{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:58:42Z","timestamp":1773104322030,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:00:00Z","timestamp":1772841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mobilizing Agenda TEC4GREEN","award":["C05-i01.01\u20142022.PC644948561-00000052"],"award-info":[{"award-number":["C05-i01.01\u20142022.PC644948561-00000052"]}]},{"name":"iNOVA4Health\u2014Programme in Translational Medicine","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}]},{"name":"R&D Unit GREEN-IT\u2014Bioresources for Sustainability","award":["UID\/04551\/2025"],"award-info":[{"award-number":["UID\/04551\/2025"]}]},{"name":"R&D Unit GREEN-IT\u2014Bioresources for Sustainability","award":["UID\/PRR\/04551\/2025"],"award-info":[{"award-number":["UID\/PRR\/04551\/2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Pear scab, caused by Venturia pyrina, poses a threat to pear cultivation, with particularly severe consequences for Portugal\u2019s high-value Rocha pear industry. Despite its economic impact, few biological control agents are currently available. In this work, the phenotypic and genomic characterization of Pseudomonas capeferrum NFX1 is performed and its role as an effective biocontrol agent against V. pyrina is reported. Detailed genomic analysis revealed that strain NFX1 and other members of the Pseudomonas capeferrum species contain key biosynthetic gene clusters involved in pathogen antagonism, including the cyclic lipopeptide putisolvin. Phenotypic assays showed that strain NFX1 significantly inhibited V. pyrina growth, spore germination, and reduced pear scab lesion severity and fungal colonization in detached leaf assays. Moreover, strain NFX1 reprogrammed the Rocha pear leaf transcriptome to be consistent with a priming state and induced systemic resistance. A novel image-based method quantifying lesion darkening as a proxy for pear scab severity in detached leaves and a qPCR assay targeting the V. pyrina ef1-\u03b1 gene and optimized for fungal DNA detection in infected pear leaves were also developed, thereby establishing a laboratory workflow specifically tailored to biocontrol evaluation against V. pyrina. Ultimately, the obtained results demonstrated the potential of P. capeferrum NFX1 for sustainable pear scab control.<\/jats:p>","DOI":"10.3390\/plants15050823","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T13:27:18Z","timestamp":1773062838000},"page":"823","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pear Scab Disease Suppression by Pseudomonas capeferrum NFX1 Is Mediated by Direct Antagonism Against Venturia pyrina and Pear Defense Priming"],"prefix":"10.3390","volume":"15","author":[{"given":"Sara","family":"Tedesco","sequence":"first","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"given":"Margarida","family":"Pimenta","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"given":"Filipa T.","family":"Silva","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0610-7664","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Baixinho","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-6230","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Bustos Gaspar","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5605-1419","authenticated-orcid":false,"given":"Maria Teresa","family":"Barreto Crespo","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1184-3794","authenticated-orcid":false,"given":"Francisco X.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fonseca, A.M.A., Dias, C., Amaro, A.L., Isidoro, N., Pintado, M., Silvestre, A.J.D., and Rocha, S.M. (2020). The Impact of Plant-Based Coatings in \u201cROCHA\u201d Pear Preservation during Cold Storage: A Metabolomic Approach. Foods, 9.","DOI":"10.3390\/foods9091299"},{"key":"ref_2","unstructured":"(2025, September 26). Rocha Pear Website. Available online: https:\/\/perarocha.pt\/."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Queiroz, \u00c1., Bagoin Guimar\u00e3es, J., S\u00e1nchez, C., Sim\u00f5es, F., Maia de Sousa, R., Viegas, W., and Veloso, M.M. (2019). Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm. Sustainability, 11.","DOI":"10.3390\/su11195340"},{"key":"ref_4","first-page":"26","article-title":"Review of the pear scab caused by Venturia Pyrina","volume":"1","author":"Sokolova","year":"2014","journal-title":"Res. Rural. Dev."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Silva, J.M., Barba, N.G., Barros, M.T., and Torres-Paulo, A. (2005). \u2018Rocha\u2019, the Pear from Portugal. Acta Hortic., 219\u2013222.","DOI":"10.17660\/ActaHortic.2005.671.29"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.scienta.2008.11.005","article-title":"Segregation and transmission of host resistance to scab (Venturia pirina) in pear breeding progeny under natural infection in an orchard","volume":"120","author":"Liu","year":"2009","journal-title":"Sci. Hortic."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/jph.13002","article-title":"Evaluation of Venturia pyrina virulence on European pear (Pyrus communis) cultivars by an in vitro methodology","volume":"169","author":"Sokolova","year":"2021","journal-title":"J. Phytopathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1094\/PDIS.2000.84.6.681","article-title":"Pear Scab: Components of Potential Ascospore Dose and Validation of an Ascospore Maturity Model","volume":"84","author":"Spotts","year":"2000","journal-title":"Plant Dis"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Dom\u00ednguez, E., Armengol, J., and Rossi, V. (2017). Biology and Epidemiology of Venturia Species Affecting Fruit Crops: A Review. