{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:29:47Z","timestamp":1760232587078,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>Previously, we showed that the crude methanol extracts of Leucas indica flowers exhibited antioxidant properties and in the current study, crude methanol flower extracts of L. indica showed anticancerous properties as evidenced cytotoxicity (MTT assay test) against the selected cancerous cell lines HeLa, HCT116, HL-60, and MCF-7. Therefore, further analysis was performed to isolate and purify the bioactive compound using activity-guided repeated fractionation of the methanol extract by silica gel column chromatography. After collection of different fractions, all the fractions were subjected to TLC analysis and the fractions which yielded the same compounds on TLC were further analyzed for physicochemical and spectroscopic analyses, e.g., UV, IR, 1H NMR, 13C NMR, COSY, HSQC, and mass spectroscopy. The bioactive compound isolated was elucidated as 6-hydroxy-3-(4-hydroxyphenyl)-7-(3,4,5-trihydroxy-6-)(hydroxymethyl)tetrahydro-2H-pyran-2yl)-4H-chromen-4-one. Based on the antioxidant and anticancerous properties, L. indica might be a promising source of useful natural products and the newly bioactive compound might offer opportunities to develop new anticancerous drugs.<\/jats:p>","DOI":"10.3390\/pr10112341","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T02:03:58Z","timestamp":1668045838000},"page":"2341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Purification, Structural Elucidation, and Anticancerous Properties of a Novel Flavonoid from Flowers of Leucas indica"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1848-3296","authenticated-orcid":false,"given":"Muthyam","family":"Sowjanya","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Andhra University, Visakhapatnam 530003, India"},{"name":"Department of Marine Biology, Vikram Simhapuri University, Nellore 524001, India"}]},{"given":"Bhagavathula","family":"Venkata Sandeep","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Andhra University, Visakhapatnam 530003, India"}]},{"given":"Kaduru","family":"Venkaiah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Vikrama Simhapuri University, Nellore 524001, India"}]},{"given":"Vijaya R.","family":"Dirisala","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Vignan\u2019s Foundation for Science, Technology and Research, Guntur 522213, India"}]},{"given":"Malapati Hanuma","family":"Reddy","sequence":"additional","affiliation":[{"name":"Department of Marine Biology, Vikram Simhapuri University, Nellore 524001, India"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4678-6443","authenticated-orcid":false,"given":"Jangampalli Adi","family":"Pradeepkiran","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Texas Tech University Health Science Centre, Lubbock, TX 79413, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5495-776X","authenticated-orcid":false,"given":"Sri Bhashyam","family":"Sainath","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Vikrama Simhapuri University, Nellore 524001, India"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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