{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T07:09:09Z","timestamp":1777964949577,"version":"3.51.4"},"reference-count":228,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["POCI-01-0247-FEDER-033784"],"award-info":[{"award-number":["POCI-01-0247-FEDER-033784"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04730\/2020"],"award-info":[{"award-number":["UIDB\/04730\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IF\/01093\/2014\/CP1249\/CT0003"],"award-info":[{"award-number":["IF\/01093\/2014\/CP1249\/CT0003"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"COMPETE2020 \u2013 Programa Operacional Competitividade e Internacionaliza\u00e7\u00e3o (POCI)","award":["POCI-01-0247-FEDER-033784"],"award-info":[{"award-number":["POCI-01-0247-FEDER-033784"]}]},{"name":"Marie Sk\u0142odowska-Curie grant agreement","award":["867473"],"award-info":[{"award-number":["867473"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>Several microalgae species have been exploited due to their great biotechnological potential for the production of a range of biomolecules that can be applied in a large variety of industrial sectors. However, the major challenge of biotechnological processes is to make them economically viable, through the production of commercially valuable compounds. Most of these compounds are accumulated inside the cells, requiring efficient technologies for their extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g., supercritical fluid extraction, ultrasound-assisted extraction, pulsed electric fields, among others) and processes without solvents are seeking to establish sustainable and scalable technologies to obtain target products from microalgae with high efficiency and purity. This article reviews the currently available approaches reported in literature, highlighting some examples covering recent granted patents for the microalgae\u2019s components extraction, recovery and purification, at small and large scales, in accordance with the worldwide trend of transition to bio-based products.<\/jats:p>","DOI":"10.3390\/pr9010010","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T20:39:29Z","timestamp":1608669569000},"page":"10","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":155,"title":["Microalgae Biomolecules: Extraction, Separation and Purification Methods"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1490-6594","authenticated-orcid":false,"given":"Priscila S.","family":"Corr\u00eaa","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto (FEUP), R. Dr. Roberto Frias s\/n, 4200-465 Porto, Portugal"},{"name":"CIETI, School of Engineering (ISEP), Polytechnic Institute of Porto (P.Porto), R. Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2530-6327","authenticated-orcid":false,"given":"Wilson G.","family":"Morais J\u00fanior","sequence":"additional","affiliation":[{"name":"CIETI, School of Engineering (ISEP), Polytechnic Institute of Porto (P.Porto), R. Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"given":"Ant\u00f3nio A.","family":"Martins","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto (FEUP), R. Dr. Roberto Frias s\/n, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2185-6401","authenticated-orcid":false,"given":"N\u00eddia S.","family":"Caetano","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto (FEUP), R. Dr. Roberto Frias s\/n, 4200-465 Porto, Portugal"},{"name":"CIETI, School of Engineering (ISEP), Polytechnic Institute of Porto (P.Porto), R. Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8696-8925","authenticated-orcid":false,"given":"Teresa M.","family":"Mata","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto (FEUP), R. Dr. Roberto Frias s\/n, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"735562","DOI":"10.1016\/j.aquaculture.2020.735562","article-title":"Microalgae for biotechnological applications: Cultivation, harvesting and biomass processing","volume":"528","author":"Gorgich","year":"2020","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.rser.2009.07.020","article-title":"Microalgae for biodiesel production and other applications: A review","volume":"14","author":"Mata","year":"2010","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Luque, R., and Melero, J.A. (2012). Microalgae processing for biodiesel production. Advances in Biodiesel Production: Processes and Technologies, Elsevier Ltd.. 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