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01496"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1002\/aepp.13239","article-title":"The EU\u2019s farm-to-fork strategy: An assessment from the perspective of agricultural economics","volume":"44","author":"Wesseler","year":"2022","journal-title":"Appl. Econ. Perspect. Policy"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"K\u00f6hl, J., Kolnaar, R., and Ravensberg, W.J. (2019). Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00845"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/978-1-0716-2517-0_26","article-title":"A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents","volume":"2536","author":"Macan","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e2810","DOI":"10.21769\/BioProtoc.2810","article-title":"In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato","volume":"8","author":"Lian","year":"2018","journal-title":"Bio. Protoc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.phyto.41.052002.095656","article-title":"Regulation of Antibiotic Production in Root-Colonizing Pseudomonas spp. and Relevance for Biological Control of Plant Disease","volume":"41","author":"Haas","year":"2003","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_15","unstructured":"Sariaslani, S., and Gadd, G.M. (2022). The use of Pseudomonas spp. as bacterial biocontrol agents to control plant diseases. Achievements in the Life Sciences, Springer."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Alattas, H., Glick, B.R., Murphy, D.V., and Scott, C. (2024). Harnessing Pseudomonas spp. for sustainable plant crop protection. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1485197"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e000053","DOI":"10.1099\/acmi.0.000053","article-title":"Isolation and characterization of novel soil- and plant-associated bacteria with multiple phytohormone-degrading activities using a targeted methodology","volume":"1","author":"Nascimento","year":"2019","journal-title":"Access Microbiol."},{"key":"ref_18","unstructured":"Stringer, P. (2026, February 22). Pear IPM: Pear Scab. University of Massachusetts Amherst Extension. Available online: https:\/\/www.umass.edu\/agriculture-food-environment\/fruit\/fact-sheets\/pear-ipm-pear-scab."},{"key":"ref_19","unstructured":"Spotts, R.A., and Castagnoli, S. (2026, February 22). Pear Scab in Oregon: Symptoms, Disease Cycle and Management. Oregon State University Extension Service. Available online: https:\/\/extension.oregonstate.edu\/sites\/extd8\/files\/documents\/em9003.pdf."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6371","DOI":"10.1128\/AEM.68.12.6371-6382.2002","article-title":"Identification Characterization of an N-Acylhomoserine Lactone-Dependent Quorum-Sensing System in Pseudomonas putida Strain IsoF","volume":"68","author":"Steidle","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1038\/s41564-022-01209-6","article-title":"Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system","volume":"7","author":"Agnoli","year":"2022","journal-title":"Nat. Microbiol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1128\/aem.58.9.2978-2982.1992","article-title":"Effect of pseudobactin 358 production by Pseudomonas putida WCS358 on suppression of fusarium wilt of carnations by nonpathogenic Fusarium oxysporum Fo47","volume":"58","author":"Lemanceau","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1080\/09583159409355336","article-title":"Suppression of fusarium wilt of carnation by Pseudomonas putida WCS358 at different levels of disease incidence and iron availability","volume":"4","author":"Duijff","year":"1994","journal-title":"Biocontrol. Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zboralski, A., Biessy, A., Ciotola, M., Cadieux, M., Albert, D., Blom, J., and Filion, M. (2022). Harnessing the genomic diversity of Pseudomonas strains against lettuce bacterial pathogens. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1038888"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Mata, A.S., Cruz, C., Gaspar, J.R., Abrantes, I., Concei\u00e7\u00e3o, I.L., Morais, P.V., and Proen\u00e7a, D.N. (2025). Plant growth-promoting bacteria as biological control agents for sustainable agriculture: Targeting root-knot nematodes. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1567265"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.1099\/mic.0.2008\/016444-0","article-title":"Genetic and functional characterization of the gene cluster directing the biosynthesis of putisolvin I and II in Pseudomonas putida strain PCL1445","volume":"154","author":"Dubern","year":"2008","journal-title":"Microbiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1046\/j.1365-2958.2003.03751.x","article-title":"Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms","volume":"51","author":"Kuiper","year":"2004","journal-title":"Mol. