{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T11:34:19Z","timestamp":1770377659479,"version":"3.49.0"},"reference-count":40,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/52532\/2014"],"award-info":[{"award-number":["SFRH\/BD\/52532\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["iNOVA4Health \u2013 UID\/Multi\/04462\/2013"],"award-info":[{"award-number":["iNOVA4Health \u2013 UID\/Multi\/04462\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011264","name":"FP7 People: Marie-Curie Actions","doi-asserted-by":"publisher","award":["FP7-PEOPLE-2013-IAPP- 612208"],"award-info":[{"award-number":["FP7-PEOPLE-2013-IAPP- 612208"]}],"id":[{"id":"10.13039\/100011264","id-type":"DOI","asserted-by":"publisher"}]},{"name":"RNEM \u2013 REDE\/1518\/REM\/2005, ROTEIRO\/0028\/ 2013","award":["LISBOA-01-0145-FEDER-022125"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-022125"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>Arbutus unedo L., commonly known as the strawberry-tree fruit, is an endemic species of the Mediterranean flora. Microwave extraction technology has been considered as a fast and \u201cgreen\u201d method for the production of extracts rich in bioactive compounds, although the energy consumption is high. To overcome this bottleneck, microwave was used as a pretreatment procedure in short time periods. This technique promotes the burst of intracellular vacuoles leading to an increase in the lixiviation of phenolic compounds. Different approaches were tested, namely a solvent-free irradiation (SFI), a solvent-assisted irradiation (SAI) and a pressurized solvent-assisted irradiation (PSAI). After irradiation, a solid\u2013liquid extraction procedure was performed using a mixture of water and ethanol. A kinetic evaluation of the total phenolic content (TPC) was performed using the Folin\u2013Ciocalteu method. For the total anthocyanin content, a UV-spectrophotometric method was used. HPLC-UV and LC-MS were used for TPC and identification of present compounds. Microwave irradiation led to an increase in TPC of extracts after SAI (52%) and PSAI (66%) along with a reduction in time of extraction from 30 min to less than 2 min. The anthocyanin content also increased by 66% for the SAI and PSAI extractions.<\/jats:p>","DOI":"10.3390\/pr8030298","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T09:26:41Z","timestamp":1583486801000},"page":"298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Phenolic Compounds Extraction of Arbutus unedo L.: Process Intensification by Microwave Pretreatment"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3405-802X","authenticated-orcid":false,"given":"Agostinho M. R. C.","family":"Alexandre","sequence":"first","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Avenida da Republica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4888-9414","authenticated-orcid":false,"given":"Ana A.","family":"Matias","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"}]},{"given":"Maria Ros\u00e1rio","family":"Bronze","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2781-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Avenida da Republica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Farm\u00e1cia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3047-2389","authenticated-orcid":false,"given":"Maria Jose","family":"Cocero","sequence":"additional","affiliation":[{"name":"Institute of Bioeconomy BioEcoUva, High Pressure Processes Group, Chemical Engineering and Environmental Technology, University of Valladolid, 47011 Valladolid, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2439-4772","authenticated-orcid":false,"given":"Rafael","family":"Mato","sequence":"additional","affiliation":[{"name":"Institute of Bioeconomy BioEcoUva, High Pressure Processes Group, Chemical Engineering and Environmental Technology, University of Valladolid, 47011 Valladolid, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.3390\/nu2020214","article-title":"Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo)","volume":"2","author":"Fortalezas","year":"2010","journal-title":"Nutrients"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10234","DOI":"10.1021\/jf062230o","article-title":"Phenolics of Arbutus unedo L. 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