{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T06:31:51Z","timestamp":1762929111273,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT exploratory project B-EAMs: Exploring ElectroActive Microenvironments to re-sensitize Bacteria","award":["2022.02697.PTDC"],"award-info":[{"award-number":["2022.02697.PTDC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Indwelling medical devices, such as urinary catheters, often experience bacterial colonization, forming biofilms that resist antibiotics and the host\u2019s immune defenses through quorum sensing (QS), a chemical communication system. This study explores the development of antimicrobial coatings by immobilizing acylase, a quorum-quenching enzyme, on sandblasted polydimethylsiloxane (PDMS) surfaces. PDMS, commonly used in medical devices, was sandblasted to increase its surface roughness, enhancing acylase attachment. FTIR analysis confirmed that acylase retained its three-dimensional structure upon immobilization, preserving its enzymatic activity. The antibacterial efficacy of the coatings was tested against Pseudomonas aeruginosa (P. aeruginosa) (a common biofilm-forming pathogen), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). The results showed that sandblasted PDMS surfaces had improved bacterial adhesion due to increased focal adhesion points, but acylase-functionalized surfaces had significantly reduced bacterial attachment and biofilm formation. Notably, the coatings inhibited P. aeruginosa growth by 40% under static conditions, demonstrating the potential of acylase-functionalized PDMS for medical applications. This approach offers a promising strategy for creating antimicrobial surfaces that prevent biofilm-related infections in urinary catheters and other medical devices. The findings highlight the dual role of surface roughness in enhancing enzyme attachment while reducing bacterial adhesion through effective QS inhibition.<\/jats:p>","DOI":"10.3390\/polym17020182","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T06:13:07Z","timestamp":1736835187000},"page":"182","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acylase-Based Coatings on Sandblasted Polydimethylsiloxane-Based Materials for Antimicrobial Applications"],"prefix":"10.3390","volume":"17","author":[{"given":"Cl\u00e1udia A.","family":"Silva","sequence":"first","affiliation":[{"name":"Center for Micro-Electro Mechanical Systems (CMEMS), Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory in Biotechnology and Bioengineering and Microelectromechanical Systems, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6438-3889","authenticated-orcid":false,"given":"Joana","family":"Moreira","sequence":"additional","affiliation":[{"name":"Physics Centre of Minho and Porto Universities (CF-UM-UP), Campus Gualtar, University of Minho, 4710-057 Braga, Portugal"},{"name":"LaPMET\u2014Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal"},{"name":"Centre of Chemistry, Campus Gualtar, University of Minho, 4710-053 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5814-415X","authenticated-orcid":false,"given":"Marta","family":"Fernandes","sequence":"additional","affiliation":[{"name":"2C2T\u2014Centre for Textile Science and Technology, Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-4164","authenticated-orcid":false,"given":"Andrea","family":"Zille","sequence":"additional","affiliation":[{"name":"2C2T\u2014Centre for Textile Science and Technology, Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-5520","authenticated-orcid":false,"given":"Vanessa F.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Center for Micro-Electro Mechanical Systems (CMEMS), Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory in Biotechnology and Bioengineering and Microelectromechanical Systems, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5740-8627","authenticated-orcid":false,"given":"Md Julker","family":"Nine","sequence":"additional","affiliation":[{"name":"Centre of Chemistry, Campus Gualtar, University of Minho, 4710-053 Braga, Portugal"},{"name":"School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5000, Australia"}]},{"given":"Filipe S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Center for Micro-Electro Mechanical Systems (CMEMS), Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory in Biotechnology and Bioengineering and Microelectromechanical Systems, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1529-3702","authenticated-orcid":false,"given":"Margarida M.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Center for Micro-Electro Mechanical Systems (CMEMS), Campus Azur\u00e9m, University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS-Associate Laboratory in Biotechnology and Bioengineering and Microelectromechanical Systems, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.amjmed.2006.03.011","article-title":"Mechanisms of Antimicrobial Resistance in Bacteria","volume":"119","author":"Tenover","year":"2006","journal-title":"Am. 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