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/j.1365-2672.2009.04244.x","article-title":"Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promoting Pseudomonas putida 267","volume":"107","author":"Kruijt","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s41348-024-00874-5","article-title":"The biocontrol roles of cyclic lipopeptide putisolvin produced from Pseudomonas capeferrum HN2-3 on the Phytophthora blight disease in cucumbers","volume":"131","author":"Sheng","year":"2024","journal-title":"J. Plant Dis. Prot."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1128\/JB.188.8.2898-2906.2006","article-title":"The ppuI-rsaL-ppuR Quorum-Sensing System Regulates Biofilm Formation of Pseudomonas putida PCL1445 by Controlling Biosynthesis of the Cyclic Lipopeptides Putisolvins I and II","volume":"188","author":"Dubern","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1094\/MPMI.1997.10.6.716","article-title":"Differential Induction of Systemic Resistance in Arabidopsis by Biocontrol Bacteria","volume":"10","author":"Pieterse","year":"1997","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s10658-005-0623-3","article-title":"Induction of Systemic Resistance Against Bacterial Wilt in Eucalyptus urophylla by Fluorescent Pseudomonas spp.","volume":"113","author":"Ran","year":"2005","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1364-3703.2005.00276.x","article-title":"Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants","volume":"6","author":"Meziane","year":"2005","journal-title":"Mol. Plant Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1574-6941.2008.00436.x","article-title":"Discovery of a bacterial gene cluster for catabolism of the plant hormone indole 3-acetic acid","volume":"65","author":"Leveau","year":"2008","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.21105\/joss.03941","article-title":"Rasusa: Randomly subsample sequencing reads to a specified coverage","volume":"7","author":"Hall","year":"2022","journal-title":"J. Open Source Softw."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e00242-18","DOI":"10.1128\/genomeA.00242-18","article-title":"Near-Complete Genome Sequence of Pseudomonas palleroniana MAB3, a Beneficial 1-Aminocyclopropane-1-Carboxylate Deaminase-Producing Bacterium Able to Promote the Growth of Mushrooms and Plants","volume":"6","author":"Giachini","year":"2018","journal-title":"Genome Announc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Walker, B.J., Abeel, T., Shea, T., Priest, M., Abouelliel, A., Sakthikumar, S., Cuomo, C.A., Zeng, Q., Wortman, J., and Young, S.K. (2014). Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112963"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-depth characterization and visualization of bacterial genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"antiSMASH 7.0: New and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1099\/ijsem.0.000760","article-title":"OrthoANI: An improved algorithm and software for calculating average nucleotide identity","volume":"66","author":"Lee","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1534\/g3.119.400047","article-title":"Population Genome Sequencing of the Scab Fungal Species Venturia inaequalis, Venturia pirina, Venturia aucupariae and Venturia asperata","volume":"9","author":"Sargent","year":"2019","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Assun\u00e7\u00e3o, M., Santos, C., Braz\u00e3o, J., Eiras-Dias, J.E., and Fevereiro, P. (2019). Understanding the molecular mechanisms underlying graft success in grapevine. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1967-8"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2014New capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3420","DOI":"10.1093\/nar\/gkn176","article-title":"High-throughput functional annotation and data mining with the Blast2GO suite","volume":"36","author":"Gotz","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wickham, H., Chang, W., Henry, L., Pedersen, T.L., Takahashi, K., Wilke, C., Woo, K., Yutani, H., Dunnington, D., and van den Brand, T. (2007). ggplot2: Create Elegant Data Visualisations Using the Grammar of Graphics, The Comprehensive R Archive Network (CRAN). Preprint at the comprehensive R Archive Network (CRAN).","DOI":"10.32614\/CRAN.package.ggplot2"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Campitelli, E. (2019). ggnewscale: Multiple Fill and Colour Scales in \u2018ggplot2\u2019, The Comprehensive R Archive Network (CRAN). Preprint at the comprehensive R Archive Network (CRAN).","DOI":"10.32614\/CRAN.package.ggnewscale"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","article-title":"Complex heatmaps reveal patterns and correlations in multidimensional genomic data","volume":"32","author":"Gu","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_52","unstructured":"de Mendiburu, F. (2026, March 03). R package, Available online: https:\/\/cran.r-project.org\/web\/packages\/agricolae\/index.html."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/5\/823\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T13:51:59Z","timestamp":1773064319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/5\/823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,7]]},"references-count":52,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["plants15050823"],"URL":"https:\/\/doi.org\/10.3390\/plants15050823","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,7]]}}}