{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:23:33Z","timestamp":1777069413621,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T00:00:00Z","timestamp":1654992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EUROPEAN UNION\u2019S H2020 research and innovation program","award":["861138"],"award-info":[{"award-number":["861138"]}]},{"name":"EUROPEAN UNION\u2019S H2020 research and innovation program","award":["KP-06-H48\/6"],"award-info":[{"award-number":["KP-06-H48\/6"]}]},{"name":"EUROPEAN UNION\u2019S H2020 research and innovation program","award":["899673"],"award-info":[{"award-number":["899673"]}]},{"name":"BULGARIAN NATIONAL SCIENCE FUND (NSF)","award":["861138"],"award-info":[{"award-number":["861138"]}]},{"name":"BULGARIAN NATIONAL SCIENCE FUND (NSF)","award":["KP-06-H48\/6"],"award-info":[{"award-number":["KP-06-H48\/6"]}]},{"name":"BULGARIAN NATIONAL SCIENCE FUND (NSF)","award":["899673"],"award-info":[{"award-number":["899673"]}]},{"name":"H2020 FET Open METAFAST","award":["861138"],"award-info":[{"award-number":["861138"]}]},{"name":"H2020 FET Open METAFAST","award":["KP-06-H48\/6"],"award-info":[{"award-number":["KP-06-H48\/6"]}]},{"name":"H2020 FET Open METAFAST","award":["899673"],"award-info":[{"award-number":["899673"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Developing antimicrobial surfaces that combat implant-associated infections while promoting host cell response is a key strategy for improving current therapies for orthopaedic injuries. In this paper, we present the application of ultra-short laser irradiation for patterning the surface of a 3D biodegradable synthetic polymer in order to affect the adhesion and proliferation of bone cells and reject bacterial cells. The surfaces of 3D-printed polycaprolactone (PCL) scaffolds were processed with a femtosecond laser (\u03bb = 800 nm; \u03c4 = 130 fs) for the production of patterns resembling microchannels or microprotrusions. MG63 osteoblastic cells, as well as S. aureus and E. coli, were cultured on fs-laser-treated samples. Their attachment, proliferation, and metabolic activity were monitored via colorimetric assays and scanning electron microscopy. The microchannels improved the wettability, stimulating the attachment, spreading, and proliferation of osteoblastic cells. The same topography induced cell-pattern orientation and promoted the expression of alkaline phosphatase in cells growing in an osteogenic medium. The microchannels exerted an inhibitory effect on S. aureus as after 48 h cells appeared shrunk and disrupted. In comparison, E. coli formed an abundant biofilm over both the laser-treated and control samples; however, the film was dense and adhesive on the control PCL but unattached over the microchannels.<\/jats:p>","DOI":"10.3390\/polym14122382","type":"journal-article","created":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T23:55:24Z","timestamp":1655078124000},"page":"2382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Investigating Potential Effects of Ultra-Short Laser-Textured Porous Poly-\u03b5-Caprolactone Scaffolds on Bacterial Adhesion and Bone Cell Metabolism"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8373-6034","authenticated-orcid":false,"given":"Emil","family":"Filipov","sequence":"first","affiliation":[{"name":"Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, Bulgaria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1702-862X","authenticated-orcid":false,"given":"Liliya","family":"Angelova","sequence":"additional","affiliation":[{"name":"Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, Bulgaria"}]},{"given":"Sanjana","family":"Vig","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Dentaria, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, University of Porto, 4160-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-9574","authenticated-orcid":false,"given":"Maria Helena","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Dentaria, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal"},{"name":"LAQV\/REQUIMTE, University of Porto, 4160-007 Porto, Portugal"}]},{"given":"Gerard","family":"Moreau","sequence":"additional","affiliation":[{"name":"Laboratoire des Mat\u00e9riaux C\u00e9ramiques et Proc\u00e9d\u00e9s Associ\u00e9s, Universit\u00e9 Polytechnique Hauts-de-France, INSA Hauts-de-France, CERAMATHS, F-59313 Valenciennes, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8383-5585","authenticated-orcid":false,"given":"Marie","family":"Lasgorceix","sequence":"additional","affiliation":[{"name":"Laboratoire des Mat\u00e9riaux C\u00e9ramiques et Proc\u00e9d\u00e9s Associ\u00e9s, Universit\u00e9 Polytechnique Hauts-de-France, INSA Hauts-de-France, CERAMATHS, F-59313 Valenciennes, France"}]},{"given":"Ivan","family":"Buchvarov","sequence":"additional","affiliation":[{"name":"Faculty of Physics, St. Kliment Ohridski University of Sofia, 5 James Bourchier Blvd., 1164 Sofia, Bulgaria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1131-6900","authenticated-orcid":false,"given":"Albena","family":"Daskalova","sequence":"additional","affiliation":[{"name":"Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, Bulgaria"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,12]]},"reference":[{"key":"ref_1","first-page":"111","article-title":"Porous Metal for Orthopedics Implants","volume":"10","author":"Matassi","year":"2013","journal-title":"Clin. 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