{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T03:52:19Z","timestamp":1773546739137,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:00:00Z","timestamp":1583971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","award":["0302_CVMAR_I_1_P"],"award-info":[{"award-number":["0302_CVMAR_I_1_P"]}],"id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Understanding the conditions affecting cyanobacterial biofilm development is crucial to develop new antibiofouling strategies and decrease the economic and environmental impact of biofilms in marine settings. In this study, we investigated the relative importance of shear forces and surface hydrophobicity on biofilm development by two coccoid cyanobacteria with different biofilm formation capacities. The strong biofilm-forming Synechocystis salina was used along with the weaker biofilm-forming Cyanobium sp. Biofilms were developed in defined hydrodynamic conditions using glass (a model hydrophilic surface) and a polymeric epoxy coating (a hydrophobic surface) as substrates. Biofilms developed in both surfaces at lower shear conditions contained a higher number of cells and presented higher values for wet weight, thickness, and chlorophyll a content. The impact of hydrodynamics on biofilm development was generally stronger than the impact of surface hydrophobicity, but a combined effect of these two parameters strongly affected biofilm formation for the weaker biofilm-producing organism. The antibiofilm performance of the polymeric coating was confirmed at the hydrodynamic conditions prevailing in ports. Shear forces were shown to have a profound impact on biofilm development in marine settings regardless of the fouling capacity of the existing flora and the hydrophobicity of the surface.<\/jats:p>","DOI":"10.3390\/polym12030653","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T08:58:59Z","timestamp":1584089939000},"page":"653","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Relative Importance of Shear Forces and Surface Hydrophobicity on Biofilm Formation by Coccoid Cyanobacteria"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4819-1892","authenticated-orcid":false,"given":"Sara I.","family":"Faria","sequence":"first","affiliation":[{"name":"LEPABE\u2014Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-9362","authenticated-orcid":false,"given":"Rita","family":"Teixeira-Santos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1094-5425","authenticated-orcid":false,"given":"Maria J.","family":"Romeu","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Morais","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"FCUP\u2014Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4069-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe J.","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1002\/adma.201001215","article-title":"Antifouling coatings: Recent developments in the design of surfaces that prevent fouling by proteins, bacteria, and marine organisms","volume":"23","author":"Banerjee","year":"2011","journal-title":"Adv. Mater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6547","DOI":"10.1039\/C5TB00232J","article-title":"Non-toxic, non-biocide-release antifouling coatings based on molecular structure design for marine applications","volume":"3","author":"Nurioglu","year":"2015","journal-title":"J. Mater. Chem. B"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1016\/j.scitotenv.2018.10.010","article-title":"Eco-friendly non-biocide-release coatings for marine biofouling prevention","volume":"650","author":"Silva","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1080\/08927014.2010.542809","article-title":"Economic impact of biofouling on a naval surface ship","volume":"27","author":"Schultz","year":"2011","journal-title":"Biofouling"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1007\/s10530-012-0230-0","article-title":"Modeling biofouling from boat and source characteristics: A comparative study between Canada and New Zealand","volume":"14","author":"Forrest","year":"2012","journal-title":"Biol. Invasions"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1300\/J028v18n01_05","article-title":"Comparison of bacterial presence in biofilms on different materials commonly found in recirculating aquaculture systems","volume":"18","author":"King","year":"2006","journal-title":"J. Appl. Aquacult."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Telegdi, J., Trif, L., and Rom\u00e1nszki, L. (2016). Smart Anti-Biofouling Composite Coatings for Naval Applications, in Smart Composite Coatings and Membranes, Elsevier.","DOI":"10.1016\/B978-1-78242-283-9.00005-1"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1016\/j.marpolbul.2011.02.031","article-title":"Antifouling activity of commercial biocides vs. natural and natural-derived products assessed by marine bacteria adhesion bioassay","volume":"62","author":"Camps","year":"2011","journal-title":"Mar. Pollut. Bull."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3389\/fmars.2018.00126","article-title":"Marine biofilms: A successful microbial strategy with economic implications","volume":"5","author":"Carvalho","year":"2018","journal-title":"Front. Mar. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jembe.2016.03.009","article-title":"Enzymes manage biofilms on crab surfaces aiding in feeding and antifouling","volume":"479","author":"Wepprich","year":"2016","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1016\/j.surfcoat.2006.08.129","article-title":"Modern approaches to marine antifouling coatings","volume":"201","author":"Chambers","year":"2006","journal-title":"Surf. Coat. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s10295-007-0234-4","article-title":"Bacterial cell attachment, the beginning of a biofilm","volume":"34","author":"Palmer","year":"2007","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1007\/s11434-010-4158-4","article-title":"Progress of marine biofouling and antifouling technologies","volume":"56","author":"Cao","year":"2011","journal-title":"Chin. Sci. Bull."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4031\/MTSJ.51.2.5","article-title":"Evidence-based decision making to underpin the thresholds in New Zealand\u2019s craft risk management standard: Biofouling on vessels arriving to New Zealand","volume":"51","author":"Georgiades","year":"2017","journal-title":"Mar. Technol. Soc. J."},{"key":"ref_15","unstructured":"McClay, T., Zabin, C., Davidson, I., Young, R., and Elam, D. (2015). Vessel Biofouling Prevention and Management Options Report."},{"key":"ref_16","unstructured":"Takata, L., Falkner, M., and Gilmore, S. (2006). Analysis, Evaluation, and Recommendations to Reduce Nonindigenous Species Release from the Non-Ballast Water Vector."},{"key":"ref_17","unstructured":"Ministry of Primary Industries (2018). Craft Risk Management Standard: Biofouling on Vessels Arriving to New Zealand."},{"key":"ref_18","unstructured":"Ministry of Primary Industries (2018). The Craft Risk Management Standard for Biofouling: Frquently Asked Questions."