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cellulose (DCMC) was synthesized and tested for protein adsorption. The prepared polymer was characterized by inductively coupled plasma atomic emission spectrometry (ICP-AES), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and solid state nuclear magnetic resonance (ssNMR) to confirm the functionalization of cellulose. This work shows that protein adsorption onto DCMC is charge dependent. The polymer adsorbs positively charged proteins, cytochrome C and lysozyme, with adsorption capacities of 851 and 571 mg g\u22121, respectively. In both experiments, the adsorption process follows the Langmuir adsorption isotherm. The adsorption kinetics by DCMC is well described by the pseudo second-order model, and adsorption equilibrium was reached within 90 min. Moreover, DCMC was successfully reused for five consecutive adsorption\u2013desorption cycles, without compromising the removal efficiency (98\u201399%).<\/jats:p>","DOI":"10.3390\/polym14235122","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T03:00:13Z","timestamp":1669345213000},"page":"5122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Protein Adsorption Performance of a Novel Functionalized Cellulose-Based Polymer"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7903-4734","authenticated-orcid":false,"given":"Diana","family":"Gago","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0890-6133","authenticated-orcid":false,"given":"Marta C.","family":"Corvo","sequence":"additional","affiliation":[{"name":"i3N\/Cenimat, Materials Science Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4337-5956","authenticated-orcid":false,"given":"Ricardo","family":"Chagas","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"Food4Sustainability\u2014Associa\u00e7\u00e3o para a Inova\u00e7\u00e3o no Alimento Sustent\u00e1vel, Centro Empresarial de Idanha-a-Nova, Zona Industrial, 6060-182 Idanha-a-Nova, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8474-3925","authenticated-orcid":false,"given":"Lu\u00edsa M.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7032-3436","authenticated-orcid":false,"given":"Isabel","family":"Coelhoso","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4099","DOI":"10.1021\/acs.iecr.8b05967","article-title":"Diethylaminoethyl-Modified Magnetic Starlike Organic Spherical Adsorbent: Fabrication, Characterization, and Potential for Protein Adsorption","volume":"58","author":"Qiao","year":"2019","journal-title":"Ind. 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