{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:43:20Z","timestamp":1777517000864,"version":"3.51.4"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"national funds","award":["UIDB\/50011\/2020"],"award-info":[{"award-number":["UIDB\/50011\/2020"]}]},{"name":"national funds","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}]},{"name":"national funds","award":["LA\/P\/0006\/2020"],"award-info":[{"award-number":["LA\/P\/0006\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>This study investigates the osteogenic differentiation of umbilical-cord-derived human mesenchymal stromal cells (hUC-MSCs) on biphasic calcium phosphate (BCP) scaffolds derived from cuttlefish bone doped with metal ions and coated with polymers. First, the in vitro cytocompatibility of the undoped and ion-doped (Sr2+, Mg2+ and\/or Zn2+) BCP scaffolds was evaluated for 72 h using Live\/Dead staining and viability assays. From these tests, the most promising composition was found to be the BCP scaffold doped with strontium (Sr2+), magnesium (Mg2+) and zinc (Zn2+) (BCP-6Sr2Mg2Zn). Then, samples from the BCP-6Sr2Mg2Zn were coated with poly(\u0511-caprolactone) (PCL) or poly(ester urea) (PEU). The results showed that hUC-MSCs can differentiate into osteoblasts, and hUC-MSCs seeded on the PEU-coated scaffolds proliferated well, adhered to the scaffold surfaces, and enhanced their differentiation capabilities without negative effects on cell proliferation under in vitro conditions. Overall, these results suggest that PEU-coated scaffolds are an alternative to PCL for use in bone regeneration, providing a suitable environment to maximally induce osteogenesis.<\/jats:p>","DOI":"10.3390\/polym15102256","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T01:37:09Z","timestamp":1683769029000},"page":"2256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In Vitro Evaluation of Biphasic Calcium Phosphate Scaffolds Derived from Cuttlefish Bone Coated with Poly(ester urea) for Bone Tissue Regeneration"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5665-5271","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Pereira","sequence":"first","affiliation":[{"name":"IPN, Instituto Pedro Nunes, Associa\u00e7\u00e3o para a Inova\u00e7\u00e3o e Desenvolvimento em Ci\u00eancia e Tecnologia, Rua Pedro Nunes, 3030-199 Coimbra, Portugal"},{"name":"CEMMPRE, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima-P\u00f3lo II, 3030-790 Coimbra, Portugal"}]},{"given":"Ana S.","family":"Neto","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering\/CICECO\u2013Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Ana S.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"given":"In\u00eas","family":"Barros","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"CIBB-Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"III-Institute for Interdisciplinary Research, University of Coimbra, 3030-789 Coimbra, Portugal"}]},{"given":"Catarina","family":"Miranda","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"CIBB-Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"III-Institute for Interdisciplinary Research, University of Coimbra, 3030-789 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1172-4018","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Ramalho-Santos","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"DCV-Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5831-3307","authenticated-orcid":false,"given":"Lu\u00eds","family":"Pereira de Almeida","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"CIBB-Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Viravector-Viral Vector for Gene Transfer Core Facility, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-2809","authenticated-orcid":false,"given":"Jos\u00e9 M. F.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Materials and Ceramic Engineering\/CICECO\u2013Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Jorge F. J.","family":"Coelho","sequence":"additional","affiliation":[{"name":"IPN, Instituto Pedro Nunes, Associa\u00e7\u00e3o para a Inova\u00e7\u00e3o e Desenvolvimento em Ci\u00eancia e Tecnologia, Rua Pedro Nunes, 3030-199 Coimbra, Portugal"},{"name":"CEMMPRE, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima-P\u00f3lo II, 3030-790 Coimbra, Portugal"}]},{"given":"Ana C.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"CEMMPRE, Department of Chemical Engineering, University of Coimbra, Rua S\u00edlvio Lima-P\u00f3lo II, 3030-790 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S72","DOI":"10.1016\/j.injury.2020.07.040","article-title":"Synthetic and Bone tissue engineering graft substitutes: What is the future?","volume":"52","author":"Valtanen","year":"2021","journal-title":"Injury"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.bioactmat.2017.05.007","article-title":"Bone Grafts and Biomaterials Substitutes for Bone Defect Repair: A Review","volume":"2","author":"Wang","year":"2017","journal-title":"Bioact. 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