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: Microbial life on surfaces","volume":"8","author":"Donlan","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4411","DOI":"10.1111\/1462-2920.14807","article-title":"Biofilm formation behaviour of marine filamentous cyanobacterial strains in controlled hydrodynamic conditions","volume":"21","author":"Romeu","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Taylor, D.A. (1996). Introduction to Marine Engineering, Elsevier.","DOI":"10.1016\/B978-075062530-2\/50017-9"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Patel, A., Kravchenko, O., and Manas-Zloczower, I. (2018). Effect of curing rate on the microstructure and macroscopic properties of epoxy fiberglass composites. Polymers, 10.","DOI":"10.3390\/polym10020125"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.repl.2016.01.002","article-title":"Epoxy resin infused boat hulls","volume":"60","author":"Hoge","year":"2016","journal-title":"Reinf. Plast."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1039\/B314073C","article-title":"High frequency monitoring of the coastal marine environment using the MAREL buoy","volume":"6","author":"Blain","year":"2004","journal-title":"J. Environ. Monit."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"503","DOI":"10.5194\/os-6-503-2010","article-title":"Biofouling protection for marine environmental sensors","volume":"6","author":"Delauney","year":"2010","journal-title":"Ocean Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ijfoodmicro.2018.04.017","article-title":"Impact of modified diamond-like carbon coatings on the spatial organization and disinfection of mixed-biofilms composed of Escherichia coli and Pantoea agglomerans industrial isolates","volume":"277","author":"Gomes","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.lwt.2017.01.038","article-title":"Characterization of the heterotrophic bacteria from a minimally processed vegetables plant","volume":"85","author":"Meireles","year":"2017","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1111\/j.1365-2672.2006.03029.x","article-title":"Adhesion of water stressed Helicobacter pylori to abiotic surfaces","volume":"101","author":"Azevedo","year":"2006","journal-title":"J. Appl. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1002\/jbm.a.35277","article-title":"Escherichia coli adhesion, biofilm development and antibiotic susceptibility on biomedical materials","volume":"103","author":"Gomes","year":"2015","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1007\/s10811-017-1369-y","article-title":"Cyanobacterial diversity held in microbial biological resource centers as a biotechnological asset: The case study of the newly established LEGE culture collection","volume":"30","author":"Ramos","year":"2018","journal-title":"J. Appl. Phycol."},{"key":"ref_31","unstructured":"Kotai, J. (1972). Instructions for Preparation of Modified Nutrient Solution Z8 for AlgaeNorwegian, Institute for Water Research."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6280","DOI":"10.1128\/AEM.69.10.6280-6287.2003","article-title":"Comparison of velocity profiles for different flow chamber designs used in studies of microbial adhesion to surfaces","volume":"69","author":"Bakker","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Flemming, H.C., Murthy, P.S., Venkatesan, R., and Cooksey, K. (2009). Marine and Industrial Biofouling, Springer.","DOI":"10.1007\/978-3-540-69796-1"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1080\/0892701021000057891","article-title":"Fouling and ships\u2019 hulls: H changing circumstances and spawning events may result in the spread of exotic species","volume":"19","author":"Minchin","year":"2003","journal-title":"Biofouling"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"S56","DOI":"10.1016\/j.ecolind.2008.11.013","article-title":"Phytoplankton bloom status: Chlorophyll a biomass as an indicator of water quality condition in the southern estuaries of Florida, USA","volume":"9","author":"Boyer","year":"2009","journal-title":"Ecol. Indic."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/S0005-2728(89)80347-0","article-title":"Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy","volume":"975","author":"Porra","year":"1989","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_37","first-page":"300","article-title":"Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic substrata","volume":"26","author":"Bayoudh","year":"2006","journal-title":"Mater. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7455","DOI":"10.1016\/j.polymer.2007.10.038","article-title":"Fabrication of super-hydrophobic film from PMMA with intrinsic water contact angle below 90","volume":"48","author":"Ma","year":"2007","journal-title":"Polymer"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Bartram, J., and Chorus, I. (1999). Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring and Management, CRC Press.","DOI":"10.1201\/9781482295061"},{"key":"ref_40","unstructured":"Rees, G., and Bartram, J. (2002). Monitoring Bathing Waters: A Practical Guide to the Design and Implementation of Assessments and Monitoring Programmes, CRC Press."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.resmic.2005.01.007","article-title":"Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis","volume":"156","author":"Cerca","year":"2005","journal-title":"Res. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1080\/08927010903531491","article-title":"Role of hydrophobicity in bacterial adherence to carbon nanostructures and biofilm formation","volume":"26","author":"Mazumder","year":"2010","journal-title":"Biofouling"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Thomen, P., Robert, J., Monmeyran, A., Bitbol, A.F., Douarche, C., and Henry, N. (2017). Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0175197"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nmicrobiol.2015.5","article-title":"Local and global consequences of flow on bacterial quorum sensing","volume":"1","author":"Kim","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"8357","DOI":"10.1128\/JB.01040-07","article-title":"Influence of the hydrodynamic environment on quorum sensing in Pseudomonas aeruginosa biofilms","volume":"189","author":"Kirisits","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1046\/j.1365-2672.2001.01244.x","article-title":"Metabolic response of biofilm to shear stress in fixed-film culture","volume":"90","author":"Liu","year":"2001","journal-title":"J. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4345","DOI":"10.1073\/pnas.1300321110","article-title":"Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems","volume":"110","author":"Drescher","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/3\/653\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:06:28Z","timestamp":1760173588000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/3\/653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,12]]},"references-count":47,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["polym12030653"],"URL":"https:\/\/doi.org\/10.3390\/polym12030653","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,12]]}}}