{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:34:42Z","timestamp":1767897282453,"version":"3.49.0"},"reference-count":23,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T00:00:00Z","timestamp":1667433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Forest Research Centre","award":["UIDB\/00239\/2020"],"award-info":[{"award-number":["UIDB\/00239\/2020"]}]},{"name":"Forest Research Centre","award":["PD\/BD\/128259\/2016"],"award-info":[{"award-number":["PD\/BD\/128259\/2016"]}]},{"DOI":"10.13039\/501100001871","name":"FCT SUSFOR Doctoral Programme","doi-asserted-by":"publisher","award":["UIDB\/00239\/2020"],"award-info":[{"award-number":["UIDB\/00239\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT SUSFOR Doctoral Programme","doi-asserted-by":"publisher","award":["PD\/BD\/128259\/2016"],"award-info":[{"award-number":["PD\/BD\/128259\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>The cuticular lipid compounds, usually named cuticular waxes, present in the cuticular layering of Quercus suber adult leaves were extracted with solvents of different polarities (n-hexane, dichloromethane and acetone) and analysed by GC\u2013MS. Q. suber leaves have a substantial cuticular wax layer (2.8% of leaf mass and 239 \u03bcg\/cm2), composed predominantly by terpenes (43\u201363% of all compounds), followed by aliphatic long chain molecules, mainly fatty acids, and by smaller amounts of aliphatic alcohols and n-alkanes. The major identified compound was lupeol (1.2% of leaves in n-hexane extract). The recovery and composition of cuticular lipids depended on the solvent and extraction time. The non-polar or weak polar solvents n-hexane and dichloromethane extracted similar lipid yields (77% and 86% of the total extract, respectively) while acetone solubilised other cellular compounds, namely sugars, with the lipid compounds representing 43% of the total extract. For cuticular lipids extraction, solvents with a low polarity such as n-hexane are the more suitable with an adequate extraction duration, e.g., n-hexane with a minimum extraction of 3 h.<\/jats:p>","DOI":"10.3390\/pr10112270","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T04:49:20Z","timestamp":1667450960000},"page":"2270","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Influence of Solvent and Extraction Time on Yield and Chemical Selectivity of Cuticular Waxes from Quercus suber Leaves"],"prefix":"10.3390","volume":"10","author":[{"given":"Rita","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"Centro de Estudos Florestais (CEF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"given":"Isabel","family":"Miranda","sequence":"additional","affiliation":[{"name":"Centro de Estudos Florestais (CEF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5393-4443","authenticated-orcid":false,"given":"Helena","family":"Pereira","sequence":"additional","affiliation":[{"name":"Centro de Estudos Florestais (CEF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.tplants.2008.03.003","article-title":"Building lipid barriers: Biosynthesis of cutin and suberin","volume":"13","author":"Pollard","year":"2008","journal-title":"Trends Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/jxb\/erq366","article-title":"Composition differences between epicuticular and intracuticular wax substructures: How do plants seal their epidermal surfaces?","volume":"62","author":"Buschhaus","year":"2011","journal-title":"J. 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