{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:17:16Z","timestamp":1770347836612,"version":"3.49.0"},"reference-count":77,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>The possibility of utilizing lignocellulosic agro-industrial waste products such as cassava peel hydrolysate (CPH) as carbon sources for polyhydroxybutyrate (PHB) biosynthesis and characterization by Amazonian microalga Stigeoclonium sp. B23. was investigated. Cassava peel was hydrolyzed to reducing sugars to obtain increased glucose content with 2.56 \u00b1 0.07 mmol\/L. Prior to obtaining PHB, Stigeoclonium sp. B23 was grown in BG-11 for characterization and Z8 media for evaluation of PHB nanoparticles\u2019 cytotoxicity in zebrafish embryos. As results, microalga produced the highest amount of dry weight of PHB with 12.16 \u00b1 1.28 (%) in modified Z8 medium, and PHB nanoparticles exerted some toxicity on zebrafish embryos at concentrations of 6.25\u2013100 \u00b5g\/mL, increased mortality (&lt;35%) and lethality indicators as lack of somite formation (&lt;25%), non-detachment of tail, and lack of heartbeat (both &lt;15%). Characterization of PHB by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimeter (DSC), and thermogravimetry (TGA) analysis revealed the polymer obtained from CPH cultivation to be morphologically, thermally, physically, and biologically acceptable and promising for its use as a biomaterial and confirmed the structure of the polymer as PHB. The findings revealed that microalgal PHB from Stigeoclonium sp. B23 was a promising and biologically feasible new option with high commercial value, potential for biomaterial applications, and also suggested the use of cassava peel as an alternative renewable resource of carbon for PHB biosynthesis and the non-use of agro-industrial waste and dumping concerns.<\/jats:p>","DOI":"10.3390\/polym13050687","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T04:36:24Z","timestamp":1614314184000},"page":"687","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization and Biotechnological Potential of Intracellular Polyhydroxybutyrate by Stigeoclonium sp. B23 Using Cassava Peel as Carbon Source"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2038-3099","authenticated-orcid":false,"given":"Murilo Moraes","family":"Mour\u00e3o","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Par\u00e1, 66075-110 Bel\u00e9m, Par\u00e1, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0913-8765","authenticated-orcid":false,"given":"Luciana Pereira","family":"Xavier","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Par\u00e1, 66075-110 Bel\u00e9m, Par\u00e1, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7476-9195","authenticated-orcid":false,"given":"Ralph","family":"Urbatzka","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research\u2014CIIMAR, University of Porto, 4450-208 Porto, Portugal"}]},{"given":"Lucas Barbosa","family":"Figueiroa","sequence":"additional","affiliation":[{"name":"Laboratory of Oils of the Amazon, Guam\u00e1 Science and Technology Park, Federal University of Par\u00e1, 66075-750 Bel\u00e9m, Par\u00e1, Brazil"}]},{"given":"Carlos Emmerson Ferreira da","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Oils of the Amazon, Guam\u00e1 Science and Technology Park, Federal University of Par\u00e1, 66075-750 Bel\u00e9m, Par\u00e1, Brazil"}]},{"given":"Carmen Gilda Barroso Tavares","family":"Dias","sequence":"additional","affiliation":[{"name":"Laboratory of Materials Processing, Institute of Technology, Federal University of Par\u00e1, 66075-110 Bel\u00e9m, Par\u00e1, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2538-4827","authenticated-orcid":false,"given":"Maria Paula Cruz","family":"Schneider","sequence":"additional","affiliation":[{"name":"Genomics and Systems Biology Center, Federal University of Par\u00e1, 66075-110 Bel\u00e9m, Par\u00e1, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research\u2014CIIMAR, University of Porto, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4069-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2690-2841","authenticated-orcid":false,"given":"Agenor Valadares","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Par\u00e1, 66075-110 Bel\u00e9m, Par\u00e1, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1021\/cr200162d","article-title":"Plastics derived from biological sources: Present and future: A technical and environmental review","volume":"112","author":"Chen","year":"2011","journal-title":"Chem. 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