{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:51:36Z","timestamp":1768827096572,"version":"3.49.0"},"reference-count":26,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o da Ci\u00eancia e da Tecnologia (FCT)","award":["PTDC\/EEI-EEE\/2846\/2021"],"award-info":[{"award-number":["PTDC\/EEI-EEE\/2846\/2021"]}]},{"name":"Funda\u00e7\u00e3o da Ci\u00eancia e da Tecnologia (FCT)","award":["EXPL\/EME-EME\/0732\/2021"],"award-info":[{"award-number":["EXPL\/EME-EME\/0732\/2021"]}]},{"name":"Funda\u00e7\u00e3o da Ci\u00eancia e da Tecnologia (FCT)","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"Funda\u00e7\u00e3o da 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(PIDDAC)","award":["PTDC\/EEI-EEE\/2846\/2021"],"award-info":[{"award-number":["PTDC\/EEI-EEE\/2846\/2021"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["EXPL\/EME-EME\/0732\/2021"],"award-info":[{"award-number":["EXPL\/EME-EME\/0732\/2021"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["UIDB\/00532\/2020"],"award-info":[{"award-number":["UIDB\/00532\/2020"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["UIDP\/00532\/2020"],"award-info":[{"award-number":["UIDP\/00532\/2020"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["UIDB\/04077\/2020"],"award-info":[{"award-number":["UIDB\/04077\/2020"]}]},{"name":"FCT\/MCTES (PIDDAC)","award":["UIDB\/04436\/2020"],"award-info":[{"award-number":["UIDB\/04436\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>The separation of red blood cells (RBCs) from blood plasma and the analysis of individual RBCs are of great importance, as they provide valuable information regarding the health of their donor. Recent developments in microfluidics and microfabrication have contributed to the fabrication of microsystems with complex features to promote the separation and analysis of RBCs. In this work, the separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid made by Brij L4 micelles and human RBCs separated from whole blood, suspended in a solution with hematocrits (Ht) of 0.5 and 1%. All the samples collected at the outlets of the device were experimentally analyzed and compared. The absorbance spectrum was also measured for the prepared blood samples. The results indicate that the tested blood analogue fluid has exhibited a flow behavior similar to that of blood. In addition, the optical absorbance spectrophotometry revealed that it was possible to evaluate the separation efficiency of the microfluidic device, concluding that the concentration of cells was lower at the most lateral outside outlets of the microchannel due to the cumulative effect of the multiple cross-flow filters.<\/jats:p>","DOI":"10.3390\/pr10122698","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T03:21:52Z","timestamp":1670988112000},"page":"2698","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7986-8934","authenticated-orcid":false,"given":"In\u00eas M.","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"IN+, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"}]},{"given":"In\u00eas","family":"Castro","sequence":"additional","affiliation":[{"name":"Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2971-910X","authenticated-orcid":false,"given":"Filipe","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9041-2188","authenticated-orcid":false,"given":"Vera","family":"Faustino","sequence":"additional","affiliation":[{"name":"Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8962-0710","authenticated-orcid":false,"given":"Susana O.","family":"Catarino","sequence":"additional","affiliation":[{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9801-7617","authenticated-orcid":false,"given":"Ana","family":"Moita","sequence":"additional","affiliation":[{"name":"IN+, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o Desenvolvimento e Inova\u00e7\u00e3o da Academia Militar, Academia Militar, Instituto Universit\u00e1rio Militar, 1169-203 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1830-0525","authenticated-orcid":false,"given":"Jo\u00e3o M.","family":"Miranda","sequence":"additional","affiliation":[{"name":"CEFT\u2014Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2460-0556","authenticated-orcid":false,"given":"Gra\u00e7a","family":"Minas","sequence":"additional","affiliation":[{"name":"Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2945-9267","authenticated-orcid":false,"given":"Patr\u00edcia C.","family":"Sousa","sequence":"additional","affiliation":[{"name":"INL\u2014International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3428-637X","authenticated-orcid":false,"given":"Rui","family":"Lima","sequence":"additional","affiliation":[{"name":"Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimar\u00e3es, Portugal"},{"name":"CEFT\u2014Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1039\/C5LC01159K","article-title":"Fundamentals and applications of inertial microfluidics: A review","volume":"16","author":"Zhang","year":"2016","journal-title":"Lab 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