centre for Innovative Biomedicine and Biotechnology (iCBR-CIBB), University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2450-1622","authenticated-orcid":false,"given":"Mar\u00eda Luisa","family":"Gonz\u00e1lez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8996-8015","authenticated-orcid":false,"given":"Roc\u00edo De la","family":"Puerta","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","first-page":"475","article-title":"The physiology of wound healing","volume":"29","author":"Young","year":"2011","journal-title":"Surgery"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Cedillo-Cortezano, M., Martinez-Cuevas, L.R., Lopez, J.A.M., Barrera Lopez, I.L., Escutia-Perez, S., and Petricevich, V.L. 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