{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:55:05Z","timestamp":1768640105391,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020; UIDB\/04378\/2020; LA\/P\/0140\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020; UIDB\/04378\/2020; LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>In this study, membrane-based methods were evaluated for the recovery of FucoPol, the fucose-rich exopolysaccharide (EPS) secreted by the bacterium Enterobacter A47, aiming at reducing the total water consumption and extraction time, while keeping a high product recovery, thus making the downstream procedure more sustainable and cost-effective. The optimized method involved ultrafiltration of the cell-free supernatant using a 30 kDa molecular weight cut-off (MWCO) membrane that allowed for a 37% reduction of the total water consumption and a 55% reduction of the extraction time, compared to the previously used method (diafiltration-ultrafiltration with a 100 kDa MWCO membrane). This change in the downstream procedure improved the product\u2019s recovery (around 10% increase) and its purity, evidenced by the lower protein (8.2 wt%) and inorganic salts (4.0 wt%) contents of the samples (compared to 9.3 and 8.6 wt%, respectively, for the previously used method), without impacting FucoPol\u2019s sugar and acyl groups composition, molecular mass distribution or thermal degradation profile. The biopolymer\u2019s emulsion-forming and stabilizing capacity was also not affected (emulsification activity (EA) with olive oil, at a 2:3 ratio, of 98 \u00b1 0% for all samples), while the rheological properties were improved (the zero-shear viscosity increased from 8.89 \u00b1 0.62 Pa\u00b7s to 17.40 \u00b1 0.04 Pa\u00b7s), which can be assigned to the higher purity degree of the extracted samples. These findings demonstrate a significant improvement in the downstream procedure raising FucoPol\u2019s recovery, while reducing water consumption and operation time, key criteria in terms of process economic and environmental sustainability. Moreover, those changes improved the biopolymer\u2019s rheological properties, known to significantly impact FucoPol\u2019s utilization in cosmetic, pharmaceutical or food products.<\/jats:p>","DOI":"10.3390\/polym14030390","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T21:01:51Z","timestamp":1642626111000},"page":"390","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties"],"prefix":"10.3390","volume":"14","author":[{"given":"S\u00edlvia","family":"Baptista","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"73100 Lda., Edif\u00edcio Arcis, Rua Ivone Silva, 6, 4\u00b0 piso, 1050-124 Lisboa, Portugal"}]},{"given":"Cristiana A. V.","family":"Torres","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"}]},{"given":"Chantal","family":"Sevrin","sequence":"additional","affiliation":[{"name":"Interfaculty Research Centre of Biomaterials (CEIB), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}]},{"given":"Christian","family":"Grandfils","sequence":"additional","affiliation":[{"name":"Interfaculty Research Centre of Biomaterials (CEIB), University of Li\u00e8ge, B-4000 Li\u00e8ge, Belgium"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-1836","authenticated-orcid":false,"given":"Maria A. 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